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• W2085184401 abstract "Abstract In order to decrease significantly the oxygen affinity of human hemoglobin, we have associated the mutation βF41Y with another point mutation also known to decrease the oxygen affinity of Hb. We have synthesized a recombinant Hb (rHb) with two mutations in the β chains: rHb βF41Y,K66T. In the absence of 2,3-diphosphoglycerate, additive effects of the mutations are evident, since the doubly mutated Hb exhibits a larger decrease in oxygen affinity than for the individual single mutations. In the presence of 2,3-diphosphoglycerate, the second mutation did not significantly increase the P 50 value relative to the single mutations. However, the kinetics of CO binding still indicate combined effects on the allosteric equilibrium, as evidenced by more of the slow bimolecular phase characteristic of binding to the deoxy conformation. Dimer-tetramer equilibrium studies indicate an increase in stability of the mutants relative to rHb A; the double mutant rHb βF41Y,K66T at pH 7.5 showed a K 4,2 value of 0.26 μm. Despite the lower oxygen affinity, the single mutant βF41Y and double mutant βF41Y,K66T show only a moderate increase of 20% in the autoxidation rate. These mutations are thus of interest in developing a Hb-based blood substitute." @default.
• W2085184401 created "2016-06-24" @default.
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• W2085184401 date "1999-09-01" @default.
• W2085184401 modified "2023-09-29" @default.
• W2085184401 title "Additive Effects of β Chain Mutations in Low Oxygen Affinity Hemoglobin βF41Y,K66T" @default.
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• W2085184401 doi "https://doi.org/10.1074/jbc.274.36.25550" @default.
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