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- W2085276093 abstract "Single-stranded nucleic acids from various sources were digested at low temperature with nuclease S1-specific for single-stranded polymers. The order of S1-resistance was : (double-stranded DNA) > poly(A) · poly(U) ⪢ ribosomal RNA, transfer RNA, Qβ RNA, MS2 RNA, TMV RNA > f1 DNA ⪢ heat-denatured DNA ⪢ poly(U). In an experiment using intact f1 DNA and fragmented f1 DNA, the S1 resistance coincided well with the degree of hyperchromicity exhibited by the tested DNA. The S1 resistance was changeable depending on the reaction temperature and the ionic strength in the reaction mixture." @default.
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- W2085276093 date "1972-12-01" @default.
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- W2085276093 title "Estimation of the double-helical content in various single-stranded nucleic acids by treatment with a single strand-specific nuclease" @default.
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- W2085276093 doi "https://doi.org/10.1016/0005-2787(72)90292-4" @default.
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