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- W2085188771 abstract "Fungal chitosan was extracted from Aspergillus niger mycelia. The produced chitosan was characterized with deacetylation degree of 89.2%, a molecular weight of 2.4 × 10 4 Da, and 96.0% solubility in 1% acetic acid solution. The antibacterial activity of fungal chitosan was evaluated against two foodborne pathogens, that is, Salmonella typhimurium and Staphylococcus aureus , using the established antibacterial assays, for example, zone of growth inhibition and agar plat count tests, and using 2,3,5,-triphenyltetrazolium chloride (TTC) as chromogenic marker for qualitative and quantitative determining of antibacterial potentiality. The TTC (0.5% w/v) was added, at concentration of 10%, to cultured broth, containing chitosan with different concentrations then the formed formazan was separated. The formation of red formazan could be considered as a qualitative indication for antibacterial activity, whereas the measurement of color intensity for the resuspended red formazan, using spectrophotometer at 480 nm, provided a quantitative evidence for the strength of the used antibacterial agent. Regarding the rapidity, technical simplicity, and cost-effectiveness, TTC assay could be recommended as an efficient alternative method for qualitative and quantitative determination of chitosan antibacterial activity and could be suggested for general evaluation of antibacterial agents." @default.
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- W2085188771 date "2013-06-03" @default.
- W2085188771 modified "2023-09-24" @default.
- W2085188771 title "Tetrazolium/Formazan Test as an Efficient Method to Determine Fungal Chitosan Antimicrobial Activity" @default.
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- W2085188771 doi "https://doi.org/10.1155/2013/753692" @default.
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