Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085279970> ?p ?o ?g. }
- W2085279970 endingPage "8112" @default.
- W2085279970 startingPage "8107" @default.
- W2085279970 abstract "The use of non-viral delivery systems has numerous advantages in the area of gene therapy as this approach could circumvent the safety issues that may occur when using viral vectors. Hydrophobically modified chitosan containing five deoxycholic acid groups per 100 anhydroglucose units was synthesized to prepare self-aggregated nanoparticles in aqueous media and utilized to form complexes with plasmid DNA. Formation and various characteristics of DNA/chitosan self-aggregate complexes were investigated by electrophoresis, zeta potential measurement, and dynamic light scattering method. It was hypothesized that complex formation between DNA and chitosan self-aggregates can be strongly dependent on the charge ratio, pH, and incubation time. Migration of DNA on an agarose gel was completely retarded over a charge ratio (+/−) of 4/1 at pH 7.2, and the mean diameter of the complexes was determined to be 350 nm with unimodal size distribution (+/−=4/1; pH 7.2; incubation time, 30 min). The complex formation behavior was also regulated by pH of the media. The incubation time was unlikely to be the critical factor affecting the complex formation behavior due to the instant electrostatic interactions between negatively charged DNA and positively charged chitosan self-aggregates. This approach to controlling the characteristics of DNA/self-aggregate complexes could be critical in the design and tailoring of novel gene delivery systems using non-viral vectors." @default.
- W2085279970 created "2016-06-24" @default.
- W2085279970 creator A5029687263 @default.
- W2085279970 creator A5063454795 @default.
- W2085279970 creator A5073866582 @default.
- W2085279970 creator A5084637239 @default.
- W2085279970 date "2005-09-01" @default.
- W2085279970 modified "2023-09-25" @default.
- W2085279970 title "Complex formation between plasmid DNA and self-aggregates of deoxycholic acid-modified chitosan" @default.
- W2085279970 cites W1560355462 @default.
- W2085279970 cites W1568769949 @default.
- W2085279970 cites W1833064266 @default.
- W2085279970 cites W1968433618 @default.
- W2085279970 cites W1980414709 @default.
- W2085279970 cites W1981434212 @default.
- W2085279970 cites W1985866553 @default.
- W2085279970 cites W1992229594 @default.
- W2085279970 cites W1999373563 @default.
- W2085279970 cites W2004447700 @default.
- W2085279970 cites W2007651161 @default.
- W2085279970 cites W2009482334 @default.
- W2085279970 cites W2010655741 @default.
- W2085279970 cites W2014495927 @default.
- W2085279970 cites W2023104469 @default.
- W2085279970 cites W2028322263 @default.
- W2085279970 cites W2052793555 @default.
- W2085279970 cites W2052917835 @default.
- W2085279970 cites W2060071401 @default.
- W2085279970 cites W2060137851 @default.
- W2085279970 cites W2067886698 @default.
- W2085279970 cites W2070287692 @default.
- W2085279970 cites W2076166680 @default.
- W2085279970 cites W2076862348 @default.
- W2085279970 cites W2077325967 @default.
- W2085279970 cites W2078447684 @default.
- W2085279970 cites W2080225080 @default.
- W2085279970 cites W2083100535 @default.
- W2085279970 doi "https://doi.org/10.1016/j.polymer.2005.06.082" @default.
- W2085279970 hasPublicationYear "2005" @default.
- W2085279970 type Work @default.
- W2085279970 sameAs 2085279970 @default.
- W2085279970 citedByCount "33" @default.
- W2085279970 countsByYear W20852799702012 @default.
- W2085279970 countsByYear W20852799702013 @default.
- W2085279970 countsByYear W20852799702014 @default.
- W2085279970 countsByYear W20852799702015 @default.
- W2085279970 countsByYear W20852799702016 @default.
- W2085279970 countsByYear W20852799702018 @default.
- W2085279970 countsByYear W20852799702020 @default.
- W2085279970 countsByYear W20852799702022 @default.
- W2085279970 crossrefType "journal-article" @default.
- W2085279970 hasAuthorship W2085279970A5029687263 @default.
- W2085279970 hasAuthorship W2085279970A5063454795 @default.
- W2085279970 hasAuthorship W2085279970A5073866582 @default.
- W2085279970 hasAuthorship W2085279970A5084637239 @default.
- W2085279970 hasConcept C104317684 @default.
- W2085279970 hasConcept C116084860 @default.
- W2085279970 hasConcept C12554922 @default.
- W2085279970 hasConcept C127413603 @default.
- W2085279970 hasConcept C135983454 @default.
- W2085279970 hasConcept C14631669 @default.
- W2085279970 hasConcept C153356533 @default.
- W2085279970 hasConcept C155672457 @default.
- W2085279970 hasConcept C171250308 @default.
- W2085279970 hasConcept C178790620 @default.
- W2085279970 hasConcept C185592680 @default.
- W2085279970 hasConcept C192562407 @default.
- W2085279970 hasConcept C22744801 @default.
- W2085279970 hasConcept C25642318 @default.
- W2085279970 hasConcept C2778271344 @default.
- W2085279970 hasConcept C2779399885 @default.
- W2085279970 hasConcept C2779732960 @default.
- W2085279970 hasConcept C2780749602 @default.
- W2085279970 hasConcept C40250595 @default.
- W2085279970 hasConcept C42360764 @default.
- W2085279970 hasConcept C43617362 @default.
- W2085279970 hasConcept C521977710 @default.
- W2085279970 hasConcept C54009773 @default.
- W2085279970 hasConcept C552990157 @default.
- W2085279970 hasConcept C55493867 @default.
- W2085279970 hasConcept C86181022 @default.
- W2085279970 hasConcept C86803240 @default.
- W2085279970 hasConceptScore W2085279970C104317684 @default.
- W2085279970 hasConceptScore W2085279970C116084860 @default.
- W2085279970 hasConceptScore W2085279970C12554922 @default.
- W2085279970 hasConceptScore W2085279970C127413603 @default.
- W2085279970 hasConceptScore W2085279970C135983454 @default.
- W2085279970 hasConceptScore W2085279970C14631669 @default.
- W2085279970 hasConceptScore W2085279970C153356533 @default.
- W2085279970 hasConceptScore W2085279970C155672457 @default.
- W2085279970 hasConceptScore W2085279970C171250308 @default.
- W2085279970 hasConceptScore W2085279970C178790620 @default.
- W2085279970 hasConceptScore W2085279970C185592680 @default.
- W2085279970 hasConceptScore W2085279970C192562407 @default.
- W2085279970 hasConceptScore W2085279970C22744801 @default.
- W2085279970 hasConceptScore W2085279970C25642318 @default.
- W2085279970 hasConceptScore W2085279970C2778271344 @default.
- W2085279970 hasConceptScore W2085279970C2779399885 @default.