FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085159563> ?p ?o ?g. }

• W2085159563 endingPage "147" @default.
• W2085159563 startingPage "141" @default.
• W2085159563 abstract "Angiotensin-converting enzyme 2 (ACE2 or ACEH) is a novel angiotensin-converting enzyme-related carboxypeptidase that cleaves a single amino acid from angiotensin I, des-Arg bradykinin, and many other bioactive peptides. Using des-Arg bradykinin as a template, we designed a series of intramolecularly quenched fluorogenic peptide substrates for ACE2. The general structure of the substrates was F-X-Q, in which F was the fluorescent group, Abz, Q was the quenching group (either Phe(NO2) or Tyr(NO2)), and X was the intervening peptide. These substrates were selectively cleaved by recombinant human ACE2, as shown by MS and HPLC. Quenching efficiency increased as the peptide sequence was shortened from 8 to 3 aa, and also when Tyr(NO2) was used as a quenching group instead of Phe(NO2). Two of the optimized substrates, TBC5180 and TBC5182, produced a signal:noise ratio of better than 20 when hydrolyzed by ACE2. Kinetic measurements with ACE2 were as follows: TBC5180, Km=58μM and kcat/Km=1.3×105M−1s−1; TBC5182, Km=23μM and kcat/Km=3.5×104M−1s−1. Thus, based on hydrolysis rate, TBC5180 was a better substrate than TBC5182. However, TBC5180 was also hydrolyzed by ACE, whereas TBC5182 was not cleaved, suggesting that TBC5182 was a selective for ACE2. We conclude that these two peptides can be used as fluorescent substrates for high-throughput screening for selective inhibitors of ACE2 enzyme." @default.
• W2085159563 created "2016-06-24" @default.
• W2085159563 creator A5015165992 @default.
• W2085159563 creator A5016575685 @default.
• W2085159563 creator A5022492311 @default.
• W2085159563 creator A5039398140 @default.
• W2085159563 creator A5056693715 @default.
• W2085159563 creator A5071142294 @default.
• W2085159563 date "2003-01-01" @default.
• W2085159563 modified "2023-10-09" @default.
• W2085159563 title "Development of intramolecularly quenched fluorescent peptides as substrates of angiotensin-converting enzyme 2" @default.
• W2085159563 cites W1990917512 @default.
• W2085159563 cites W1991356362 @default.
• W2085159563 cites W2000040025 @default.
• W2085159563 cites W2000846353 @default.
• W2085159563 cites W2022029597 @default.
• W2085159563 cites W2025672718 @default.
• W2085159563 cites W2026460796 @default.
• W2085159563 cites W2034035538 @default.
• W2085159563 cites W2036081511 @default.
• W2085159563 cites W2040570452 @default.
• W2085159563 cites W2048647186 @default.
• W2085159563 cites W2070197928 @default.
• W2085159563 cites W2091839905 @default.
• W2085159563 cites W2092093169 @default.
• W2085159563 cites W2101610495 @default.
• W2085159563 cites W2141134151 @default.
• W2085159563 cites W2149146712 @default.
• W2085159563 cites W2161399617 @default.
• W2085159563 doi "https://doi.org/10.1016/s0003-2697(02)00461-x" @default.
• W2085159563 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12531198" @default.
• W2085159563 hasPublicationYear "2003" @default.
• W2085159563 type Work @default.
• W2085159563 sameAs 2085159563 @default.
• W2085159563 citedByCount "17" @default.
• W2085159563 countsByYear W20851595632013 @default.
• W2085159563 countsByYear W20851595632015 @default.
• W2085159563 countsByYear W20851595632017 @default.
• W2085159563 countsByYear W20851595632018 @default.
• W2085159563 crossrefType "journal-article" @default.
• W2085159563 hasAuthorship W2085159563A5015165992 @default.
• W2085159563 hasAuthorship W2085159563A5016575685 @default.
• W2085159563 hasAuthorship W2085159563A5022492311 @default.
• W2085159563 hasAuthorship W2085159563A5039398140 @default.
• W2085159563 hasAuthorship W2085159563A5056693715 @default.
• W2085159563 hasAuthorship W2085159563A5071142294 @default.
• W2085159563 hasConcept C121332964 @default.
• W2085159563 hasConcept C121745418 @default.
• W2085159563 hasConcept C134018914 @default.
• W2085159563 hasConcept C170493617 @default.
• W2085159563 hasConcept C181199279 @default.
• W2085159563 hasConcept C185592680 @default.
• W2085159563 hasConcept C18903297 @default.
• W2085159563 hasConcept C27016395 @default.
• W2085159563 hasConcept C2777289219 @default.
• W2085159563 hasConcept C2779281246 @default.
• W2085159563 hasConcept C2779591629 @default.
• W2085159563 hasConcept C2908929049 @default.
• W2085159563 hasConcept C41183919 @default.
• W2085159563 hasConcept C55493867 @default.
• W2085159563 hasConcept C56856141 @default.
• W2085159563 hasConcept C62520636 @default.
• W2085159563 hasConcept C71240020 @default.
• W2085159563 hasConcept C84393581 @default.
• W2085159563 hasConcept C86803240 @default.
• W2085159563 hasConcept C91881484 @default.
• W2085159563 hasConcept C94412978 @default.
• W2085159563 hasConceptScore W2085159563C121332964 @default.
• W2085159563 hasConceptScore W2085159563C121745418 @default.
• W2085159563 hasConceptScore W2085159563C134018914 @default.
• W2085159563 hasConceptScore W2085159563C170493617 @default.
• W2085159563 hasConceptScore W2085159563C181199279 @default.
• W2085159563 hasConceptScore W2085159563C185592680 @default.
• W2085159563 hasConceptScore W2085159563C18903297 @default.
• W2085159563 hasConceptScore W2085159563C27016395 @default.
• W2085159563 hasConceptScore W2085159563C2777289219 @default.
• W2085159563 hasConceptScore W2085159563C2779281246 @default.
• W2085159563 hasConceptScore W2085159563C2779591629 @default.
• W2085159563 hasConceptScore W2085159563C2908929049 @default.
• W2085159563 hasConceptScore W2085159563C41183919 @default.
• W2085159563 hasConceptScore W2085159563C55493867 @default.
• W2085159563 hasConceptScore W2085159563C56856141 @default.
• W2085159563 hasConceptScore W2085159563C62520636 @default.
• W2085159563 hasConceptScore W2085159563C71240020 @default.
• W2085159563 hasConceptScore W2085159563C84393581 @default.
• W2085159563 hasConceptScore W2085159563C86803240 @default.
• W2085159563 hasConceptScore W2085159563C91881484 @default.
• W2085159563 hasConceptScore W2085159563C94412978 @default.
• W2085159563 hasIssue "2" @default.
• W2085159563 hasLocation W20851595631 @default.
• W2085159563 hasLocation W20851595632 @default.
• W2085159563 hasOpenAccess W2085159563 @default.
• W2085159563 hasPrimaryLocation W20851595631 @default.
• W2085159563 hasRelatedWork W1976408724 @default.
• W2085159563 hasRelatedWork W1990404564 @default.
• W2085159563 hasRelatedWork W1998108893 @default.
• W2085159563 hasRelatedWork W2030365219 @default.
• W2085159563 hasRelatedWork W2046776259 @default.

• next