Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085221310> ?p ?o ?g. }
- W2085221310 endingPage "118" @default.
- W2085221310 startingPage "87" @default.
- W2085221310 abstract "Abstract Many proteins function in the crystalline state, making crystallography a tool that, beside structure, can address mechanism. By initiating biological turnover in the crystal, transient structural species form, which may be filmed ‘on the fly’ by Laue diffraction or captured by trapping methods. These strategies are jointly referred to as ‘kinetic crystallography’. In this article, we review the general concepts of kinetic crystallography in the context of the conformational energy landscape of a protein. Whereas Laue diffraction is best suited to the investigation of cyclic, ultra-fast and light-triggered reactions, trapping approaches, on the other hand, are applicable to a wider range of biological systems but require care to avoid artefacts. Complementary methods – mainly UV/visible single-crystal spectroscopy – have proven essential to design, interpret and validate kinetic crystallography experiments. Achievements in the field as well as remaining puzzling questions are considered through the examination of recently published work: real-time-resolved crystallography of dimeric haemoglobin based on pump-probe Laue diffraction, temperature-trapping crystallography of acetylcholinesterase based on photo- and radio-induced ligand cleavage, and lattice-trapping crystallography of superoxide reductase based on product soaking and the combined use of X-ray diffraction and Raman spectroscopy. Keywords: kinetic crystallographyenzyme mechanismprotein dynamicsmicrospectrophotometrylight-sensitive proteinsintermediate statescaged compoundstime-resolved crystallographytrapping methods Acknowledgements We thank G. Katona for constructive reading of the manuscript and V. Srajer for providing us with Figure 5." @default.
- W2085221310 created "2016-06-24" @default.
- W2085221310 creator A5010878167 @default.
- W2085221310 creator A5073198689 @default.
- W2085221310 date "2009-04-01" @default.
- W2085221310 modified "2023-10-04" @default.
- W2085221310 title "Kinetic protein crystallography: a tool to watch proteins in action" @default.
- W2085221310 cites W1488229693 @default.
- W2085221310 cites W1488760543 @default.
- W2085221310 cites W1500927176 @default.
- W2085221310 cites W1933189132 @default.
- W2085221310 cites W1942533571 @default.
- W2085221310 cites W1965064861 @default.
- W2085221310 cites W1965430869 @default.
- W2085221310 cites W1965789154 @default.
- W2085221310 cites W1966211961 @default.
- W2085221310 cites W1966683963 @default.
- W2085221310 cites W1970589095 @default.
- W2085221310 cites W1973702340 @default.
- W2085221310 cites W1974200376 @default.
- W2085221310 cites W1977258623 @default.
- W2085221310 cites W1977838691 @default.
- W2085221310 cites W1978384854 @default.
- W2085221310 cites W1980202387 @default.
- W2085221310 cites W1980583000 @default.
- W2085221310 cites W1981457114 @default.
- W2085221310 cites W1983860153 @default.
- W2085221310 cites W1986206446 @default.
- W2085221310 cites W1986316905 @default.
- W2085221310 cites W1988122552 @default.
- W2085221310 cites W1991563862 @default.
- W2085221310 cites W1991565523 @default.
- W2085221310 cites W1994249090 @default.
- W2085221310 cites W1995312390 @default.
- W2085221310 cites W1996084127 @default.
- W2085221310 cites W1996086392 @default.
- W2085221310 cites W1997477098 @default.
- W2085221310 cites W2000293919 @default.
- W2085221310 cites W2001804099 @default.
- W2085221310 cites W2003789414 @default.
- W2085221310 cites W2006497452 @default.
- W2085221310 cites W2010393453 @default.
- W2085221310 cites W2011202597 @default.
- W2085221310 cites W2011647421 @default.
- W2085221310 cites W2013947994 @default.
- W2085221310 cites W2014860926 @default.
- W2085221310 cites W2016728553 @default.
- W2085221310 cites W2018535796 @default.
- W2085221310 cites W2021191010 @default.
- W2085221310 cites W2027659453 @default.
- W2085221310 cites W2027955282 @default.
- W2085221310 cites W2028067831 @default.
- W2085221310 cites W2028750260 @default.
- W2085221310 cites W2031492460 @default.
- W2085221310 cites W2032081633 @default.
- W2085221310 cites W2032280563 @default.
- W2085221310 cites W2032328556 @default.
- W2085221310 cites W2033149065 @default.
- W2085221310 cites W2033285121 @default.
- W2085221310 cites W2033685625 @default.
- W2085221310 cites W2034397295 @default.
- W2085221310 cites W2035506170 @default.
- W2085221310 cites W2038418348 @default.
- W2085221310 cites W2040979060 @default.
- W2085221310 cites W2041504837 @default.
- W2085221310 cites W2041860237 @default.
- W2085221310 cites W2043673715 @default.
- W2085221310 cites W2044409792 @default.
- W2085221310 cites W2046002103 @default.
- W2085221310 cites W2047465535 @default.
- W2085221310 cites W2048421180 @default.
- W2085221310 cites W2048822589 @default.
- W2085221310 cites W2048944220 @default.
- W2085221310 cites W2048999806 @default.
- W2085221310 cites W2053927574 @default.
- W2085221310 cites W2063284939 @default.
- W2085221310 cites W2063683697 @default.
- W2085221310 cites W2066864500 @default.
- W2085221310 cites W2069192355 @default.
- W2085221310 cites W2069606630 @default.
- W2085221310 cites W2069775591 @default.
- W2085221310 cites W2072921124 @default.
- W2085221310 cites W2073740862 @default.
- W2085221310 cites W2073879965 @default.
- W2085221310 cites W2075387050 @default.
- W2085221310 cites W2076227184 @default.
- W2085221310 cites W2076265909 @default.
- W2085221310 cites W2078810639 @default.
- W2085221310 cites W2080712985 @default.
- W2085221310 cites W2081176965 @default.
- W2085221310 cites W2083280040 @default.
- W2085221310 cites W2083524802 @default.
- W2085221310 cites W2083670117 @default.
- W2085221310 cites W2085958374 @default.
- W2085221310 cites W2086735239 @default.
- W2085221310 cites W2089015588 @default.
- W2085221310 cites W2090979132 @default.
- W2085221310 cites W2092945631 @default.