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• W2085191605 abstract "Replacement of amino acids 4187–4628 in the skeletal muscle Ca2+ release channel (skeletal ryanodine receptor (RyR1)), including nearly all of divergent region 1 (amino acids 4254–4631), with the corresponding cardiac ryanodine receptor (RyR2) sequence leads to increased sensitivity of channel activation by caffeine and Ca2+ and to decreased sensitivity of channel inactivation by elevated Ca2+ (Du, G. G., and MacLennan, D. H. (1999) J. Biol. Chem. 274, 26120–26126). In further investigations, this region was subdivided by the construction of new chimeras, and alterations in channel function were detected by measurement of the caffeine dependence of in vivo Ca2+ release and the Ca2+ dependence of [3H]ryanodine binding. Chimera RF10a (amino acids 4187–4381) had a lower EC50value for activation by caffeine, and RF10c (4557–4628) had a higher EC50 value, whereas the EC50 value for chimera RF10b (4382–4556) was unchanged. Chimeras RF10b and RF10c were more sensitive to activation by Ca2+, whereas RF10a was less sensitive to inactivation by Ca2+, implicating RF10b and RF10c in Ca2+ activation and RF10a in Ca2+inactivation. Deletion of much of divergent region 1 sequence to create mutant Δ4274–4535 led to higher caffeine and Ca2+sensitivity of channel activation and to lower Ca2+sensitivity for inactivation. Thus, deletion results demonstrate that caffeine, Ca2+, and ryanodine binding sites are not located in amino acids 4274–4535. Nevertheless, the properties of the deletion and chimeric mutants demonstrate that amino acids 4274–4535 and three shorter sequences in this region (F10a, amino acids 4187–4381; F10b, 4382–4556; and F10c, 4557–4628) in RyR1 modulate Ca2+ and caffeine sensitivity of the Ca2+ release channel. Replacement of amino acids 4187–4628 in the skeletal muscle Ca2+ release channel (skeletal ryanodine receptor (RyR1)), including nearly all of divergent region 1 (amino acids 4254–4631), with the corresponding cardiac ryanodine receptor (RyR2) sequence leads to increased sensitivity of channel activation by caffeine and Ca2+ and to decreased sensitivity of channel inactivation by elevated Ca2+ (Du, G. G., and MacLennan, D. H. (1999) J. Biol. Chem. 274, 26120–26126). In further investigations, this region was subdivided by the construction of new chimeras, and alterations in channel function were detected by measurement of the caffeine dependence of in vivo Ca2+ release and the Ca2+ dependence of [3H]ryanodine binding. Chimera RF10a (amino acids 4187–4381) had a lower EC50value for activation by caffeine, and RF10c (4557–4628) had a higher EC50 value, whereas the EC50 value for chimera RF10b (4382–4556) was unchanged. Chimeras RF10b and RF10c were more sensitive to activation by Ca2+, whereas RF10a was less sensitive to inactivation by Ca2+, implicating RF10b and RF10c in Ca2+ activation and RF10a in Ca2+inactivation. Deletion of much of divergent region 1 sequence to create mutant Δ4274–4535 led to higher caffeine and Ca2+sensitivity of channel activation and to lower Ca2+sensitivity for inactivation. Thus, deletion results demonstrate that caffeine, Ca2+, and ryanodine binding sites are not located in amino acids 4274–4535. Nevertheless, the properties of the deletion and chimeric mutants demonstrate that amino acids 4274–4535 and three shorter sequences in this region (F10a, amino acids 4187–4381; F10b, 4382–4556; and F10c, 4557–4628) in RyR1 modulate Ca2+ and caffeine sensitivity of the Ca2+ release channel. ryanodine receptor skeletal muscle RyR isoform cardiac muscle RyR isoform 3-[(3-cholamidopropyl)dimethylammonio-]-1-propanesulfonic acid human embryonic kidney cell line 293 cassette 10 divergent region 1 picosiemens The mechanism of excitation-contraction coupling differs in cardiac and skeletal muscles: in cardiac muscle, contraction requires extracellular Ca2+ influx through the dihydropyridine receptor to activate the Ca2+ release channel (ryanodine receptor (RyR))1 in the sarcoplasmic reticulum membrane and cause Ca2+-induced Ca2+ release from the sarcoplasmic reticulum; in skeletal muscle, contraction does not require extracellular Ca2+ and appears to be induced through the dihydropyridine receptor via physical interaction with the ryanodine receptor (1.Fleischer S. Inui M. Annu. Rev. Biophys. Biophys. Chem. 1989; 18: 333-364Crossref PubMed Scopus (444) Google Scholar, 2.Franzini-Armstrong C. Protasi F. Physiol. Rev. 1997; 77: 699-729Crossref PubMed Scopus (596) Google Scholar). Despite these physiological differences, Ca2+ is a basic modulator of both RyR1 and RyR2. Both channels are also modulated by other endogenous and exogenous modulators, such as ATP, calmodulin, Mg2+, ruthenium red, and ryanodine, but the extent of modulation by Ca2+, ATP, Mg2+, and ruthenium red differs between RyR1 and RyR2 (3.Coronado R. Morrissette J. Sukhareva M. Vaughan D.M. Am. J. Physiol. 1994; 266: C1485-C1504Crossref PubMed Google Scholar, 4.Meissner G. Annu. Rev. Physiol. 