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- W2085283923 abstract "Targeted enrichment and duplex sequencing yield extremely efficient, accurate and sensitive sequencing of rare subclonal mutations in small genomic target regions. The detection of minority variants in mixed samples requires methods for enrichment and accurate sequencing of small genomic intervals. We describe an efficient approach based on sequential rounds of hybridization with biotinylated oligonucleotides that enables more than 1-million-fold enrichment of genomic regions of interest. In conjunction with error-correcting double-stranded molecular tags, our approach enables the quantification of mutations in individual DNA molecules." @default.
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- W2085283923 date "2015-04-06" @default.
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- W2085283923 title "Sequencing small genomic targets with high efficiency and extreme accuracy" @default.
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- W2085283923 doi "https://doi.org/10.1038/nmeth.3351" @default.
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