Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085363313> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2085363313 endingPage "30" @default.
- W2085363313 startingPage "25" @default.
- W2085363313 abstract "In this study we examine whether different TK1 variants of pI 6.9 and 8.3 found by isoelectric focusing gel electrophoresis (IFE) reflect just a phenotype difference due to phosphorylation modifications or have a real phenotypic background. The phosphorylation degree of purified TK1 variants was analyzed by determining the changes in the pI values after treatment with alkaline phosphatase, using IFE. The genetic origin of the two TK1 variants was studied by determining their mol wt. by means of SDS-gelelectrophoresis. Furthermore, the subcellular distribution of the two TK1 variants was also studied. Alkaline phosphatase treatment changed the pI value of purified TK1 from 6.9 to 8.3. No change in the pI value was found when purified TK1 corresponding to pI 8.3 was treated in the same way. Similar results were obtained when treated a cytosolic fraction with alkaline phosphatase. Antibody raised against the C-terminal part of human TK1 only recognized the dephosphorylated TK1 variant corresponding to pI 8.3. There was no difference in the molecular weight between the two TK1 variants. Thus, we concluded that the TK1 variants corresponding to pI 6.9 and 8.3 are of the same genetic origin, but consist of phosphorylated and dephosphorylated forms." @default.
- W2085363313 created "2016-06-24" @default.
- W2085363313 creator A5066749130 @default.
- W2085363313 creator A5072714134 @default.
- W2085363313 creator A5080074268 @default.
- W2085363313 creator A5085764989 @default.
- W2085363313 creator A5087478688 @default.
- W2085363313 date "1996-02-01" @default.
- W2085363313 modified "2023-10-03" @default.
- W2085363313 title "Existence of phosphorylated and dephosphorylated forms of cytosolic thymidine kinase (TK1)" @default.
- W2085363313 cites W1480164066 @default.
- W2085363313 cites W1493290470 @default.
- W2085363313 cites W1522987871 @default.
- W2085363313 cites W1591455649 @default.
- W2085363313 cites W1846390947 @default.
- W2085363313 cites W1876225060 @default.
- W2085363313 cites W1953641388 @default.
- W2085363313 cites W1972744319 @default.
- W2085363313 cites W2009238248 @default.
- W2085363313 cites W2025475196 @default.
- W2085363313 cites W2058128222 @default.
- W2085363313 cites W2075228155 @default.
- W2085363313 cites W2082961003 @default.
- W2085363313 cites W2089043840 @default.
- W2085363313 doi "https://doi.org/10.1016/0304-4165(95)00127-1" @default.
- W2085363313 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8605228" @default.
- W2085363313 hasPublicationYear "1996" @default.
- W2085363313 type Work @default.
- W2085363313 sameAs 2085363313 @default.
- W2085363313 citedByCount "7" @default.
- W2085363313 crossrefType "journal-article" @default.
- W2085363313 hasAuthorship W2085363313A5066749130 @default.
- W2085363313 hasAuthorship W2085363313A5072714134 @default.
- W2085363313 hasAuthorship W2085363313A5080074268 @default.
- W2085363313 hasAuthorship W2085363313A5085764989 @default.
- W2085363313 hasAuthorship W2085363313A5087478688 @default.
- W2085363313 hasConcept C101660505 @default.
- W2085363313 hasConcept C104317684 @default.
- W2085363313 hasConcept C11960822 @default.
- W2085363313 hasConcept C119795356 @default.
- W2085363313 hasConcept C127716648 @default.
- W2085363313 hasConcept C153911025 @default.
- W2085363313 hasConcept C160160445 @default.
- W2085363313 hasConcept C178666793 @default.
- W2085363313 hasConcept C181199279 @default.
- W2085363313 hasConcept C184235292 @default.
- W2085363313 hasConcept C185592680 @default.
- W2085363313 hasConcept C50476208 @default.
- W2085363313 hasConcept C53009064 @default.
- W2085363313 hasConcept C55493867 @default.
- W2085363313 hasConcept C86803240 @default.
- W2085363313 hasConcept C98539663 @default.
- W2085363313 hasConceptScore W2085363313C101660505 @default.
- W2085363313 hasConceptScore W2085363313C104317684 @default.
- W2085363313 hasConceptScore W2085363313C11960822 @default.
- W2085363313 hasConceptScore W2085363313C119795356 @default.
- W2085363313 hasConceptScore W2085363313C127716648 @default.
- W2085363313 hasConceptScore W2085363313C153911025 @default.
- W2085363313 hasConceptScore W2085363313C160160445 @default.
- W2085363313 hasConceptScore W2085363313C178666793 @default.
- W2085363313 hasConceptScore W2085363313C181199279 @default.
- W2085363313 hasConceptScore W2085363313C184235292 @default.
- W2085363313 hasConceptScore W2085363313C185592680 @default.
- W2085363313 hasConceptScore W2085363313C50476208 @default.
- W2085363313 hasConceptScore W2085363313C53009064 @default.
- W2085363313 hasConceptScore W2085363313C55493867 @default.
- W2085363313 hasConceptScore W2085363313C86803240 @default.
- W2085363313 hasConceptScore W2085363313C98539663 @default.
- W2085363313 hasIssue "1" @default.
- W2085363313 hasLocation W20853633131 @default.
- W2085363313 hasLocation W20853633132 @default.
- W2085363313 hasOpenAccess W2085363313 @default.
- W2085363313 hasPrimaryLocation W20853633131 @default.
- W2085363313 hasRelatedWork W19566292 @default.
- W2085363313 hasRelatedWork W1974585837 @default.
- W2085363313 hasRelatedWork W1986393148 @default.
- W2085363313 hasRelatedWork W1992430433 @default.
- W2085363313 hasRelatedWork W2024782539 @default.
- W2085363313 hasRelatedWork W2058545848 @default.
- W2085363313 hasRelatedWork W2060038873 @default.
- W2085363313 hasRelatedWork W2073077483 @default.
- W2085363313 hasRelatedWork W2114819079 @default.
- W2085363313 hasRelatedWork W2344223403 @default.
- W2085363313 hasVolume "1289" @default.
- W2085363313 isParatext "false" @default.
- W2085363313 isRetracted "false" @default.
- W2085363313 magId "2085363313" @default.
- W2085363313 workType "article" @default.