1994; 56: 485-508Crossref PubMed Scopus (843) Google Scholar, 5.Zucchi R. Ronca-Testoni S. Pharmacol. Rev. 1997; 49: 53-98PubMed Google Scholar). The molecular mechanisms underlying the interactions of these modulatory ligands with RyRs are not yet known. The amino acid sequences of RyR1 and RyR2, deduced from cDNA cloning (6.Takeshima H. Nishimura S. Matsumoto T. Ishida H. Kangawa K. Minamino N. Matsuo H. Ueda M. Hanaoka M. Hirose T. Numa S. Nature. 1989; 339: 439-445Crossref PubMed Scopus (868) Google Scholar, 7.Zorzato F. Fujii J. Otsu K. Phillips M. Green N.M. Lai F.A. Meissner G. MacLennan D.H. J. Biol. Chem. 1990; 265: 2244-2256Abstract Full Text PDF PubMed Google Scholar, 8.Otsu K. Willard H.F. Khanna V.K. Zorzato F. Green N.M. MacLennan D.H. J. Biol. Chem. 1990; 265: 13472-13483Abstract Full Text PDF PubMed Google Scholar, 9.Nakai J. Imagawa T. Hakamat Y. Shigekawa M. Takeshima H. Numa S. FEBS Lett. 1990; 271: 169-177Crossref PubMed Scopus (290) Google Scholar), are 66% identical, with regions of high sequence identity and regions of high diversity. If different physiological functions and pharmacological properties could be correlated with different sequences, binding sites for modulating agents in RyR might be identified, leading to a better understanding of structure/function relationships within the molecule. Structure/function analysis of ryanodine receptors has identified several important regions in the molecule. Malignant hyperthermia and central core disease mutations, found in the sequences lying between amino acids 35 and 614 and 2163 and 2458 (10.Loke J. MacLennan D.H. Am. J. Med. 1998; 104: 470-486Abstract Full Text Full Text PDF PubMed Scopus (177) Google Scholar), alter sensitivity of the channel to caffeine and halothane (11.Tong J. Oyamada H. Demaurex N. Grinstein S. McCarthy T.V. MacLennan D.H. J. Biol. Chem. 1997; 272: 26332-26339Abstract Full Text Full Text PDF PubMed Scopus (219) Google Scholar, 12.Tong J. McCarthy T.V. MacLennan D.H. J. Biol. Chem. 1999; 274: 693-702Abstract Full Text Full Text PDF PubMed Scopus (164) Google Scholar). Another central core disease mutation has been found in the C terminus in predicted transmembrane 9 (TM9) or its adjacent lumenal domain (13.Lynch P.J. Tong J. Lehane M. Mallet A. Giblin L. Heffron J.J. Vaughan P. Zafra G. MacLennan D.H. McCarthy T.V. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 4164-4169Crossref PubMed Scopus (206) Google Scholar). Evidence that the Ca2+ sensor lies in TM2 has been presented by Chenet al. (14.Chen S.R.W. Ebisawa K. Li X. Zhang L. J. Biol. Chem. 1998; 273: 14675-14678Abstract Full Text Full Text PDF PubMed Scopus (108) Google Scholar), who showed that mutation of Glu3987in RyR3 (equivalent to Glu4032 in RyR1) caused a huge decrease in Ca2+ sensitivity. Other mutations of acidic amino acids in TM2, TM7, and TM10 have also been shown to block caffeine and 4-chloro-m-cresol activation and high affinity ryanodine binding, but single channel function was not analyzed (15.Du G.G. MacLennan D.H. J. Biol. Chem. 1998; 273: 31867-31872Abstract Full Text Full Text PDF PubMed Scopus (53) Google Scholar). Evidence that TM9 is a Ca2+ channel pore has been presented by Chen and co-workers (16.Zhao M. Li P. Li X. Zhang L. Winkfein R.J. Chen S.R.W. J. Biol. Chem. 1999; 274: 25971-25974Abstract Full Text Full Text PDF PubMed Scopus (169) Google Scholar), showing that a single mutation, G4824A, reduced single channel conductance from 798 pS for the wild type channel to 22 pS. The mutant channel was modulated by Ca2+, Mg2+, ATP, caffeine, ruthenium red, and ryanodine. Co-expression of wild type and G4824A mutant proteins yielded single channels with intermediate unitary conductances. This is in line with observations in the central core disease mutation (13.Lynch P.J. Tong J. Lehane M. Mallet A. Giblin L. Heffron J.J. Vaughan P. Zafra G. MacLennan D.H. McCarthy T.V. Proc. Natl. Acad. Sci. U. S. A. 1999; 96: 4164-4169Crossref PubMed Scopus (206) Google Scholar). Deletion of the N-terminal sequences of RyR1 revealed that one-fifth of the C-terminal sequence contains structures sufficient to form a functional Ca2+ release channel, but the N-terminal sequence also regulates the release channel (17.Bhat M.B. Zhao J. Takeshima H. Ma J. Biophys. J. 1997; 73: 1329-1336Abstract Full Text PDF PubMed Scopus (116) Google Scholar). Deletion of 3 amino acids at the C terminus of RyR1 resulted in decreased activities, whereas deletion of 15 amino acids yielded an inactive RyR (18.Gao L. Tripathy A. Lu X. Meissner G. FEBS Lett. 1997; 412: 223-226Crossref PubMed Scopus (56) Google Scholar). In earlier studies, we used chimeric molecules to exploit the differences between Ca2+ inactivation profiles of RyR1 and RyR2, allowing us to localize the low affinity Ca2+ binding site(s) that inactivates the channel between amino acids 3726 and 5037 (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar). These conclusions have been supported in recent studies by Nakaiet al. (20.Nakai J. Gao L. Xu L. Xin C. Pasek D.A. Meissner G. FEBS Lett. 1999; 459: 154-158Crossref PubMed Scopus (27) Google Scholar). We also found that RyR chimeras containing the C-terminal sequence of RyR2 were more sensitive to Ca2+activation than RyR1. This was unexpected, because Ca2+activation in native or recombinant RyR1 and RyR2 is similar (3.Coronado R. Morrissette J. Sukhareva M. Vaughan D.M. Am. J. Physiol. 