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- W2085335320 abstract "Mycoplasma mycoides strains were screened for the ability to produce H2O2 from glucose and glycerol metabolism using rapid and simple colorimetric assays. In quantitative assays, H2O2 production by washed cell suspensions was detected by the oxidation of o-dianisidine in the presence of peroxidase. In qualitative assays, a 3,3′-diaminobenzidine-peroxidase reagent was applied to colonies on agar plates. Both methods enabled differentiation of European subsp. mycoides SC (small colony) isolates from other M. mycoides strains by their inability to produce H2O2 from glycerol metabolism. In addition, two strains of subsp. capri were identified which produced large amounts of H2O2 from glucose oxidation. In lysed cells of these strains, NADH oxidation gave approximately 1 mol H2O2 per mol NADH oxidised whereas in 36 subsp. mycoides and 10 other subsp. capri strains, the quantity produced was 0.01–0.20 mol H2O2 per mol NADH oxidised." @default.
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- W2085335320 date "2001-02-01" @default.
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- W2085335320 title "Rapid screening of H2O2 production by Mycoplasma mycoides and differentiation of European subsp. mycoides SC (small colony) isolates" @default.
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- W2085335320 doi "https://doi.org/10.1016/s0378-1135(00)00305-9" @default.
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