Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085206814> ?p ?o ?g. }
- W2085206814 endingPage "257" @default.
- W2085206814 startingPage "243" @default.
- W2085206814 abstract "Extensive biochemical and structural analyses have been performed on the putative DNA repair proteins of hyperthermophilic archaea, in contrast to the few genetic analyses of the genes encoding these proteins. Accordingly, little is known about the repair pathways used by archaeal cells at high temperature. Here, we attempted to disrupt the genes encoding the potential repair proteins in the genome of the hyperthermophilic archaeon Thermococcus kodakaraensis. We succeeded in isolating null mutants of the hjc, hef, hjm, xpb, and xpd genes, but not the radA, rad50, mre11, herA, nurA, and xpg/fen1 genes. Phenotypic analyses of the gene-disrupted strains showed that the xpb and xpd null mutants are only slightly sensitive to ultraviolet (UV) irradiation, methyl methanesulfonate (MMS) and mitomycin C (MMC), as compared with the wild-type strain. The hjm null mutant showed sensitivity specifically to mitomycin C. On the other hand, the null mutants of the hjc gene lacked increasing sensitivity to any type of DNA damage. The Hef protein is particularly important for maintaining genome homeostasis, by functioning in the repair of a wide variety of DNA damage in T. kodakaraensis cells. Deletion of the entire hef gene or of the segments encoding either its nuclease or helicase domain produced similar phenotypes. The high sensitivity of the Δhef mutants to MMC suggests that Hef performs a critical function in the repair process of DNA interstrand cross-links. These damage-sensitivity profiles suggest that the archaeal DNA repair system has processes depending on repair-related proteins different from those of eukaryotic and bacterial DNA repair systems using homologous repair proteins analyzed here." @default.
- W2085206814 created "2016-06-24" @default.
- W2085206814 creator A5006989967 @default.
- W2085206814 creator A5045012147 @default.
- W2085206814 creator A5067107875 @default.
- W2085206814 creator A5091702193 @default.
- W2085206814 date "2010-01-01" @default.
- W2085206814 modified "2023-10-14" @default.
- W2085206814 title "Genetic analysis of DNA repair in the hyperthermophilic archaeon, Thermococcus kodakaraensis" @default.
- W2085206814 cites W1507468227 @default.
- W2085206814 cites W1511719954 @default.
- W2085206814 cites W1567434518 @default.
- W2085206814 cites W1584653951 @default.
- W2085206814 cites W1658765693 @default.
- W2085206814 cites W1971015996 @default.
- W2085206814 cites W1972044819 @default.
- W2085206814 cites W1981680259 @default.
- W2085206814 cites W1981934918 @default.
- W2085206814 cites W1985703657 @default.
- W2085206814 cites W1987615467 @default.
- W2085206814 cites W1988822278 @default.
- W2085206814 cites W1997272092 @default.
- W2085206814 cites W1998239575 @default.
- W2085206814 cites W2000988465 @default.
- W2085206814 cites W2015220743 @default.
- W2085206814 cites W2023336924 @default.
- W2085206814 cites W2027748565 @default.
- W2085206814 cites W2029063714 @default.
- W2085206814 cites W2036099589 @default.
- W2085206814 cites W2040033588 @default.
- W2085206814 cites W2040049093 @default.
- W2085206814 cites W2041022000 @default.
- W2085206814 cites W2049405253 @default.
- W2085206814 cites W2054186158 @default.
- W2085206814 cites W2055532978 @default.
- W2085206814 cites W2059168612 @default.
- W2085206814 cites W2060602368 @default.
- W2085206814 cites W2063036017 @default.
- W2085206814 cites W2063860903 @default.
- W2085206814 cites W2064577894 @default.
- W2085206814 cites W2064853448 @default.
- W2085206814 cites W2066218908 @default.
- W2085206814 cites W2066975479 @default.
- W2085206814 cites W2070266683 @default.
- W2085206814 cites W2074273508 @default.
- W2085206814 cites W2078380152 @default.
- W2085206814 cites W2080380418 @default.
- W2085206814 cites W2087967916 @default.
- W2085206814 cites W2094678250 @default.
- W2085206814 cites W2094958519 @default.
- W2085206814 cites W2098386120 @default.
- W2085206814 cites W2105190461 @default.
- W2085206814 cites W2107090468 @default.
- W2085206814 cites W2111461823 @default.
- W2085206814 cites W2114382972 @default.
- W2085206814 cites W2119940546 @default.
- W2085206814 cites W2121404986 @default.
- W2085206814 cites W2122591225 @default.
- W2085206814 cites W2126110410 @default.
- W2085206814 cites W2130547198 @default.
- W2085206814 cites W2131552409 @default.
- W2085206814 cites W2135077391 @default.
- W2085206814 cites W2140083926 @default.
- W2085206814 cites W2143918999 @default.
- W2085206814 cites W2144926272 @default.
- W2085206814 cites W2145903208 @default.
- W2085206814 cites W2147670977 @default.
- W2085206814 cites W2151488709 @default.
- W2085206814 cites W2157004858 @default.
- W2085206814 cites W2166665085 @default.
- W2085206814 cites W2166865790 @default.
- W2085206814 cites W2222627402 @default.
- W2085206814 doi "https://doi.org/10.1266/ggs.85.243" @default.
- W2085206814 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21178304" @default.
- W2085206814 hasPublicationYear "2010" @default.
- W2085206814 type Work @default.
- W2085206814 sameAs 2085206814 @default.
- W2085206814 citedByCount "69" @default.
- W2085206814 countsByYear W20852068142012 @default.
- W2085206814 countsByYear W20852068142013 @default.
- W2085206814 countsByYear W20852068142014 @default.
- W2085206814 countsByYear W20852068142015 @default.
- W2085206814 countsByYear W20852068142016 @default.
- W2085206814 countsByYear W20852068142017 @default.
- W2085206814 countsByYear W20852068142018 @default.
- W2085206814 countsByYear W20852068142019 @default.
- W2085206814 countsByYear W20852068142020 @default.
- W2085206814 countsByYear W20852068142021 @default.
- W2085206814 countsByYear W20852068142022 @default.
- W2085206814 countsByYear W20852068142023 @default.
- W2085206814 crossrefType "journal-article" @default.
- W2085206814 hasAuthorship W2085206814A5006989967 @default.
- W2085206814 hasAuthorship W2085206814A5045012147 @default.
- W2085206814 hasAuthorship W2085206814A5067107875 @default.
- W2085206814 hasAuthorship W2085206814A5091702193 @default.
- W2085206814 hasBestOaLocation W20852068141 @default.
- W2085206814 hasConcept C104317684 @default.
- W2085206814 hasConcept C104451858 @default.