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• W2085152042 endingPage "277" @default.
• W2085152042 startingPage "269" @default.
• W2085152042 abstract "Nanodiscs are a promising system for studying gas-phase and solution complexes of membrane proteins and lipids. We previously demonstrated that native electrospray ionization allows mass spectral analysis of intact Nanodisc complexes at single lipid resolution. This report details an improved theoretical framework for interpreting and deconvoluting native mass spectra of Nanodisc lipoprotein complexes. In addition to the intrinsic lipid count and charge distributions, Nanodisc mass spectra are significantly shaped by constructive overlap of adjacent charge states at integer multiples of the lipid mass. We describe the mathematical basis for this effect and develop a probability-based algorithm to deconvolute the underlying mass and charge distributions. The probability-based deconvolution algorithm is applied to a series of dimyristoylphosphatidylcholine Nanodisc native mass spectra and used to provide a quantitative picture of the lipid loss in gas-phase fragmentation." @default.
• W2085152042 created "2016-06-24" @default.
• W2085152042 creator A5031479644 @default.
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• W2085152042 creator A5081446055 @default.
• W2085152042 creator A5089783459 @default.
• W2085152042 date "2013-12-19" @default.
• W2085152042 modified "2023-10-18" @default.
• W2085152042 title "Interpretation and Deconvolution of Nanodisc Native Mass Spectra" @default.
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• W2085152042 doi "https://doi.org/10.1007/s13361-013-0782-y" @default.
• W2085152042 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3918181" @default.
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• W2085152042 hasPublicationYear "2013" @default.
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