1994; 266: C1485-C1504Crossref PubMed Google Scholar, 5.Zucchi R. Ronca-Testoni S. Pharmacol. Rev. 1997; 49: 53-98PubMed Google Scholar,21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). RyR2 and RyR1/RyR2 chimeras containing the RyR2 C terminus were also more sensitive to caffeine activation than RyR1 (3.Coronado R. Morrissette J. Sukhareva M. Vaughan D.M. Am. J. Physiol. 1994; 266: C1485-C1504Crossref PubMed Google Scholar, 5.Zucchi R. Ronca-Testoni S. Pharmacol. Rev. 1997; 49: 53-98PubMed Google Scholar, 19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar), suggesting that the caffeine activation site might also be located in this region. Caffeine appears to activate RyR by increasing Ca2+ sensitivity (3.Coronado R. Morrissette J. Sukhareva M. Vaughan D.M. Am. J. Physiol. 1994; 266: C1485-C1504Crossref PubMed Google Scholar, 5.Zucchi R. Ronca-Testoni S. Pharmacol. Rev. 1997; 49: 53-98PubMed Google Scholar). Clearly, sequence changes in this region affect the sensitivity of the channel to activation by Ca2+ and caffeine and to inactivation by elevated Ca2+. Within the C-terminal sequence studied earlier (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar), amino acids 4187–4628 are most closely associated with activation and inactivation of RyR1. This sequence overlaps nearly all of divergent region 1 (D1) (amino acids 4254–4631), one of the three most divergent regions between RyR1 and RyR2. The other divergent regions in RyR1 are D2 (amino acids 1342–1403) and D3 (amino acids 1872–1923) (22.Sorrentino V. Volpe P. Trends Pharmacol. Sci. 1993; 14: 98-103Abstract Full Text PDF PubMed Scopus (276) Google Scholar). D2 and D3 sequences have been related to excitation-contraction coupling (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar,23.Yamazawa T. Takeshima H. Shimuta M. Iino M. J. Biol. Chem. 1997; 272: 8161-8164Abstract Full Text Full Text PDF PubMed Scopus (76) Google Scholar, 24.Nakai J. Sekiguchi N. Rando T.A. Allen P.D. Beam K.G. J. Biol. Chem. 1998; 273: 13403-13406Abstract Full Text Full Text PDF PubMed Scopus (156) Google Scholar). D3 is unlikely to be involved in Ca2+ inactivation (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar, 20.Nakai J. Gao L. Xu L. Xin C. Pasek D.A. Meissner G. FEBS Lett. 1999; 459: 154-158Crossref PubMed Scopus (27) Google Scholar). The role of D1 is unknown. In an attempt to clarify the role of D1 in Ca2+ release channel function, we have subdivided D1 into three small chimeras and constructed a deletion mutant in this region. We tested the Ca2+ dependence of high affinity [3H]ryanodine binding to these mutant proteins to look at both Ca2+ activation and Ca2+ inactivation and we measured in vivo Ca2+ release induced by caffeine with Ca2+ photometry. Our results show that the high affinity ryanodine binding site and caffeine and Ca2+activation sites are not located in the sequence between 4274–4535 but suggest that part of the Ca2+ inactivation site resides in the sequence that includes amino acids 4187–4381. Pfu DNA polymerase, restriction endonucleases, and other DNA modifying enzymes were from Stratagene, Roche Molecular Biochemicals, New England Biolabs, Promega, and Amersham Pharmacia Biotech; Fura-2 acetoxymethyl ester (AM) was from Molecular Probes; caffeine and protease inhibitors were from Sigma; [3H]ryanodine was from NEN Life Science Products; unlabeled ryanodine was from Calbiochem; CHAPS was from Bio-Rad; and phosphatidylcholine was from Avanti Polar Lipids. The expression vector pcDNA 3.1(–) was from Invitrogen. Monoclonal antibody 34C (mAb 34C) was a kind gift from Dr. Judith Airey (25.Airey J.A. Beck C.F. Murakami K. Tanksley S.J. Deerinck T.J. Ellisman M.H. Sutko J.L. J. Biol. Chem. 1990; 265: 14187-14194Abstract Full Text PDF PubMed Google Scholar). All other reagents were of reagent grade or the highest grade available. The methods for expression of cDNAs encoding rabbit skeletal muscle RyR1 and cardiac muscle RyR2 were described previously (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar, 26.Chen S.R. Vaughan D.M. Airey J.A. Coronado R. MacLennan D.H. Biochemistry. 