FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085149823> ?p ?o ?g. }

• W2085149823 endingPage "290" @default.
• W2085149823 startingPage "277" @default.
• W2085149823 abstract "The binding of [14]glycine to rat brain-cortex membrane fragments, incubated in artificial cerebrospinal fluid, was studiedin vitro by means of a nitrocellulose filter assay. The membranes were obtained from the large granule fraction (P2) of a brain-cortex homogenate, which was osmotically shocked and the larger membrane fractions isolated by centrifugation. Initial binding velocity lasts for about 2 min and equilibrium is reached in 10 min. The binding reaction is reversible, and [14C]glycine can be displaced by an excess of [12C]glycine or by dilution. Binding is strongly dependent on temperature and on sodium ions. The latter activate the binding process in a cooperative manner. Two binding components may be discerned: one with high affinity for glycine (Km = 40 ± 8 μM) and one with lower affinity. Lowering the sodium concentration to 60 mM increases theKm of the high-affinity component to 59 μM, with no change in Vmax. The bound product is, after incubating the membranes at 37 °C for 10 min, 85% glycine. A large fraction of it may be released by hypoosmotic media. The affinity of the receptor for different amino acids is in the order: glycine, alanine, serine, leucine. Negligible affinity is shown towards ß-alanine, lysine or arginine. γ-Aminobutyric acid (GABA), glutamate and aspartate inhibit glycine binding by a mechanism which is probably non-competitive. This affinity pattern is the same as that of the receptor involved in the transport of small neutral amino acids in brain slices. The sodium and temperature dependence are also characteristic of transport phenomena. The main conclusion of this work is that in brain-cortex only transport receptors, as opposed to synaptic receptors, can be demonstrated for glycine." @default.
• W2085149823 created "2016-06-24" @default.
• W2085149823 creator A5003487515 @default.
• W2085149823 creator A5039880692 @default.
• W2085149823 date "1975-10-01" @default.
• W2085149823 modified "2023-10-17" @default.
• W2085149823 title "Interaction of glycine with brain-cortex membrane fragments: Kinetics, ionic requirements and amino acid specificity" @default.
• W2085149823 cites W1488488806 @default.
• W2085149823 cites W1498746516 @default.
• W2085149823 cites W1775749144 @default.
• W2085149823 cites W1963509455 @default.
• W2085149823 cites W1970480967 @default.
• W2085149823 cites W1971637773 @default.
• W2085149823 cites W1980144951 @default.
• W2085149823 cites W1981604210 @default.
• W2085149823 cites W1985640796 @default.
• W2085149823 cites W2015539609 @default.
• W2085149823 cites W2018155268 @default.
• W2085149823 cites W2021958520 @default.
• W2085149823 cites W2028535521 @default.
• W2085149823 cites W2030731082 @default.
• W2085149823 cites W2040453625 @default.
• W2085149823 cites W2040742860 @default.
• W2085149823 cites W2042822339 @default.
• W2085149823 cites W2059435612 @default.
• W2085149823 cites W2060226757 @default.
• W2085149823 cites W2063471775 @default.
• W2085149823 cites W2072344341 @default.
• W2085149823 cites W2073749288 @default.
• W2085149823 cites W2073837185 @default.
• W2085149823 cites W2079331809 @default.
• W2085149823 cites W2086874051 @default.
• W2085149823 cites W2089425407 @default.
• W2085149823 cites W2097039450 @default.
• W2085149823 cites W2124990645 @default.
• W2085149823 cites W2136724071 @default.
• W2085149823 cites W2277435727 @default.
• W2085149823 cites W2338947998 @default.
• W2085149823 cites W2439604354 @default.
• W2085149823 cites W2473420716 @default.
• W2085149823 doi "https://doi.org/10.1016/0006-8993(75)90450-3" @default.
• W2085149823 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1175047" @default.
• W2085149823 hasPublicationYear "1975" @default.
• W2085149823 type Work @default.
• W2085149823 sameAs 2085149823 @default.
• W2085149823 citedByCount "17" @default.
• W2085149823 crossrefType "journal-article" @default.
• W2085149823 hasAuthorship W2085149823A5003487515 @default.
• W2085149823 hasAuthorship W2085149823A5039880692 @default.
• W2085149823 hasConcept C107824862 @default.
• W2085149823 hasConcept C12554922 @default.
• W2085149823 hasConcept C152900378 @default.
• W2085149823 hasConcept C170493617 @default.
• W2085149823 hasConcept C178790620 @default.
• W2085149823 hasConcept C181199279 @default.
• W2085149823 hasConcept C185592680 @default.
• W2085149823 hasConcept C2776414213 @default.
• W2085149823 hasConcept C2777756961 @default.
• W2085149823 hasConcept C2779856020 @default.
• W2085149823 hasConcept C41625074 @default.
• W2085149823 hasConcept C515207424 @default.
• W2085149823 hasConcept C537181965 @default.
• W2085149823 hasConcept C55493867 @default.
• W2085149823 hasConcept C86803240 @default.
• W2085149823 hasConceptScore W2085149823C107824862 @default.
• W2085149823 hasConceptScore W2085149823C12554922 @default.
• W2085149823 hasConceptScore W2085149823C152900378 @default.
• W2085149823 hasConceptScore W2085149823C170493617 @default.
• W2085149823 hasConceptScore W2085149823C178790620 @default.
• W2085149823 hasConceptScore W2085149823C181199279 @default.
• W2085149823 hasConceptScore W2085149823C185592680 @default.
• W2085149823 hasConceptScore W2085149823C2776414213 @default.
• W2085149823 hasConceptScore W2085149823C2777756961 @default.
• W2085149823 hasConceptScore W2085149823C2779856020 @default.
• W2085149823 hasConceptScore W2085149823C41625074 @default.
• W2085149823 hasConceptScore W2085149823C515207424 @default.
• W2085149823 hasConceptScore W2085149823C537181965 @default.
• W2085149823 hasConceptScore W2085149823C55493867 @default.
• W2085149823 hasConceptScore W2085149823C86803240 @default.
• W2085149823 hasIssue "2" @default.
• W2085149823 hasLocation W20851498231 @default.
• W2085149823 hasLocation W20851498232 @default.
• W2085149823 hasOpenAccess W2085149823 @default.
• W2085149823 hasPrimaryLocation W20851498231 @default.
• W2085149823 hasRelatedWork W111279495 @default.
• W2085149823 hasRelatedWork W1487965268 @default.
• W2085149823 hasRelatedWork W1984054744 @default.
• W2085149823 hasRelatedWork W2007281810 @default.
• W2085149823 hasRelatedWork W2026586334 @default.
• W2085149823 hasRelatedWork W2040249061 @default.
• W2085149823 hasRelatedWork W2067456796 @default.
• W2085149823 hasRelatedWork W2077064019 @default.
• W2085149823 hasRelatedWork W2150367211 @default.
• W2085149823 hasRelatedWork W1959733034 @default.
• W2085149823 hasVolume "97" @default.
• W2085149823 isParatext "false" @default.
• W2085149823 isRetracted "false" @default.
• W2085149823 magId "2085149823" @default.

• next