FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085193432> ?p ?o ?g. }

• W2085193432 endingPage "39" @default.
• W2085193432 startingPage "29" @default.
• W2085193432 abstract "The superoxide anion-generating effect of celecoxib (4-[5-(4-methylpheny)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide); SC58633), a selective cyclooxygenase-2 inhibitor, on human neutrophils was evaluated in this study. Celecoxib induced superoxide anion generation in a concentration-dependent manner in human neutrophils. The EC50 value of celecoxib on superoxide anion generation was 15.5+/-2.5 microM. A NADPH oxidase inhibitor, diphenyliodonium (20 microM), and superoxide dismutase (150 U/ml) completely inhibited the free radical generation caused by celecoxib, indicating that the respiratory burst was activated by celecoxib. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA/AM;10 microM) and staurosporine (200 nM) completely inhibited the superoxide anion release caused by celecoxib, respectively. These data indicated that celecoxib increased superoxide anion release by increasing intracellular calcium and protein kinase C activation. Moreover, 12-(2-cyanoethyl)-6,7,12,13-tetrahydro-13-methyl-5-oxo-5H-indolo(2,3-a)pyrrolo(3,4-C)-carbazole (Go-6976; 1 microM) and 3-[1-[3-(amidinothio)propyl-1H-indol-3-yl]-3-(1-methyl-1H-indol-3-yl)maleimide, methane sulfate (Ro-31-8220; 0.5 microM), specific inhibitors of conventional protein kinase C isotypes (alpha, beta(I) and beta(II)), significantly inhibited superoxide anion release caused by celecoxib. Rottlerin (5 microM), a protein kinase C delta inhibitor, did not affect the free radical generation caused by celecoxib. Celecoxib caused translocation of protein kinase C alpha, beta(I) and beta(II) from the cytosol to the cellular membrane. 2-[2-amino-3-methoxyphenyl]-4H-1-benzopyran-4-one (PD98059; 20 microM) and wortmannin (100 nM) did not decrease the superoxide anion generation caused by celecoxib, indicating that Mitogen-activated protein (MAP) kinase and phosphatidylinositol 3-kinase (PI3 kinase) were not involved in the respiratory burst induced by celecoxib. Pertussis toxin (2 microg/ml), a Gi-protein sensitive inhibitor, significantly inhibited superoxide anion release. Moreover, pertussis toxin significantly inhibited intracellular calcium mobilization and protein kinase C alpha, beta(I) and beta(II) translocation from the cytosol to the membrane. Celecoxib increased beta(2)-integrin expression on human neutrophils and this effect was inhibited by BAPTA/AM (10 microM), superoxide dismutase (150 U/ml), genistein (25 microM) and PD98059 (20 microM). This information indicated that intracellular calcium, superoxide anion, tyrosine kinase and MAP kinase are involved in beta(2)-integrin expression. Furthermore, BAPTA/AM, superoxide dismutase and genistein inhibited celecoxib-increased MAP kinase activity, indicating that MAP kinase is a downstream signal for beta(2)-integrin expression. In conclusion, celecoxib stimulates superoxide anion release from human neutrophils by activating pertussis toxin sensitive G-protein. An increase in intracellular calcium and protein kinase C alpha, beta(I) and beta(II) is involved in this process. Celecoxib also regulates beta(2)-integrin expression through superoxide anion release, tyrosine kinase and p42/p44 MAP kinase on human neutrophils." @default.
• W2085193432 created "2016-06-24" @default.
• W2085193432 creator A5006768945 @default.
• W2085193432 creator A5035126071 @default.
• W2085193432 creator A5054878361 @default.
• W2085193432 date "2004-01-01" @default.
• W2085193432 modified "2023-10-10" @default.
• W2085193432 title "Celecoxib simulates respiratory burst through pertussis toxin-sensitive G-protein, a possible signal for β2-integrin expression on human neutrophils" @default.
• W2085193432 cites W139281516 @default.
• W2085193432 cites W148785554 @default.
• W2085193432 cites W1494669487 @default.
• W2085193432 cites W1501012449 @default.
• W2085193432 cites W1527115904 @default.
• W2085193432 cites W1576671418 @default.
• W2085193432 cites W1581317022 @default.
• W2085193432 cites W1604551367 @default.
• W2085193432 cites W1867471385 @default.
• W2085193432 cites W1965980035 @default.
• W2085193432 cites W1967270772 @default.
• W2085193432 cites W1968249471 @default.