1993; 32: 3743-3753Crossref PubMed Scopus (49) Google Scholar, 27.Chen S.R. Leong P. Imredy J.P. Bartlett C. Zhang L. MacLennan D.H. Biophys. J. 1997; 73: 1904-1912Abstract Full Text PDF PubMed Scopus (33) Google Scholar). The boundaries used in construction of chimeric RYR cDNAs from RYR1 andRYR2 and of deletion-mutated RYR cDNAs are outlined in Fig. 1. The three regions most divergent in amino acid sequence between RyR1 and RyR2 are labeled as D1–D3 (22.Sorrentino V. Volpe P. Trends Pharmacol. Sci. 1993; 14: 98-103Abstract Full Text PDF PubMed Scopus (276) Google Scholar) in Fig. 1. Part of cassette 10 (C10), lying between NheI andXcaI in a modified RYR1 cDNA (11.Tong J. Oyamada H. Demaurex N. Grinstein S. McCarthy T.V. MacLennan D.H. J. Biol. Chem. 1997; 272: 26332-26339Abstract Full Text Full Text PDF PubMed Scopus (219) Google Scholar) and encoding amino acids 4187–4628 encompassing the D1 region, is designated F10. The construction of chimeric RyR involving F10 has been described previously (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar). F10a contains RyR1 amino acids 4187–4381, F10b contains RyR1 amino acids 4382–4556, and F10c contains RyR1 amino acids 4557–4628. RYR2 fragments were amplified using aPfu polymerase-based polymerase chain reaction in which restriction endonuclease sites were introduced at each end (NheI-SphI for F10a,SphI-NruI for F10b, andNruI-XcaI for F10c). The NruI site in F10 was obtained by site-directed mutagenesis with polymerase chain reaction. These fragments were inserted into their corresponding locations in pBS-F10 to form pBS-F10a, pBS-F10b, and pBS-F10c. The F10 fragments in the last three constructs were further cleaved and inserted into pBS-RyR1 to form pBS-RF10a, pBS-RF10b, and pBS-RF10c. The full-length chimeric constructs were subcloned into pcDNA 3.1(−) with XbaI and HindIII to obtain pcDNA-RF10a, pcDNA-RF10b, and pcDNA-RF10c. The construction of a deletion mutant involving the D1 region was carried out in RYR1 cDNA cassette 10 (NheI-ClaI) (Fig. 1). In C10, there are fiveNarI sites (12819, 12840, 12873, 13107, and 13605). To obtain Δ4274–4535-C10, C10 was digested with NarI to delete DNA sequence 12819–13605 and self-ligated with T4 DNA ligase. The deleted C10 was inserted into the corresponding region of pBS-RyR1, and the resulting cDNA sequence was then excised and inserted into pcDNA3.1(−) with XbaI and HindIII to form Δ4274–4535-R1. These chimeric inserts and the deletion mutant were confirmed by DNA sequencing and restriction enzyme-digestion mapping. Culture of HEK-293 cells and cDNA transfection by the calcium phosphate precipitation method (28.Chen C. Okayama H. Mol. Cell. Biol. 1987; 7: 2745-2752Crossref PubMed Scopus (4823) Google Scholar), were carried out as described previously (27.Chen S.R. Leong P. Imredy J.P. Bartlett C. Zhang L. MacLennan D.H. Biophys. J. 1997; 73: 1904-1912Abstract Full Text PDF PubMed Scopus (33) Google Scholar). A microfluorometry system (Photon Technologies Inc.) was used to monitor the Fura-2 AM fluorescence changes in transiently transfected or nontransfected HEK-293 cells, as described previously (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). Transfected HEK-293 cells grown in 100-mm Petri dishes were solubilized with 1% CHAPS and 5 mg/ml phosphatidylcholine and analyzed with the [3H]ryanodine binding assay described previously (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). In brief, 25-μl aliquots of solubilized total cellular protein were diluted 10-fold in binding buffer composed of 0.5 m KCl, 1 mm ATP, 100 μm free Ca2+, 0.2 mm EGTA, 50 mm Hepes, pH 7.1, a protease inhibitor mix (0.1 mm AEBSF, 1 mm benzamidine, 1 μg/ml of each of leupeptin, pepstatin, aprotinin, and E64), and various concentrations of [3H]ryanodine. Nonspecific binding was determined using a 1000-fold excess of unlabeled ryanodine. After 2 h at 37 °C, the 0.25-ml samples were diluted with 1 ml of ice-cold washing buffer composed of 25 mm Hepes, pH 7.1, and 0.25 m KCl and placed on Whatman GF/B membrane filters presoaked with 1% polyethyleneimine in washing buffer. Filters were washed three times with 6 ml of washing buffer. [3H]Ryanodine bound to the filter was quantified by liquid scintillation counting. All binding assays were carried out in duplicate. To assess the effects of Ca2+ on high affinity ryanodine binding, protocols were modified by removal of ATP from the binding buffer and addition of different concentrations of Ca2+ with 2.5 nm [3H]ryanodine. Free Ca2+ was calculated using the apparent binding constants described by Fabiato (29.Fabiato A. Methods Enzymol. 1988; 157: 378-417Crossref PubMed Scopus (977) Google Scholar). About 50 μg of proteins from cells lysed with CHAPS were separated by 5% SDS-polyacrylamide gel electrophoresis (30.Laemmli U.K. Nature. 1970; 227: 680-685Crossref PubMed Scopus (207479) Google Scholar). RyR proteins were detected by immunoblotting (31.Towbin H. Staehelin T. Gordon J. Proc. Natl. Acad. Sci. U. S. A. 1979; 76: 4350-4354Crossref PubMed Scopus (44935) Google Scholar) as described previously (26.Chen S.R. Vaughan D.M. Airey J.A. Coronado R. MacLennan D.H. Biochemistry. 