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- W2085363413 abstract "RNAi (RNA interference, RNA silencing) is a powerful tool for functional genomics, but the construction of an RNAi vector(s) and the establishment of stable transformants are time-consuming and laborious. Here we report the transient RNAi of endogenous biosynthetic genes involved in isoquinoline alkaloid biosynthesis in Coptis japonica protoplasts. Double stranded (ds) RNA fragments of various lengths prepared from several different positions of the coding sequence of scoulerine 9-O-methyltransferase (SMT) were introduced into C. japonica protoplasts by polyethylene glycol-mediated transformation, and their effects were monitored by reverse transcription-polymerase chain reaction. Substantial silencing of SMT gene expression was obtained by the introduction of these SMT dsRNAs. A significant reduction in SMT protein levels was also observed. The potentials of this transient RNAi system to evaluate the functions of biosynthetic genes in Coptis alkaloid research are discussed." @default.
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- W2085363413 date "2005-01-01" @default.
- W2085363413 modified "2023-10-16" @default.
- W2085363413 title "Transient RNA Silencing of Scoulerine 9-<i>O</i>-Methyltransferase Expression by Double Stranded RNA in<i>Coptis japonica</i>Protoplasts" @default.
- W2085363413 cites W2063125230 @default.
- W2085363413 doi "https://doi.org/10.1271/bbb.69.63" @default.
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