Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085186887> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2085186887 endingPage "3526" @default.
- W2085186887 startingPage "3518" @default.
- W2085186887 abstract "Phospholipid scramblase 1 (PLSCR1) is a multiply palmitoylated, Ca(2+)-binding, endofacial plasma membrane protein originally identified by its capacity to accelerate transbilayer movement of membrane phospholipids. We recently reported that when palmitoylation of PLSCR1 does not occur, it is localized to the nucleus rather than the plasma membrane. Nuclear localization of PLSCR1 was also observed upon induction of its de novo synthesis by cytokines such as interferon alpha that activate the PLSCR1 gene. Despite its capacity to enter the nucleus, its sequence does not predict a nuclear localization signal. To gain insight into the mechanism and potential significance of nuclear PLSCR1, we investigated the conditions required for its import and retention in the nucleus. We show that nuclear localization of PLSCR1 is dependent on cytosolic factors and energy. Furthermore, we show that PLSCR1 is specifically transported into the nucleus by the importin alpha/beta import pathway, and binds directly and with high affinity to importin alpha. Analysis of deletion mutants suggested that the NLS of PLSCR1 is between residues 242 and 290 and, furthermore, that a peptide within this region encompassing residues (257)GKISKHWTGI(266) is sufficient for nuclear import when conjugated to BSA. In addition, in intact cells, mutation of positively charged amino acids within this putative NLS in the full-length protein completely blocked its entry into the nucleus, consistent with its role in targeting PLSCR1 to the nucleus. Release of PLSCR1 from the nucleus was only observed after treatment of cells with both detergent and an elevated NaCl concentration, or following DNase treatment of the nucleus, suggesting ionic interactions of PLSCR1 with a nuclear component bound to genomic DNA or directly with genomic DNA. Purified PLSCR1 was also found to bind directly to a genomic DNA-cellulose conjugate, and its elution from DNA also required an elevated NaCl concentration. These data support a mechanism of receptor-mediated nuclear import of PLSCR1 and suggest a potential nuclear function for this plasma membrane protein." @default.
- W2085186887 created "2016-06-24" @default.
- W2085186887 creator A5004352838 @default.
- W2085186887 creator A5016683563 @default.
- W2085186887 creator A5018166397 @default.
- W2085186887 creator A5026350256 @default.
- W2085186887 creator A5063062861 @default.
- W2085186887 date "2004-03-01" @default.
- W2085186887 modified "2023-09-23" @default.
- W2085186887 title "Phospholipid Scramblase 1 Is Imported into the Nucleus by a Receptor-Mediated Pathway and Interacts with DNA<sup>,</sup>" @default.
- W2085186887 cites W1902070349 @default.
- W2085186887 cites W1971934332 @default.
- W2085186887 cites W2020572083 @default.
- W2085186887 cites W2063174199 @default.
- W2085186887 cites W2064884184 @default.
- W2085186887 cites W2081055733 @default.
- W2085186887 cites W2083964225 @default.
- W2085186887 cites W2103653609 @default.
- W2085186887 cites W2105987719 @default.
- W2085186887 doi "https://doi.org/10.1021/bi0356911" @default.
- W2085186887 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15035622" @default.
- W2085186887 hasPublicationYear "2004" @default.
- W2085186887 type Work @default.
- W2085186887 sameAs 2085186887 @default.
- W2085186887 citedByCount "61" @default.
- W2085186887 countsByYear W20851868872012 @default.
- W2085186887 countsByYear W20851868872013 @default.
- W2085186887 countsByYear W20851868872014 @default.
- W2085186887 countsByYear W20851868872015 @default.
- W2085186887 countsByYear W20851868872016 @default.
- W2085186887 countsByYear W20851868872017 @default.
- W2085186887 countsByYear W20851868872018 @default.
- W2085186887 countsByYear W20851868872019 @default.
- W2085186887 countsByYear W20851868872020 @default.