1993; 32: 3743-3753Crossref PubMed Scopus (49) Google Scholar). Protein concentration was determined by dye binding using bovine serum albumin as a standard (32.Lowry O.H. Rosebrough N.J. Farr A.L. Randall R.J. J. Biol. Chem. 1951; 193: 265-275Abstract Full Text PDF PubMed Google Scholar). Data were analyzed with Microcal Origin software (Microcal Software Ltd., Northampton, MA). Scatchard analysis was used to determine the dissociation constant (K d) and maximal binding capacity (B max) from equilibrium binding data. EC50 or IC50 values were obtained by fitting the curves with an equation for logistic dose response. Data are expressed as mean ± S.E. An unpaired Studentt test was used for evaluation of the mean values between groups. A value of p ≤ 0.05 was considered to be statistically significant. In earlier studies, we showed differences in the curves of Ca2+ dependence for [3H]ryanodine binding to recombinant RyR1 and RyR2 that could be equated with differences in Ca2+ inactivation (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). We exploited this difference to locate the Ca2+ inactivation sites of RyR1 in the COOH terminus, in particular, in fragments between amino acids 3726–4186 (F9) and 4187–4628 (F10) (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar). We also observed that chimeras containing F9 and F10 were more sensitive to Ca2+ and caffeine activation, implying that these two sequences might be involved in Ca2+ and caffeine activation of RyR. Because F10 encompasses the most divergent region (D1) between RyR1 and RyR2, and because we were interested in the role of this region in Ca2+ release channel function, we subdivided the D1 region of RyR1 into three smaller chimeras by substitution with the corresponding regions of RyR2 (Fig. 1). We also constructed a deletion mutant in the D1 region of RyR1 (Fig.1). These constructs were then transiently expressed in HEK-293 cells, and the Ca2+ dependence of [3H]ryanodine binding (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar) was used as an indirect measurement of Ca2+activation and inactivation. In addition, caffeine-induced Ca2+ release was measured in vivo by Ca2+ photometry using HEK-293 cells transfected with chimeric or deletion-mutant RYR cDNA. Immunostaining of CHAPS-solubilized cell lysates, using monoclonal antibody 34C against an epitope located in RyR1 amino acids 2756–2803 (11.Tong J. Oyamada H. Demaurex N. Grinstein S. McCarthy T.V. MacLennan D.H. J. Biol. Chem. 1997; 272: 26332-26339Abstract Full Text Full Text PDF PubMed Scopus (219) Google Scholar), was used to detect the expression of RyR proteins in transfected HEK-293 cells. Fig. 2 shows the absence of RyR immunostaining in pcDNA-transfected cells. Because the chimeric and deletion-mutated proteins all retained the RyR1 epitope, immunostaining with monoclonal antibody 34C was used as a measure of RyR expression. Immunostaining demonstrated that the chimeras RF10a, RF10b, RF10c, and the deletion mutant Δ4274–4535-R1 were expressed at levels comparable to wild type RyR1 and that RF10a and Δ4274–4535-R1 were expressed at levels higher than that of RyR1. It has been demonstrated that RyR1 and RyR2 are not expressed with equal efficiency, and F10 and F11 from RyR2 have been shown to be the key sequences conferring high levels of expression in HEK-293 cells (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar). In this study, a smaller fragment, F10a from RyR2, was also shown to confer high level expression. We have observed that RyR2 and chimeras containing the F10 sequence have a higher mobility than RyR1 in SDS gels. When F10 was subdivided, there was no obvious difference in mobility when compared with RyR1. The mutant Δ4274–4535-R1 had a slightly higher mobility due to deletion of 262 amino acids. We used Fura-2 fluorescence to measure the properties of caffeine-induced Ca2+ release in the chimeric or deletion-mutant proteins expressed in HEK-293 cells (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). No significant Ca2+ release occurred with caffeine up to 30 mm in pcDNA-transfected cells (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar), but caffeine-induced Ca2+ release was observed in cells transfected with each of the constructs. The peak fluorescence amplitude was measured during the course of incremental application of 0.03 to 30 mm caffeine and normalized to the peak amplitude for maximal Ca2+ release induced by 30 mmcaffeine. EC50 values were then calculated by fitting the caffeine dose-response curves with an equation for logistic dose response. As described previously (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar), EC50 values measuring the caffeine sensitivity of Ca2+ release were higher for recombinant RyR2 than for recombinant RyR1 (Fig.3). Dose response curves for RyR1, RyR2, RF10, and chimeric and deletion-mutant proteins are shown in Fig. 3. EC50 values are summarized in the inset to Fig.3. Chimera RF10b had an EC50 value for caffeine that was similar to RyR1. However, chimeras RF10, RF10a and mutant Δ4274–4535-R1 had lower caffeine EC50 values, and RF10c had a higher caffeine EC50 value. Because caffeine activation was retained after deletion of sequences in the D1 region, caffeine activation sites are unlikely to be located in this region. The higher caffeine sensitivity exhibited by the RF10 and RF10a chimeras and the deletion mutant Δ4274–4535-R1 and the lower sensitivity by the RF10c chimera might be explained by induced conformations that modulate the Ca2+ release channel function, either negatively or positively. The Hill coefficients for chimeras RF10, RF10a, and RF10b resembled that for RyR2 (Fig. 3,inset), and the Hill coefficients for other constructs were similar to that of RyR1, indicating that the F10a (and possibly F10b) sequence of RyR2 can partially suppress the co-operative interactions that occur in RyR1. We measured the equilibrium binding properties of [3H]ryanodine to the chimeric and mutated RyR proteins to determine whether the high affinity ryanodine binding site was preserved. We also used [3H]ryanodine binding to determine expression levels, because 1 mol of a tetrameric RyR molecule binds 1 mol of ryanodine with high affinity (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar, 26.Chen S.R. Vaughan D.M. Airey J.A. Coronado R. MacLennan D.H. Biochemistry. 