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• W2085337301 abstract "Summary Endoplasmic reticulum (ER) stress has been shown to participate in many diseasepathologies. Although recent reports have demonstrated that ER stress in chondro-cytes is present in human osteoarthritis (OA), its role in the pathology of cartilagedegeneration, such as chondrocyte apoptosis, remains unclear. In the present study,we investigated the expression of phosphorylated PERK (pPERK), ubiquitin (Ub),GRP78, CHOP, phosphorylated JNK (pJNK) and cleaved caspase-3 (C-CASP3) andthe mRNA splicing of XBP1 (XBP1 splicing) in human OA cartilage by immunohis-tochemistry and RT-PCR. Additionally, human chondrocytes were treated with sev-eral concentrations of tunicamycin, an ER stress inducer, to assess the impact of ERstress on the mRNA expression of CHOP, XBP1 splicing and apoptosis, as deter-mined by real-time PCR, RT-PCR and ELISA analyses respectively. In human OAcartilage, the number of chondrocytes expressing pPERK, Ub, CHOP and pJNK pos-itively correlated with cartilage degeneration and the number of C-CASP3-positivechondrocytes. XBP1 splicing and GRP78 expression in severe OA containing thegreatest number of C-CASP3-positive chondrocytes were similar to the levels in mildOA, however, XBP1 splicing was higher in moderate OA than in mild and severeOA. Tunicamycin dose dependently increased CHOP expression and apoptosis ofcultured chondrocytes. Although tunicamycin upregulated XBP1 splicing in culturedchondrocytes, its impact on XBP1 splicing was weakened at higher concentrations.In conclusion, the present results indicate that ER stress may contribute to chondro-cyte apoptosis along with OA progression, which was closely associated with anenhanced apoptotic response and a reduced protective response by the cells.Keywordsapoptosis, cartilage, endoplasmic reticulum stress, osteoarthritis" @default.
• W2085337301 created "2016-06-24" @default.
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• W2085337301 date "2011-09-01" @default.
• W2085337301 modified "2023-09-27" @default.
• W2085337301 title "205 ENHANCED APOPTOTIC AND REDUCED PROTECTIVE RESPONSE IN CHONDROCYTES FOLLOWING ENDOPLASMIC RETICULUM STRESS IN OSTEOARTHRHIC CARTILAGE" @default.
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• W2085337301 doi "https://doi.org/10.1016/s1063-4584(11)60232-x" @default.
• W2085337301 hasPublicationYear "2011" @default.
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