• W2085193432 cites W1998394615 @default.
• W2085193432 cites W2017455665 @default.
• W2085193432 cites W2033498682 @default.
• W2085193432 cites W2045191482 @default.
• W2085193432 cites W2058137293 @default.
• W2085193432 cites W2060426330 @default.
• W2085193432 cites W2062270939 @default.
• W2085193432 cites W2069249495 @default.
• W2085193432 cites W2070370694 @default.
• W2085193432 cites W2070620156 @default.
• W2085193432 cites W2074781030 @default.
• W2085193432 cites W2087762274 @default.
• W2085193432 cites W2091011623 @default.
• W2085193432 cites W2100837269 @default.
• W2085193432 cites W2101108802 @default.
• W2085193432 cites W2146611543 @default.
• W2085193432 cites W2151324279 @default.
• W2085193432 cites W2155996040 @default.
• W2085193432 cites W2160773533 @default.
• W2085193432 cites W2178558243 @default.
• W2085193432 cites W2188751783 @default.
• W2085193432 cites W2400264979 @default.
• W2085193432 cites W4237295669 @default.
• W2085193432 cites W4313308248 @default.
• W2085193432 doi "https://doi.org/10.1016/j.ejphar.2003.10.054" @default.
• W2085193432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14729379" @default.
• W2085193432 hasPublicationYear "2004" @default.
• W2085193432 type Work @default.
• W2085193432 sameAs 2085193432 @default.
• W2085193432 citedByCount "19" @default.
• W2085193432 countsByYear W20851934322012 @default.
• W2085193432 countsByYear W20851934322015 @default.
• W2085193432 countsByYear W20851934322016 @default.
• W2085193432 countsByYear W20851934322018 @default.
• W2085193432 countsByYear W20851934322020 @default.
• W2085193432 crossrefType "journal-article" @default.
• W2085193432 hasAuthorship W2085193432A5006768945 @default.
• W2085193432 hasAuthorship W2085193432A5035126071 @default.
• W2085193432 hasAuthorship W2085193432A5054878361 @default.
• W2085193432 hasConcept C181199279 @default.
• W2085193432 hasConcept C184235292 @default.
• W2085193432 hasConcept C185592680 @default.
• W2085193432 hasConcept C195794163 @default.
• W2085193432 hasConcept C2775838275 @default.
• W2085193432 hasConcept C2776151105 @default.
• W2085193432 hasConcept C2776467144 @default.
• W2085193432 hasConcept C2778769476 @default.
• W2085193432 hasConcept C2779084600 @default.
• W2085193432 hasConcept C2779533339 @default.
• W2085193432 hasConcept C2779719074 @default.
• W2085193432 hasConcept C2780795997 @default.
• W2085193432 hasConcept C41625074 @default.
• W2085193432 hasConcept C48349386 @default.
• W2085193432 hasConcept C49773422 @default.
• W2085193432 hasConcept C55493867 @default.
• W2085193432 hasConcept C62478195 @default.
• W2085193432 hasConcept C80631254 @default.
• W2085193432 hasConceptScore W2085193432C181199279 @default.
• W2085193432 hasConceptScore W2085193432C184235292 @default.
• W2085193432 hasConceptScore W2085193432C185592680 @default.
• W2085193432 hasConceptScore W2085193432C195794163 @default.
• W2085193432 hasConceptScore W2085193432C2775838275 @default.
• W2085193432 hasConceptScore W2085193432C2776151105 @default.
• W2085193432 hasConceptScore W2085193432C2776467144 @default.
• W2085193432 hasConceptScore W2085193432C2778769476 @default.
• W2085193432 hasConceptScore W2085193432C2779084600 @default.
• W2085193432 hasConceptScore W2085193432C2779533339 @default.
• W2085193432 hasConceptScore W2085193432C2779719074 @default.
• W2085193432 hasConceptScore W2085193432C2780795997 @default.
• W2085193432 hasConceptScore W2085193432C41625074 @default.
• W2085193432 hasConceptScore W2085193432C48349386 @default.
• W2085193432 hasConceptScore W2085193432C49773422 @default.
• W2085193432 hasConceptScore W2085193432C55493867 @default.
• W2085193432 hasConceptScore W2085193432C62478195 @default.
• W2085193432 hasConceptScore W2085193432C80631254 @default.
• W2085193432 hasIssue "1" @default.
• W2085193432 hasLocation W20851934321 @default.
• W2085193432 hasLocation W20851934322 @default.

• next