1993; 32: 3743-3753Crossref PubMed Scopus (49) Google Scholar, 33.Inui M. Saito A. Fleischer S. J. Biol. Chem. 1987; 262: 1740-1747Abstract Full Text PDF PubMed Google Scholar). Scatchard analysis showed a single binding site in all of the chimeras and the deletion mutant (Fig. 4), effectively ruling out the possibility that the high affinity ryanodine binding site is located between amino acids 4274 and 4535.K d values for these mutants were similar to those for wild type RyR2 and RyR1 (Ref. 21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar and Fig. 4, inset), ranging from 1.6 nm for RF10 to 3.3 nm for RF10a. These data indicate that the high affinity binding site for ryanodine is unchanged in all of these mutants. TheB max values ranged from 0.22 to 1.42 pmol/mg of protein in these chimeras and mutants (Fig. 4, inset), reflecting different expression levels. These results show that RF10a expression, like RF10 expression, was increased 5–6-fold over RyR1 expression. The expression of Δ4274–4535-R1 was increased 3-fold, confirming results from immunoblotting. As reported previously (21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar), there was no significant binding in lysates isolated from pcDNA-transfected HEK-293 cells. Dose-response curves for Ca2+ activation and inactivation of [3H]ryanodine binding to wild type RyR1 and RyR2 and RF10 were shown previously (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar, 21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). The dose-response curves for Ca2+ activation and inactivation of [3H]ryanodine binding to the mutants are shown in Fig.5, where they are compared with those of RF10. At Ca2+ concentrations below pCa 7, there was little binding of [3H]ryanodine to the RyR proteins, except for Δ4274–4535-R1, which bound nearly 0.14 pmol of [3H]ryanodine per mg of protein in the absence of Ca2+. This level of activation was observed even in the presence of 1 mm EGTA (data not shown). As with wild type RyR1 and RyR2, [3H]ryanodine binding was activated by increasing Ca2+ concentrations, with maximal binding occurring between pCa 5.7 and pCa 4 for most of the constructs. EC50 values, expressed in pCa units, were similar for wild type RyR1 and RyR2, and the EC50 value for RF10a did not differ from that of either RyR1 or RyR2 (Fig. 5 and TableI). However, in chimeras RF10b and RF10c and especially in the mutant Δ4274–4535-R1, activation of [3H]ryanodine binding was observed with lower Ca2+ concentrations, and EC50 values were significantly higher than those for wild type RyR1 and RyR2 (Fig. 5 and Table I). In addition, the slope for RF10b and Δ4274–4535-R1 was decreased below 0.7, indicating that the co-operativity of Ca2+ activation that is observed in wild type RyR1 and RyR2 is absent in these mutants and is lowered below 2 in mutants RF10 and RF10c.Table ICa 2+ activation (EC50) and Ca 2+ inactivation (IC50) for Ca 2+ dependence of [ 3 H]ryanodine binding to RyR1, RyR2, and their chimeras and mutantEC50SlopeIC50SlopepCapCaRyR16.21 ± 0.082.2 ± 0.62.43 ± 0.131.1 ± 0.2RyR26.14 ± 0.122.6 ± 0.30.12 ± 0.08ap ≤ 0.05 compared with RyR1.0.2 ± 0.1RF106.70 ± 0.05ap ≤ 0.05 compared with RyR1.1.6 ± 0.11.56 ± 0.09ap ≤ 0.05 compared with RyR1.1.1 ± 0.3RF10a6.49 ± 0.172.0 ± 0.11.90 ± 0.03ap ≤ 0.05 compared with RyR1.1.1 ± 0.3RF10b6.86 ± 0.09ap ≤ 0.05 compared with RyR1.0.7 ± 0.12.09 ± 0.110.9 ± 0.1RF10c6.51 ± 0.03ap ≤ 0.05 compared with RyR1.1.3 ± 0.22.20 ± 0.081.0 ± 0.1Δ4274–4535-R17.45 ± 0.08ap ≤ 0.05 compared with RyR1.0.5 ± 0.022.01 ± 0.09ap ≤ 0.05 compared with RyR1.1.5 ± 0.2Values were obtained by fitting the curves, from three or four separate experiments for each construct, as shown in Fig. 5, with the logistic dose response equation for EC50 and IC50. Data for RyR1, RyR2, and RF10 have been shown elsewhere (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar, 21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar).a p ≤ 0.05 compared with RyR1. Open table in a new tab Values were obtained by fitting the curves, from three or four separate experiments for each construct, as shown in Fig. 5, with the logistic dose response equation for EC50 and IC50. Data for RyR1, RyR2, and RF10 have been shown elsewhere (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar, 21.Du G.G. Imredy J.P. MacLennan D.H. J. Biol. Chem. 1998; 273: 33259-33266Abstract Full Text Full Text PDF PubMed Scopus (47) Google Scholar). Ca2+ inactivation was studied indirectly through measurement