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- W2085273048 abstract "Selective labeling of the 5′-hydroxyl termini of DNA with polynucleotide kinase has been used to study the number and the identity of the 5′-terminal residues of T7 DNA. The intact strands of native T7 DNA cannot be phosphorylated in the kinase reaction. Prior treatment, however, of the same DNA with phosphatase permits the phosphorylation of the intact strands. The number of nucleotide residues in a single strand of T7 DNA is 4·0 × 104, in agreement with molecular weight estimates by sedimentation and electron microscopy. Only deoxyadenylate and deoxythymidylate are present as 5′-terminal residues of the DNA. In other studies, end-group labeling of polynucleotides has been used to characterize the phosphodiester bond cleavage produced by sonic irradiation of DNA. Sonic irradiation of T7 DNA was found to favor the production of polynucleotides terminated by a 5′-phosphomonoester group." @default.
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- W2085273048 date "1966-01-01" @default.
- W2085273048 modified "2023-10-11" @default.
- W2085273048 title "The 5′-terminal nucleotides of T7 bacteriophage deoxyribonucleic acid" @default.
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- W2085273048 doi "https://doi.org/10.1016/s0022-2836(66)80208-5" @default.
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