- W2085186887 countsByYear W20851868872021 @default.
- W2085186887 countsByYear W20851868872022 @default.
- W2085186887 crossrefType "journal-article" @default.
- W2085186887 hasAuthorship W2085186887A5004352838 @default.
- W2085186887 hasAuthorship W2085186887A5016683563 @default.
- W2085186887 hasAuthorship W2085186887A5018166397 @default.
- W2085186887 hasAuthorship W2085186887A5026350256 @default.
- W2085186887 hasAuthorship W2085186887A5063062861 @default.
- W2085186887 hasConcept C133882971 @default.
- W2085186887 hasConcept C153911025 @default.
- W2085186887 hasConcept C181199279 @default.
- W2085186887 hasConcept C185592680 @default.
- W2085186887 hasConcept C188810444 @default.
- W2085186887 hasConcept C2778918659 @default.
- W2085186887 hasConcept C2779637612 @default.
- W2085186887 hasConcept C2780114586 @default.
- W2085186887 hasConcept C2780723820 @default.
- W2085186887 hasConcept C28859421 @default.
- W2085186887 hasConcept C41625074 @default.
- W2085186887 hasConcept C49805395 @default.
- W2085186887 hasConcept C55493867 @default.
- W2085186887 hasConcept C59157529 @default.
- W2085186887 hasConcept C65871279 @default.
- W2085186887 hasConcept C86803240 @default.
- W2085186887 hasConcept C95444343 @default.
- W2085186887 hasConcept C98539663 @default.
- W2085186887 hasConceptScore W2085186887C133882971 @default.
- W2085186887 hasConceptScore W2085186887C153911025 @default.
- W2085186887 hasConceptScore W2085186887C181199279 @default.
- W2085186887 hasConceptScore W2085186887C185592680 @default.
- W2085186887 hasConceptScore W2085186887C188810444 @default.
- W2085186887 hasConceptScore W2085186887C2778918659 @default.
- W2085186887 hasConceptScore W2085186887C2779637612 @default.
- W2085186887 hasConceptScore W2085186887C2780114586 @default.
- W2085186887 hasConceptScore W2085186887C2780723820 @default.
- W2085186887 hasConceptScore W2085186887C28859421 @default.
- W2085186887 hasConceptScore W2085186887C41625074 @default.
- W2085186887 hasConceptScore W2085186887C49805395 @default.
- W2085186887 hasConceptScore W2085186887C55493867 @default.
- W2085186887 hasConceptScore W2085186887C59157529 @default.
- W2085186887 hasConceptScore W2085186887C65871279 @default.
- W2085186887 hasConceptScore W2085186887C86803240 @default.
- W2085186887 hasConceptScore W2085186887C95444343 @default.
- W2085186887 hasConceptScore W2085186887C98539663 @default.
- W2085186887 hasIssue "12" @default.
- W2085186887 hasLocation W20851868871 @default.
- W2085186887 hasLocation W20851868872 @default.
- W2085186887 hasOpenAccess W2085186887 @default.
- W2085186887 hasPrimaryLocation W20851868871 @default.
- W2085186887 hasRelatedWork W1951758308 @default.
- W2085186887 hasRelatedWork W1967521176 @default.
- W2085186887 hasRelatedWork W1976758932 @default.
- W2085186887 hasRelatedWork W2041424868 @default.
- W2085186887 hasRelatedWork W2085186887 @default.
- W2085186887 hasRelatedWork W2343383277 @default.
- W2085186887 hasRelatedWork W2612456237 @default.
- W2085186887 hasRelatedWork W3165851349 @default.
- W2085186887 hasRelatedWork W4210427470 @default.
- W2085186887 hasRelatedWork W4362553554 @default.
- W2085186887 hasVolume "43" @default.
- W2085186887 isParatext "false" @default.
- W2085186887 isRetracted "false" @default.
- W2085186887 magId "2085186887" @default.
- W2085186887 workType "article" @default.