of the inhibition of [3H]ryanodine binding by elevated Ca2+. IC50 values for the chimeric and deletion mutant RyR proteins, expressed in pCa units, are also presented in Table I and illustrated in Fig. 5, where they are compared with values for wild type RyR1, RyR2, and RF10. The IC50for chimeras RF10b and F10c did not differ from that of wild type RyR1. IC50 values were significantly reduced, however, for chimera RF10a (pCa 1.90), associating the RF10a sequence (amino acids 4187–4381) with the low affinity Ca2+ binding site. The slopes for the curves of inactivation were not changed for chimeras and mutant Δ4274–4535-R1, when compared with RyR1. Mutant Δ4274–4535-R1 also had a lower IC50 (pCa 2.01) when compared with RyR1, suggesting some involvement of this sequence with the low affinity Ca2+ binding site, perhaps in its region of overlap with RF10a. In an earlier study, we measured alterations in Ca2+release channel function that resulted from exchange of RyR1 sequences with corresponding sequences in RyR2 (19.Du G.G. MacLennan D.H. J. Biol. Chem. 1999; 274: 26120-26126Abstract Full Text Full Text PDF PubMed Scopus (54) Google Scholar). In this study, we substituted shorter sequences in RyR1 with corresponding sequences in RyR2 and measured alterations in channel sensitivity to Ca2+ and caffeine. This strategy allowed us to map part of the Ca2+ inactivation site to the F10a sequence (amino acids 4187–4381). Decreased sensitivity to inactivation by Ca2+ in mutant Δ4274–4535-R1 may be due either to the deletion of the Ca2+ inactivation site in the region of overlap with RF10a or to conformational changes induced by the deletion. Increased sensitivity to activation by Ca2+ or caffeine was also observed in all chimeras. This is unlikely to involve the activation sites for Ca2+ and caffeine, because the deletion mutant Δ4274–4535-R1 exhibited increased sensitivity to Ca2+ and caffeine. In fact, high affinity Ca2+binding sites are suggested to be in hydrophobic sequences (14.Chen S.R.W. Ebisawa K. Li X. Zhang L. J. Biol. Chem. 1998; 273: 14675-14678Abstract Full Text Full Text PDF PubMed Scopus (108) Google Scholar, 15.Du G.G. MacLennan D.H. J. Biol. Chem. 1998; 273: 31867-31872Abstract Full Text Full Text PDF PubMed Scopus (53) Google Scholar,34.Feng W. Shoshan-Barmatz V. Mol. Membr. Biol. 1996; 13: 85-93Crossref PubMed Scopus (9) Google Scholar), which were not analyzed in this study. Chen et al. (14.Chen S.R.W. Ebisawa K. Li X. Zhang L. J. Biol. Chem. 1998; 273: 14675-14678Abstract Full Text Full Text PDF PubMed Scopus (108) Google Scholar) have presented evidence for a Ca2+ activation site involving a residue in RyR3 that corresponds to Glu4032, located in TM2 of RyR1. They showed that the mutant channel retained normal conductance, but sensitivity to activating Ca2+ was reduced by 10,000-fold, and heterotetrameric forms of wild type and mutant channels, created by coexpression, had intermediate Ca2+ sensitivities. Therefore, it is most likely that the increased sensitivity observed in the chimeras was due to conformational changes that might have altered function through long range effects. The use of antibodies against several domains in the D1 region has been associated with Ca2+ activation of the Ca2+release channel. Polyclonal antibodies against the junctional face membrane of skeletal muscle sarcoplasmic reticulum and purified ryanodine receptor from skeletal muscle blocked Ca2+-induced Ca2+ release and decreased single channel open probability and conductance (35.Zorzato F. Chu A. Volpe P. Biochem. J. 1989; 261: 863-870Crossref PubMed Scopus (14) Google Scholar, 36.Fill M. Mejia-Alvarez R. Zorzato F. Volpe P. Stefani E. Biochem. J. 1991; 273: 449-457Crossref PubMed Scopus (26) Google Scholar). Some of the epitopes recognized by these anti-RyR antibodies have been mapped to amino acids 4445–4586 and 4760–4877. Polyclonal antibodies raised against amino acids 4380–4621 and 4425–4621 in the C terminus of RyR1 decreased Ca2+-induced Ca2+ release and doxorubicin-induced Ca2+ release from isolated terminal cisternae (37.Treves S. Chiozzi P. Zorzato F. Biochem. J. 1993; 291: 757-763Crossref PubMed Scopus (26) Google Scholar). An antibody raised against amino acids 4478–4512 increased the Ca2+ sensitivity of the Ca2+release channel (38.Chen S.R. Zhang L. MacLennan D.H. J. Biol. Chem. 1992; 267: 23318-23326Abstract Full Text PDF PubMed Google Scholar). After the antibody was purified with a Pro-Glu repeat peptide sequence (amino acids 4490–4499), the purified antibody inhibited Ca2+- or caffeine-activated channel activity but did not inhibit ATP-activated channel activity (39.Chen S.R. Zhang L. MacLennan D.H. J. Biol. Chem. 1993; 268: 13414-13421Abstract Full Text PDF PubMed Google Scholar). The major epitopes for the antibody made against amino acids 4478–4512 were shown not to be located in the Pro-Glu repeat region. From these results, it might be deduced that the domains involving Ca2+ activation are associated with or lie close to the cytoplasmic loop between proposed transmembrane sequences 2 and 5 in the Zorzato numbering scheme. These earlier results are, therefore, consistent with our current results. However, the Ca2+ activation site itself is not likely to be located between amino acids 4274 and 4535, as discussed above. Because deletion of amino acids 4274–4535 increased channel sensitivity to activation by Ca2+ and caffeine, this sequence in RyR1 could form a complex domain, which modulates RyR1 channel function, presumably by suppressing channel activation. The sequences in RyR1 that were exchanged or deleted in this study, with the exception of RF10c, would be likely to form a cytoplasmic loop between M2 and M5 in the topological model of Zorzato et al.