FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085242011> ?p ?o ?g. }

• W2085242011 endingPage "472" @default.
• W2085242011 startingPage "464" @default.
• W2085242011 abstract "Transformed and tumoral cells share the characteristic of being able to proliferate even when external calcium concentration is very low. We have investigated whether Human Embryonic Kidney 293 cells, human hepatoma cell Huh-7 and HeLa cells were able to proliferate when kept 72 h in complete culture medium without external calcium. Our data showed that cell proliferation rate was similar over a range of external calcium concentration (2 μM to 1.8 mM). Incubation in the absence of external calcium for 72 h had no significant effect on endoplasmic reticulum (ER) Ca2 + contents but resulted in a significant decrease in cytosolic free calcium concentration in all 3 cell types. Cell proliferation rates were dependent on Orai1 and Orai3 expression levels in HEK293 and HeLa cells. Silencing Orai1 or Orai3 resulted in a 50% reduction in cell proliferation rate. Flow cytometry analysis showed that Orai3 induced a small but significant increase in cell number in G2/M phase. RO-3306, a cdk-1 inhibitor, induced a 90% arrest in G2/M reversible in less than 15 min. Our data showed that progression through G2/M phase after release from RO-3306-induced cell cycle arrest was slower in both Orai1 and Orai3 knock-downs. Overexpressing Orai1, Orai3 and the dominant negative non-permeant mutants E106Q-Orai1 and E81Q-Orai3 induced a 50% increase in cell proliferation rate in HEK293 cells. Our data clearly demonstrated that Orai1 and Orai3 proteins are more important than calcium influx to control cell proliferation in some cell lines and that this process is probably independent of ICRAC and Iarc." @default.
• W2085242011 created "2016-06-24" @default.
• W2085242011 creator A5003776313 @default.
• W2085242011 creator A5021423171 @default.
• W2085242011 creator A5032364845 @default.
• W2085242011 creator A5038200781 @default.
• W2085242011 creator A5048067907 @default.
• W2085242011 creator A5050937351 @default.
• W2085242011 creator A5061202444 @default.
• W2085242011 creator A5075904603 @default.
• W2085242011 date "2014-02-01" @default.
• W2085242011 modified "2023-10-16" @default.
• W2085242011 title "Are Orai1 and Orai3 channels more important than calcium influx for cell proliferation?" @default.
• W2085242011 cites W1510194880 @default.
• W2085242011 cites W1539324781 @default.
• W2085242011 cites W1593868340 @default.
• W2085242011 cites W1597385852 @default.
• W2085242011 cites W1650401181 @default.
• W2085242011 cites W1862817748 @default.
• W2085242011 cites W1963703355 @default.
• W2085242011 cites W1963786607 @default.
• W2085242011 cites W1967127459 @default.
• W2085242011 cites W1968410172 @default.
• W2085242011 cites W1970649592 @default.
• W2085242011 cites W1973482472 @default.
• W2085242011 cites W1976500073 @default.
• W2085242011 cites W1979573280 @default.
• W2085242011 cites W1983522412 @default.
• W2085242011 cites W1991526829 @default.
• W2085242011 cites W1993190159 @default.
• W2085242011 cites W1997113614 @default.
• W2085242011 cites W1997930612 @default.
• W2085242011 cites W2001400243 @default.
• W2085242011 cites W2001501677 @default.
• W2085242011 cites W2010664406 @default.
• W2085242011 cites W2012496178 @default.
• W2085242011 cites W2012579491 @default.
• W2085242011 cites W2017905296 @default.
• W2085242011 cites W2018450524 @default.
• W2085242011 cites W2023164168 @default.
• W2085242011 cites W2025221100 @default.
• W2085242011 cites W2027007192 @default.
• W2085242011 cites W2044076441 @default.
• W2085242011 cites W2055479746 @default.
• W2085242011 cites W2056740334 @default.
• W2085242011 cites W2059989081 @default.
• W2085242011 cites W2072240092 @default.
• W2085242011 cites W2073031672 @default.
• W2085242011 cites W2074307831 @default.
• W2085242011 cites W2077240370 @default.
• W2085242011 cites W2081817263 @default.
• W2085242011 cites W2085032948 @default.
• W2085242011 cites W2091619620 @default.
• W2085242011 cites W2111972883 @default.
• W2085242011 cites W2114291405 @default.
• W2085242011 cites W2118478876 @default.
• W2085242011 cites W2118983117 @default.
• W2085242011 cites W2121255951 @default.
• W2085242011 cites W2121596941 @default.
• W2085242011 cites W2122136617 @default.
• W2085242011 cites W2126692145 @default.
• W2085242011 cites W2134888640 @default.
• W2085242011 cites W2135011583 @default.
• W2085242011 cites W2135324421 @default.
• W2085242011 cites W2137258759 @default.
• W2085242011 cites W2138450406 @default.
• W2085242011 cites W2142333361 @default.
• W2085242011 cites W2144543766 @default.
• W2085242011 cites W2145070342 @default.
• W2085242011 cites W2146066778 @default.
• W2085242011 cites W2146366544 @default.
• W2085242011 cites W2146685564 @default.
• W2085242011 cites W2149375474 @default.
• W2085242011 cites W2158518198 @default.
• W2085242011 cites W2160491587 @default.
• W2085242011 cites W2163191890 @default.
• W2085242011 cites W2163789582 @default.
• W2085242011 cites W2166278863 @default.
• W2085242011 cites W2167663419 @default.
• W2085242011 cites W4252370708 @default.
• W2085242011 doi "https://doi.org/10.1016/j.bbamcr.2013.11.023" @default.
• W2085242011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24321771" @default.
• W2085242011 hasPublicationYear "2014" @default.
• W2085242011 type Work @default.
• W2085242011 sameAs 2085242011 @default.
• W2085242011 citedByCount "27" @default.
• W2085242011 countsByYear W20852420112014 @default.
• W2085242011 countsByYear W20852420112015 @default.
• W2085242011 countsByYear W20852420112016 @default.
• W2085242011 countsByYear W20852420112017 @default.
• W2085242011 countsByYear W20852420112018 @default.
• W2085242011 countsByYear W20852420112020 @default.
• W2085242011 countsByYear W20852420112021 @default.
• W2085242011 countsByYear W20852420112022 @default.
• W2085242011 countsByYear W20852420112023 @default.
• W2085242011 crossrefType "journal-article" @default.
• W2085242011 hasAuthorship W2085242011A5003776313 @default.
• W2085242011 hasAuthorship W2085242011A5021423171 @default.

• next