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• W2085192418 endingPage "533" @default.
• W2085192418 startingPage "526" @default.
• W2085192418 abstract "Reaction between NO(*) and manganese tetrakis(N-ethylpyridinium-2-yl)porphyrin (Mn(III)TE-2-PyP(5+)) was investigated at 25 degrees C. At high excess of NO(*) (1.5 mM) the reaction with the oxidized, air-stable form Mn(III)TE-2-PyP(5+) (5 microM), proceeds very slowly (t(1/2) congruent with 60 min). The presence of excess ascorbate (1 mM) produces the reduced form, Mn(II)TE-2-PyP(4+), which reacts with NO(*) stoichiometrically and in the time of mixing (k congruent with 1 x 10(6) M(-1) s(-1)). The high rate of formation and the stability of the product, Mn(II)TE-2-PyP(NO)(4+) (¿Mn(NO)¿(6)), make the reaction outcompete the reaction of NO(*) with O(2). Our in vitro measurements show a linear absorbance response upon addition of NO to a PBS, pH 7.4, solution containing an excess of ascorbate over Mn(III)TE-2-PyP(5+). Thus, the observed interactions can be the basis of a convenient and sensitive spectrophotometric assay for NO(*). Also, it may have important implications for the in vivo behavior of Mn(III)TE-2-PyP(5+) which is currently exploited as a possible therapeutic agent for various oxygen-radical related disorders." @default.
• W2085192418 created "2016-06-24" @default.
• W2085192418 creator A5040227179 @default.
• W2085192418 creator A5087698229 @default.
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• W2085192418 date "2000-10-01" @default.
• W2085192418 modified "2023-10-17" @default.
• W2085192418 title "Nitrosylation of Manganese(II) Tetrakis(N-ethylpyridinium-2-yl)porphyrin: A Simple and Sensitive Spectrophotometric Assay for Nitric Oxide" @default.
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• W2085192418 doi "https://doi.org/10.1006/niox.2000.0303" @default.
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