(7.Zorzato F. Fujii J. Otsu K. Phillips M. Green N.M. Lai F.A. Meissner G. MacLennan D.H. J. Biol. Chem. 1990; 265: 2244-2256Abstract Full Text PDF PubMed Google Scholar). Although M3 (amino acids 4277–4299), M4 (amino acids 4342–4362), and M5 (amino acids 4559–4580) were predicted to be transmembrane sequences (7.Zorzato F. Fujii J. Otsu K. Phillips M. Green N.M. Lai F.A. Meissner G. MacLennan D.H. J. Biol. Chem. 1990; 265: 2244-2256Abstract Full Text PDF PubMed Google Scholar), M3 and M4 are not highly conserved among RyR isoforms and are no longer considered to be transmembrane sequences (15.Du G.G. MacLennan D.H. J. Biol. Chem. 1998; 273: 31867-31872Abstract Full Text Full Text PDF PubMed Scopus (53) Google Scholar). M5 is one of the most hydrophobic sequences in RyR1 and is almost certain to be a transmembrane sequence (6.Takeshima H. Nishimura S. Matsumoto T. Ishida H. Kangawa K. Minamino N. Matsuo H. Ueda M. Hanaoka M. Hirose T. Numa S. Nature. 1989; 339: 439-445Crossref PubMed Scopus (868) Google Scholar, 7.Zorzato F. Fujii J. Otsu K. Phillips M. Green N.M. Lai F.A. Meissner G. MacLennan D.H. J. Biol. Chem. 1990; 265: 2244-2256Abstract Full Text PDF PubMed Google Scholar). The fact that channel function was not destroyed after deletion of amino acids 4274–4535, which includes the sequences formerly designated M3 and M4, provides further reason to think that these sequences do not form any part of the channel pore. Nothing is known of the structure of this probable cytoplasmic loop region, although Gly-, Ala-, and Pro-rich sequences between amino acids 4274 and 4535 (6.Takeshima H. Nishimura S. Matsumoto T. Ishida H. Kangawa K. Minamino N. Matsuo H. Ueda M. Hanaoka M. Hirose T. Numa S. Nature. 1989; 339: 439-445Crossref PubMed Scopus (868) Google Scholar, 7.Zorzato F. Fujii J. Otsu K. Phillips M. Green N.M. Lai F.A. Meissner G. MacLennan D.H. J. Biol. Chem. 1990; 265: 2244-2256Abstract Full Text PDF PubMed Google Scholar) might limit the extent of helical structure. Most of this sequence is hydrophilic, implying that at least part of the sequence is surface-exposed and antigenic (39.Chen S.R. Zhang L. MacLennan D.H. J. Biol. Chem. 1993; 268: 13414-13421Abstract Full Text PDF PubMed Google Scholar, 40.Marks A.R. Fleischer S. Tempst P. J. Biol. Chem. 1990; 265: 13143-13149Abstract Full Text PDF PubMed Google Scholar). Binding domains for several peptides have been mapped on RyR1 by cryoelectron microscopy (41.Samso M. Trujillo R. Gurrola G.B. Valdivia H.H. Wagenknecht T. J. Cell Biol. 1999; 146: 493-499Crossref PubMed Scopus (63) Google Scholar, 42.Wagenknecht T. Grassucci R. Berkowitz J. Wiederrecht G.J. Xin H.B. Fleischer S. Biophys. J. 1996; 70: 1709-1715Abstract Full Text PDF PubMed Scopus (64) Google Scholar, 43.Wagenknecht T. Radermacher M. Grassucci R. Berkowitz J. Xin H.B. Fleischer S. J. Biol. Chem. 1997; 272: 32463-32471Abstract Full Text Full Text PDF PubMed Scopus (151) Google Scholar). Binding sites for calmodulin have been identified between structural domains 3 and 7 in RyR1 (42.Wagenknecht T. Grassucci R. Berkowitz J. Wiederrecht G.J. Xin H.B. Fleischer S. Biophys. J. 1996; 70: 1709-1715Abstract Full Text PDF PubMed Scopus (64) Google Scholar, 43.Wagenknecht T. Radermacher M. Grassucci R. Berkowitz J. Xin H.B. Fleischer S. J. Biol. Chem. 1997; 272: 32463-32471Abstract Full Text Full Text PDF PubMed Scopus (151) Google Scholar), and calmodulin binding sites have been localized to amino acids 4303–4328 and 4534–4552 (44.Chen S.R. MacLennan D.H. J. Biol. Chem. 1994; 269: 22698-22704Abstract Full Text PDF PubMed Google Scholar,45.Menegazzi P. Larini F. Treves S. Guerrini R. Quadroni M. Zorzato F. Biochemistry. 1994; 33: 9078-9084Crossref PubMed Scopus (71) Google Scholar), which lie in the D1 region. Thus, it is possible that the D1 region lies near structural domain 3 and 7. Deletion of more than 200 amino acids might be apparent in cryoelectron microscopy of this mutant form of RyR1, providing a means to localize it in RyR1. Knowledge of the location of regulatory domains relative to the channel forming domains could be helpful in understanding the regulation of the Ca2+release channel." @default.
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• W2085191605 title "Mutation of Divergent Region 1 Alters Caffeine and Ca2+ Sensitivity of the Skeletal Muscle Ca2+Release Channel (Ryanodine Receptor)" @default.
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