FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085157974> ?p ?o ?g. }

• W2085157974 endingPage "892" @default.
• W2085157974 startingPage "876" @default.
• W2085157974 abstract "Cell-cell communication in astroglial syncytia is mediated by intracellular Ca(2+) ([Ca(2+)](i)) responses elicited by extracellular signaling molecules as well as by diverse physical and chemical stimuli. Despite the evidence that astrocytic swelling promotes [Ca(2+)](i) elevation through Ca(2+) influx, the molecular identity of the channel protein underlying this response is still elusive. Here we report that primary cultured cortical astrocytes express the transient receptor potential vanilloid-related channel 4 (TRPV 4), a Ca(2+)-permeable cation channel gated by a variety of stimuli, including cell swelling. Immunoblot and confocal microscopy analyses confirmed the presence of the channel protein and its localization in the plasma membrane. TRPV4 was functional because the selective TRPV4 agonist 4-alpha-phorbol 12,13-didecanoate (4alphaPDD) activated an outwardly rectifying cation current with biophysical and pharmacological properties that overlapped those of recombinant human TRPV4 expressed in COS cells. Moreover, 4alphaPDD and hypotonic challenge promoted [Ca(2+)](i) elevation mediated by influx of extracellular Ca(2+). This effect was abolished by low micromolar concentration of the TRPV4 inhibitor Ruthenium Red. Immunofluorescence and immunogold electron microscopy of rat brain revealed that TRPV4 was enriched in astrocytic processes of the superficial layers of the neocortex and in astrocyte end feet facing pia and blood vessels. Collectively, these data indicate that cultured cortical astroglia express functional TRPV4 channels. They also demonstrate that TRPV4 is particularly abundant in astrocytic membranes at the interface between brain and extracerebral liquid spaces. Consistent with its roles in other tissues, these results support the view that TRPV4 might participate in astroglial osmosensation and thus play a key role in brain volume homeostasis." @default.
• W2085157974 created "2016-06-24" @default.
• W2085157974 creator A5006618435 @default.
• W2085157974 creator A5032846457 @default.
• W2085157974 creator A5033595095 @default.
• W2085157974 creator A5074111902 @default.
• W2085157974 creator A5074921206 @default.
• W2085157974 creator A5087096006 @default.
• W2085157974 creator A5087625230 @default.
• W2085157974 date "2007-09-01" @default.
• W2085157974 modified "2023-10-16" @default.
• W2085157974 title "Expression and functional characterization of transient receptor potential vanilloid-related channel 4 (TRPV4) in rat cortical astrocytes" @default.
• W2085157974 cites W1511198581 @default.
• W2085157974 cites W1535419310 @default.
• W2085157974 cites W1545384325 @default.
• W2085157974 cites W1576543894 @default.
• W2085157974 cites W1654040390 @default.
• W2085157974 cites W1707717597 @default.
• W2085157974 cites W1840415699 @default.
• W2085157974 cites W1963614683 @default.
• W2085157974 cites W1964119066 @default.
• W2085157974 cites W1965529878 @default.
• W2085157974 cites W1966329284 @default.
• W2085157974 cites W1969326379 @default.
• W2085157974 cites W1969403218 @default.
• W2085157974 cites W1969509501 @default.
• W2085157974 cites W1970629236 @default.
• W2085157974 cites W1972737184 @default.
• W2085157974 cites W1974155494 @default.
• W2085157974 cites W1978942142 @default.
• W2085157974 cites W1988759455 @default.
• W2085157974 cites W1991509237 @default.
• W2085157974 cites W1992601827 @default.
• W2085157974 cites W1992726458 @default.
• W2085157974 cites W1993818203 @default.
• W2085157974 cites W1997480962 @default.
• W2085157974 cites W1998106094 @default.
• W2085157974 cites W2000616741 @default.
• W2085157974 cites W2003380041 @default.
• W2085157974 cites W2004630293 @default.
• W2085157974 cites W2007654857 @default.
• W2085157974 cites W2009667219 @default.
• W2085157974 cites W2011557706 @default.
• W2085157974 cites W2016633207 @default.
• W2085157974 cites W2021815265 @default.
• W2085157974 cites W2022262895 @default.
• W2085157974 cites W2022867171 @default.
• W2085157974 cites W2023966975 @default.
• W2085157974 cites W2026607657 @default.
• W2085157974 cites W2029766879 @default.
• W2085157974 cites W2031677113 @default.
• W2085157974 cites W2031943544 @default.
• W2085157974 cites W2033794046 @default.
• W2085157974 cites W2037993046 @default.
• W2085157974 cites W2049247347 @default.
• W2085157974 cites W2052482154 @default.
• W2085157974 cites W2055048326 @default.
• W2085157974 cites W2055162787 @default.
• W2085157974 cites W2061017773 @default.
• W2085157974 cites W2061210518 @default.
• W2085157974 cites W2061267962 @default.
• W2085157974 cites W2063063986 @default.
• W2085157974 cites W2064576113 @default.
• W2085157974 cites W2083897778 @default.
• W2085157974 cites W2083977043 @default.
• W2085157974 cites W2084415764 @default.
• W2085157974 cites W2086188945 @default.
• W2085157974 cites W2088314252 @default.
• W2085157974 cites W2091014895 @default.
• W2085157974 cites W2091057443 @default.
• W2085157974 cites W2097824035 @default.
• W2085157974 cites W2104587256 @default.
• W2085157974 cites W2107750976 @default.
• W2085157974 cites W2110265830 @default.
• W2085157974 cites W2113405252 @default.
• W2085157974 cites W2118522143 @default.
• W2085157974 cites W2118622904 @default.
• W2085157974 cites W2120679080 @default.
• W2085157974 cites W2127302126 @default.
• W2085157974 cites W2128158693 @default.
• W2085157974 cites W2133108447 @default.
• W2085157974 cites W2137154133 @default.
• W2085157974 cites W2137290540 @default.
• W2085157974 cites W2141260882 @default.
• W2085157974 cites W2141892357 @default.
• W2085157974 cites W2149429155 @default.
• W2085157974 cites W2150070295 @default.
• W2085157974 cites W2154873371 @default.
• W2085157974 cites W2156165752 @default.
• W2085157974 cites W2156197458 @default.
• W2085157974 cites W2157139186 @default.
• W2085157974 cites W2160036343 @default.
• W2085157974 cites W2169915413 @default.
• W2085157974 cites W2273259361 @default.
• W2085157974 cites W4240955431 @default.
• W2085157974 doi "https://doi.org/10.1016/j.neuroscience.2007.06.039" @default.
• W2085157974 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17719182" @default.
• W2085157974 hasPublicationYear "2007" @default.

• next