FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085149898> ?p ?o ?g. }

• W2085149898 abstract "Abstract G-protein coupled receptor 43 ( GPR43 ) recognizes short chain fatty acids and is implicated in obesity, colitis, asthma and arthritis. Here, we present the first full characterization of the GPR43 promoter and 5′-UTR. 5′-RACE of the GPR43 transcript identified the transcription start site (TSS) and a 124 bp 5′-UTR followed by a 1335 bp intron upstream of the ATG start codon. The sequence spanning -4560 to +68 bp relative to the GPR43 TSS was found to contain strong promoter activity, increasing luciferase reporter expression by >100-fold in U937 monocytes. Stepwise deletions further narrowed the putative GPR43 promoter (−451 to +68). Site-directed mutagenesis identified XBP1 as a core cis element, the mutation of which abrogated transcriptional activity. Mutations of predicted CREB, CHOP, NFAT and STAT5 binding sites, partially reduced promoter activity. ChIP assays confirmed the binding of XBP1 to the endogenous GPR43 promoter. Consistently, GPR43 expression is reduced in monocytes upon siRNA-knockdown of XBP1, while A549 cells overexpressing XBP1 displayed elevated GPR43 levels. Based on its ability to activate XBP1, we predicted and confirmed that TNFα induces GPR43 expression in human monocytes. Altogether, our findings form the basis for strategic modulation of GPR43 expression, with a view to regulate GPR43 -associated diseases." @default.
• W2085149898 created "2016-06-24" @default.
• W2085149898 creator A5007620364 @default.
• W2085149898 creator A5016756488 @default.
• W2085149898 creator A5091509054 @default.
• W2085149898 date "2015-01-30" @default.
• W2085149898 modified "2023-10-15" @default.
• W2085149898 title "The short-chain fatty acid receptor GPR43 is transcriptionally regulated by XBP1 in human monocytes" @default.
• W2085149898 cites W1483073028 @default.
• W2085149898 cites W1511265793 @default.
• W2085149898 cites W1965985248 @default.
• W2085149898 cites W1969218281 @default.
• W2085149898 cites W1972691065 @default.
• W2085149898 cites W1980130314 @default.
• W2085149898 cites W1985024845 @default.
• W2085149898 cites W1991074258 @default.
• W2085149898 cites W1991332015 @default.
• W2085149898 cites W1993037755 @default.
• W2085149898 cites W1993688639 @default.
• W2085149898 cites W2000117132 @default.
• W2085149898 cites W2001938165 @default.
• W2085149898 cites W2007078899 @default.
• W2085149898 cites W2019419945 @default.
• W2085149898 cites W2035421462 @default.
• W2085149898 cites W2040481343 @default.
• W2085149898 cites W2040852375 @default.
• W2085149898 cites W2044227662 @default.
• W2085149898 cites W2057039181 @default.
• W2085149898 cites W2060185417 @default.
• W2085149898 cites W2061725286 @default.
• W2085149898 cites W2068511150 @default.
• W2085149898 cites W2080503511 @default.
• W2085149898 cites W2093756809 @default.
• W2085149898 cites W2099662390 @default.
• W2085149898 cites W2110524342 @default.
• W2085149898 cites W2112835086 @default.
• W2085149898 cites W2113254662 @default.
• W2085149898 cites W2114570899 @default.
• W2085149898 cites W2121236896 @default.
• W2085149898 cites W2122734844 @default.
• W2085149898 cites W2125647281 @default.
• W2085149898 cites W2127799088 @default.
• W2085149898 cites W2133230812 @default.
• W2085149898 cites W2135232490 @default.
• W2085149898 cites W2135301154 @default.
• W2085149898 cites W2141215852 @default.
• W2085149898 cites W2142638534 @default.
• W2085149898 cites W2144509989 @default.
• W2085149898 cites W2144701449 @default.
• W2085149898 cites W2149185205 @default.
• W2085149898 cites W2151845029 @default.
• W2085149898 cites W2153550523 @default.
• W2085149898 cites W2162704444 @default.
• W2085149898 cites W2165067288 @default.
• W2085149898 cites W2165261749 @default.
• W2085149898 cites W2168842536 @default.
• W2085149898 cites W2170348331 @default.
• W2085149898 cites W2321091703 @default.
• W2085149898 doi "https://doi.org/10.1038/srep08134" @default.
• W2085149898 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4311239" @default.
• W2085149898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25633224" @default.
• W2085149898 hasPublicationYear "2015" @default.
• W2085149898 type Work @default.
• W2085149898 sameAs 2085149898 @default.
• W2085149898 citedByCount "35" @default.
• W2085149898 countsByYear W20851498982016 @default.
• W2085149898 countsByYear W20851498982017 @default.
• W2085149898 countsByYear W20851498982018 @default.
• W2085149898 countsByYear W20851498982019 @default.
• W2085149898 countsByYear W20851498982020 @default.
• W2085149898 countsByYear W20851498982021 @default.
• W2085149898 countsByYear W20851498982022 @default.
• W2085149898 countsByYear W20851498982023 @default.
• W2085149898 crossrefType "journal-article" @default.
• W2085149898 hasAuthorship W2085149898A5007620364 @default.
• W2085149898 hasAuthorship W2085149898A5016756488 @default.
• W2085149898 hasAuthorship W2085149898A5091509054 @default.
• W2085149898 hasBestOaLocation W20851498981 @default.
• W2085149898 hasConcept C101762097 @default.
• W2085149898 hasConcept C104317684 @default.
• W2085149898 hasConcept C111335760 @default.
• W2085149898 hasConcept C138885662 @default.
• W2085149898 hasConcept C150194340 @default.
• W2085149898 hasConcept C153911025 @default.
• W2085149898 hasConcept C179926584 @default.
• W2085149898 hasConcept C2781331208 @default.
• W2085149898 hasConcept C41895202 @default.
• W2085149898 hasConcept C54009773 @default.
• W2085149898 hasConcept C54355233 @default.
• W2085149898 hasConcept C54458228 @default.
• W2085149898 hasConcept C67705224 @default.
• W2085149898 hasConcept C86803240 @default.
• W2085149898 hasConceptScore W2085149898C101762097 @default.
• W2085149898 hasConceptScore W2085149898C104317684 @default.
• W2085149898 hasConceptScore W2085149898C111335760 @default.
• W2085149898 hasConceptScore W2085149898C138885662 @default.
• W2085149898 hasConceptScore W2085149898C150194340 @default.
• W2085149898 hasConceptScore W2085149898C153911025 @default.
• W2085149898 hasConceptScore W2085149898C179926584 @default.
• W2085149898 hasConceptScore W2085149898C2781331208 @default.

• next