FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085273326> ?p ?o ?g. }

• W2085273326 endingPage "558" @default.
• W2085273326 startingPage "552" @default.
• W2085273326 abstract "Generation of reactive oxygen species (ROS) as an early local reaction to muscle crush injury has frequently been predicted. However, although it is known that severe inflammatory reactions occurring after major muscle trauma originate mainly from early local incidents within the injured tissue, no detailed studies exist on the local generation of ROS in response to myocyte destruction thus far. Therefore, in this study, ROS formation after lethal mechanical damage was examined using a model of scraping injury to cultured C2C12 skeletal myocytes and superoxide detection by lucigenin chemiluminescence, nitrotetrazolium blue chloride reduction, or electron spin resonance spectroscopy. Mechanical rupture of myocytes resulted in an immediate release of superoxide from the damaged cells that could be substantially blocked by the superoxide scavengers superoxide dismutase (51%), tiron (95%), and MAMA/NO (93%) and by hypoxia (83% inhibition). Superoxide generation was primarily confined to the myocytes' membrane fraction and 7- to 8-fold enhanced by the addition of NADH or NADPH. The NADPH-enhanced superoxide generation could largely be diminished by the NAD(P)H oxidase inhibitors diphenyleneiodonium and apocynin in cell lysates (97% and 35% inhibition, respectively) and in isolated membrane fractions (61% and 63% inhibition). We thus conclude that immediately after myocyte damage, large amounts of superoxide are formed that predominantly originate from membrane-bound electron-transferring enzymes, especially NAD(P)H oxidase. This suggests a decisive role of ROS in the pathogenesis of tissue trauma, with superoxide being an initiator of the signaling mechanism from injured myocytes to the surrounding tissue and, potentially, to the whole body." @default.
• W2085273326 created "2016-06-24" @default.
• W2085273326 creator A5010954539 @default.
• W2085273326 creator A5015933703 @default.
• W2085273326 creator A5040387308 @default.
• W2085273326 creator A5072963432 @default.
• W2085273326 date "2007-05-01" @default.
• W2085273326 modified "2023-09-26" @default.
• W2085273326 title "DISRUPTION OF SKELETAL MYOCYTES INITIATES SUPEROXIDE RELEASE: CONTRIBUTION OF NAD(P)H OXIDASE" @default.
• W2085273326 cites W1860472154 @default.
• W2085273326 cites W1889843409 @default.
• W2085273326 cites W1967884571 @default.
• W2085273326 cites W1969901996 @default.
• W2085273326 cites W1974614602 @default.
• W2085273326 cites W1975367003 @default.
• W2085273326 cites W1981177875 @default.
• W2085273326 cites W1981921279 @default.
• W2085273326 cites W2001590319 @default.
• W2085273326 cites W2002394849 @default.
• W2085273326 cites W2011964721 @default.
• W2085273326 cites W2019132257 @default.
• W2085273326 cites W2032397591 @default.
• W2085273326 cites W2040599577 @default.
• W2085273326 cites W2042024360 @default.
• W2085273326 cites W2051018869 @default.
• W2085273326 cites W2051416723 @default.
• W2085273326 cites W2061314122 @default.
• W2085273326 cites W2069568986 @default.
• W2085273326 cites W2074290309 @default.
• W2085273326 cites W2074618631 @default.
• W2085273326 cites W2080643714 @default.
• W2085273326 cites W2102619095 @default.
• W2085273326 cites W2104989112 @default.
• W2085273326 cites W2110676635 @default.
• W2085273326 cites W2112634056 @default.
• W2085273326 cites W2120313220 @default.
• W2085273326 cites W2123799914 @default.
• W2085273326 cites W2125817440 @default.
• W2085273326 cites W2129997180 @default.
• W2085273326 cites W2134498505 @default.
• W2085273326 cites W2137825733 @default.
• W2085273326 cites W2138136884 @default.
• W2085273326 cites W2146960356 @default.
• W2085273326 cites W2156696628 @default.
• W2085273326 cites W2162639891 @default.
• W2085273326 cites W2164289223 @default.
• W2085273326 doi "https://doi.org/10.1097/01.shk.0000245027.39483.e4" @default.
• W2085273326 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17438461" @default.
• W2085273326 hasPublicationYear "2007" @default.
• W2085273326 type Work @default.
• W2085273326 sameAs 2085273326 @default.
• W2085273326 citedByCount "27" @default.
• W2085273326 countsByYear W20852733262012 @default.
• W2085273326 countsByYear W20852733262013 @default.
• W2085273326 countsByYear W20852733262014 @default.
• W2085273326 countsByYear W20852733262015 @default.
• W2085273326 countsByYear W20852733262017 @default.
• W2085273326 countsByYear W20852733262018 @default.
• W2085273326 countsByYear W20852733262019 @default.
• W2085273326 countsByYear W20852733262020 @default.
• W2085273326 countsByYear W20852733262021 @default.
• W2085273326 crossrefType "journal-article" @default.
• W2085273326 hasAuthorship W2085273326A5010954539 @default.
• W2085273326 hasAuthorship W2085273326A5015933703 @default.
• W2085273326 hasAuthorship W2085273326A5040387308 @default.
• W2085273326 hasAuthorship W2085273326A5072963432 @default.
• W2085273326 hasBestOaLocation W20852733261 @default.
• W2085273326 hasConcept C12554922 @default.
• W2085273326 hasConcept C134018914 @default.
• W2085273326 hasConcept C153911025 @default.
• W2085273326 hasConcept C181199279 @default.
• W2085273326 hasConcept C185592680 @default.
• W2085273326 hasConcept C207200792 @default.
• W2085273326 hasConcept C2775838275 @default.
• W2085273326 hasConcept C2776151105 @default.
• W2085273326 hasConcept C2777209548 @default.
• W2085273326 hasConcept C2779719074 @default.
• W2085273326 hasConcept C2779959927 @default.
• W2085273326 hasConcept C2780795997 @default.
• W2085273326 hasConcept C2781252958 @default.
• W2085273326 hasConcept C38485361 @default.
• W2085273326 hasConcept C48349386 @default.
• W2085273326 hasConcept C55493867 @default.
• W2085273326 hasConcept C75520062 @default.
• W2085273326 hasConcept C86803240 @default.
• W2085273326 hasConcept C95444343 @default.
• W2085273326 hasConceptScore W2085273326C12554922 @default.
• W2085273326 hasConceptScore W2085273326C134018914 @default.
• W2085273326 hasConceptScore W2085273326C153911025 @default.
• W2085273326 hasConceptScore W2085273326C181199279 @default.
• W2085273326 hasConceptScore W2085273326C185592680 @default.
• W2085273326 hasConceptScore W2085273326C207200792 @default.
• W2085273326 hasConceptScore W2085273326C2775838275 @default.
• W2085273326 hasConceptScore W2085273326C2776151105 @default.
• W2085273326 hasConceptScore W2085273326C2777209548 @default.
• W2085273326 hasConceptScore W2085273326C2779719074 @default.
• W2085273326 hasConceptScore W2085273326C2779959927 @default.
• W2085273326 hasConceptScore W2085273326C2780795997 @default.

• next