FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085187310> ?p ?o ?g. }

• W2085187310 endingPage "12143" @default.
• W2085187310 startingPage "12133" @default.
• W2085187310 abstract "RecA protein of Escherichia coli plays an essential role in homologous recombination of DNA strands. To analyze the interaction of RecA with single-stranded DNA (ssDNA), we performed a fluorescence competition assay employing 1-anilinonaphthalene-8-sulfonic acid (ANS) as an extrinsic fluorescent probe. ANS bound to RecA at three sites, leading to enhancement of ANS fluorescence. Addition of synthetic polynucleotides to the RecA−ANS complex in the absence of a nucleotide quenched the ANS fluorescence, indicating displacement of ANS molecules by ssDNA. Less effective quenching by poly(dA) suggests that the nucleoprotein filament on poly(dA) may differ from those on poly(dT) and poly(dC). A titration experiment with poly(dT) and poly(dA) showed clear stoichiometric binding of 3.5 nucleotides per protein. The site size for poly(dC) was 7.0, which could be explained by the formation of a double helix of poly(dC). ATP and other nucleotides also displaced the ANS. To identify ANS-binding sites, ANS was incorporated into RecA by UV irradiation, and fluorescent peptides were isolated from the proteolytic digest. Sequence analysis suggested that ANS binds to or near the ATP-binding region. These results suggest that the fluorescence quenching and photoincorporation assay using ANS may be useful for the analysis of the interaction of a protein and its ligand." @default.
• W2085187310 created "2016-06-24" @default.
• W2085187310 creator A5025116997 @default.
• W2085187310 creator A5072018588 @default.
• W2085187310 date "1998-08-08" @default.
• W2085187310 modified "2023-09-25" @default.
• W2085187310 title "Probing of DNA-Binding Sites of <i>Escherichia coli</i> RecA Protein Utilizing 1-Anilinonaphthalene-8-sulfonic Acid" @default.
• W2085187310 cites W1492402815 @default.
• W2085187310 cites W1492465042 @default.
• W2085187310 cites W1506534753 @default.
• W2085187310 cites W1513848044 @default.
• W2085187310 cites W1521772215 @default.
• W2085187310 cites W1521958691 @default.
• W2085187310 cites W1523956889 @default.
• W2085187310 cites W1546428176 @default.
• W2085187310 cites W1554158004 @default.
• W2085187310 cites W1562088891 @default.
• W2085187310 cites W1598630167 @default.
• W2085187310 cites W1622496100 @default.
• W2085187310 cites W1915342015 @default.
• W2085187310 cites W1976444339 @default.
• W2085187310 cites W2021787419 @default.
• W2085187310 cites W2025602823 @default.
• W2085187310 cites W2034571171 @default.
• W2085187310 cites W2040242310 @default.
• W2085187310 cites W2066925054 @default.
• W2085187310 cites W2077980347 @default.
• W2085187310 cites W2126857023 @default.
• W2085187310 cites W2134102804 @default.
• W2085187310 cites W2145304961 @default.
• W2085187310 cites W2150484524 @default.
• W2085187310 cites W2167973356 @default.
• W2085187310 cites W2316159845 @default.
• W2085187310 cites W280694366 @default.
• W2085187310 doi "https://doi.org/10.1021/bi980541p" @default.
• W2085187310 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9724525" @default.
• W2085187310 hasPublicationYear "1998" @default.
• W2085187310 type Work @default.
• W2085187310 sameAs 2085187310 @default.
• W2085187310 citedByCount "10" @default.
• W2085187310 countsByYear W20851873102013 @default.
• W2085187310 countsByYear W20851873102016 @default.
• W2085187310 crossrefType "journal-article" @default.
• W2085187310 hasAuthorship W2085187310A5025116997 @default.
• W2085187310 hasAuthorship W2085187310A5072018588 @default.
• W2085187310 hasConcept C102744134 @default.
• W2085187310 hasConcept C104317684 @default.
• W2085187310 hasConcept C107824862 @default.
• W2085187310 hasConcept C111921641 @default.
• W2085187310 hasConcept C121332964 @default.
• W2085187310 hasConcept C121745418 @default.
• W2085187310 hasConcept C12554922 @default.
• W2085187310 hasConcept C179104552 @default.
• W2085187310 hasConcept C184866935 @default.
• W2085187310 hasConcept C185592680 @default.
• W2085187310 hasConcept C512185932 @default.
• W2085187310 hasConcept C547475151 @default.
• W2085187310 hasConcept C552990157 @default.
• W2085187310 hasConcept C55493867 @default.
• W2085187310 hasConcept C62520636 @default.
• W2085187310 hasConcept C86803240 @default.
• W2085187310 hasConcept C91881484 @default.
• W2085187310 hasConceptScore W2085187310C102744134 @default.
• W2085187310 hasConceptScore W2085187310C104317684 @default.
• W2085187310 hasConceptScore W2085187310C107824862 @default.
• W2085187310 hasConceptScore W2085187310C111921641 @default.
• W2085187310 hasConceptScore W2085187310C121332964 @default.
• W2085187310 hasConceptScore W2085187310C121745418 @default.
• W2085187310 hasConceptScore W2085187310C12554922 @default.
• W2085187310 hasConceptScore W2085187310C179104552 @default.
• W2085187310 hasConceptScore W2085187310C184866935 @default.
• W2085187310 hasConceptScore W2085187310C185592680 @default.
• W2085187310 hasConceptScore W2085187310C512185932 @default.
• W2085187310 hasConceptScore W2085187310C547475151 @default.
• W2085187310 hasConceptScore W2085187310C552990157 @default.
• W2085187310 hasConceptScore W2085187310C55493867 @default.
• W2085187310 hasConceptScore W2085187310C62520636 @default.
• W2085187310 hasConceptScore W2085187310C86803240 @default.
• W2085187310 hasConceptScore W2085187310C91881484 @default.
• W2085187310 hasIssue "35" @default.
• W2085187310 hasLocation W20851873101 @default.
• W2085187310 hasLocation W20851873102 @default.
• W2085187310 hasOpenAccess W2085187310 @default.
• W2085187310 hasPrimaryLocation W20851873101 @default.
• W2085187310 hasRelatedWork W1552269101 @default.
• W2085187310 hasRelatedWork W1984478674 @default.
• W2085187310 hasRelatedWork W1999818538 @default.
• W2085187310 hasRelatedWork W2015508394 @default.
• W2085187310 hasRelatedWork W2034979205 @default.
• W2085187310 hasRelatedWork W2040388799 @default.
• W2085187310 hasRelatedWork W2083202085 @default.
• W2085187310 hasRelatedWork W2128442121 @default.
• W2085187310 hasRelatedWork W2134855082 @default.
• W2085187310 hasRelatedWork W2405713808 @default.
• W2085187310 hasVolume "37" @default.
• W2085187310 isParatext "false" @default.
• W2085187310 isRetracted "false" @default.
• W2085187310 magId "2085187310" @default.

• next