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- W2085147312 abstract "Continuous and uniform exposure to an atmosphere of dry SO3 effectively chars chromatograms with convenience, safety and speed. The sandblasted, concave surface of a heat resistant glass lid is coated with fuming sulfuric acid. The lid encloses the thin layer chromatography plate while it is heated by a hot plate. Cholesterol, oleic acid and triolein show SO3 char and char-fluorescence detection limits much lower and carbon yields more nearly equal than those reported to be obtained with conventional spray char reagents. Liquid reagent does not touch the layer. It remains clean, dry and permanent. Freedom from excess acid lends stability to the intensity of char-fluorescence spots. Because of lower temperature requirements, SO3 char probably can be extended to include substances which are too volatile for acid spray char. A guide to the selection of conditions for the detection, identification and quantitation of char, charfluorescence and char-color spots is given. Additional areas of investigation with SO3 as well as other reagent fumes are indicated." @default.
- W2085147312 created "2016-06-24" @default.
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- W2085147312 date "1971-11-01" @default.
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- W2085147312 title "Charring with sulfur trioxide for the improved visualization and quantitation of thin layer chromatograms" @default.
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- W2085147312 doi "https://doi.org/10.1007/bf02638536" @default.
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