FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085364060> ?p ?o ?g. }

• W2085364060 endingPage "2181" @default.
• W2085364060 startingPage "2169" @default.
• W2085364060 abstract "We examined the metabolism and intracellular transport of the D-erythro and L-threo stereoisomers of a fluorescent analogue of sphingomyelin, N-(N-[6-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino] caproyl])-sphingosylphosphorylcholine (C6-NBD-SM), in Chinese hamster ovary (CHO-K1) fibroblast monolayers. C6-NBD-SM was integrated into the plasma membrane bilayer by transfer of C6-NBD-SM monomers from liposomes to cells at 7 degrees C. The cells were washed, and within 10-15 min of being warmed to 37 degrees C, C6-NBD-SM was internalized from the plasma membrane to a perinuclear location that colocalized with the centriole and was distinct from the lysosomes and the Golgi apparatus. This perinuclear region was also labeled by internalized rhodamine-conjugated transferrin. C6-NBD-SM endocytosis was not inhibited when the microtubules were disrupted with nocodazole; rather, the fluorescent lipid was distributed in vesicles throughout the cell periphery instead of being internalized to the perinuclear region of the cell. The metabolism of C6-NBD-SM to other fluorescent sphingolipids at 37 degrees C and its effect on C6-NBD-SM transport was also examined. To study plasma membrane lipid recycling, C6-NBD-SM was first inserted into the plasma membrane of CHO-K1 cells and then allowed to be internalized by the cells at 37 degrees C. Any C6-NBD-SM remaining at the plasma membrane was then removed by incubation with nonfluorescent liposomes at 7 degrees C, leaving cells containing only internalized fluorescent lipid. The return of C6-NBD-SM to the plasma membrane from intracellular compartments upon further 37 degrees C incubation was then observed. The half-time for a complete round C6-NBD-SM recycling between the plasma membrane and intracellular compartments was approximately 40 min. Pretreatment of cells with either monensin or nocodazole did not inhibit C6-NBD-SM recycling." @default.
• W2085364060 created "2016-06-24" @default.
• W2085364060 creator A5059359203 @default.
• W2085364060 creator A5082867899 @default.
• W2085364060 date "1989-06-01" @default.
• W2085364060 modified "2023-10-12" @default.
• W2085364060 title "Lipid recycling between the plasma membrane and intracellular compartments: transport and metabolism of fluorescent sphingomyelin analogues in cultured fibroblasts." @default.
• W2085364060 cites W1249686701 @default.
• W2085364060 cites W1561840692 @default.
• W2085364060 cites W1578213988 @default.
• W2085364060 cites W1596856326 @default.
• W2085364060 cites W1611493774 @default.
• W2085364060 cites W1965651743 @default.
• W2085364060 cites W1966129095 @default.
• W2085364060 cites W1970249314 @default.
• W2085364060 cites W1974258163 @default.
• W2085364060 cites W1976652049 @default.
• W2085364060 cites W1977779461 @default.
• W2085364060 cites W1978707797 @default.
• W2085364060 cites W1985197668 @default.
• W2085364060 cites W1997007023 @default.
• W2085364060 cites W2006585409 @default.
• W2085364060 cites W2011912954 @default.
• W2085364060 cites W2015821566 @default.
• W2085364060 cites W2027899810 @default.
• W2085364060 cites W2029108634 @default.
• W2085364060 cites W2034863466 @default.
• W2085364060 cites W2059526131 @default.
• W2085364060 cites W2060334613 @default.
• W2085364060 cites W2062595910 @default.
• W2085364060 cites W2067202413 @default.
• W2085364060 cites W2067856724 @default.
• W2085364060 cites W2071980159 @default.
• W2085364060 cites W2079687293 @default.
• W2085364060 cites W2106508078 @default.
• W2085364060 cites W2110408216 @default.
• W2085364060 cites W2111930154 @default.
• W2085364060 cites W2130452852 @default.
• W2085364060 cites W2138466097 @default.
• W2085364060 cites W2142539904 @default.
• W2085364060 cites W2143105912 @default.
• W2085364060 cites W2144892927 @default.
• W2085364060 cites W2152311269 @default.
• W2085364060 cites W2159153404 @default.
• W2085364060 cites W2163264786 @default.
• W2085364060 cites W2175885977 @default.
• W2085364060 doi "https://doi.org/10.1083/jcb.108.6.2169" @default.
• W2085364060 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2115574" @default.
• W2085364060 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2738091" @default.
• W2085364060 hasPublicationYear "1989" @default.
• W2085364060 type Work @default.
• W2085364060 sameAs 2085364060 @default.
• W2085364060 citedByCount "210" @default.
• W2085364060 countsByYear W20853640602012 @default.
• W2085364060 countsByYear W20853640602013 @default.
• W2085364060 countsByYear W20853640602014 @default.
• W2085364060 countsByYear W20853640602015 @default.
• W2085364060 countsByYear W20853640602016 @default.
• W2085364060 countsByYear W20853640602017 @default.
• W2085364060 countsByYear W20853640602018 @default.
• W2085364060 countsByYear W20853640602019 @default.
• W2085364060 countsByYear W20853640602021 @default.
• W2085364060 countsByYear W20853640602022 @default.
• W2085364060 countsByYear W20853640602023 @default.
• W2085364060 crossrefType "journal-article" @default.
• W2085364060 hasAuthorship W2085364060A5059359203 @default.
• W2085364060 hasAuthorship W2085364060A5082867899 @default.
• W2085364060 hasBestOaLocation W20853640601 @default.
• W2085364060 hasConcept C102747710 @default.
• W2085364060 hasConcept C119062480 @default.
• W2085364060 hasConcept C12554922 @default.
• W2085364060 hasConcept C130316041 @default.
• W2085364060 hasConcept C142669718 @default.
• W2085364060 hasConcept C1491633281 @default.
• W2085364060 hasConcept C158617107 @default.
• W2085364060 hasConcept C170493617 @default.
• W2085364060 hasConcept C175656101 @default.
• W2085364060 hasConcept C185154212 @default.
• W2085364060 hasConcept C2779363559 @default.
• W2085364060 hasConcept C28005876 @default.
• W2085364060 hasConcept C41625074 @default.
• W2085364060 hasConcept C55493867 @default.
• W2085364060 hasConcept C61322309 @default.
• W2085364060 hasConcept C79879829 @default.
• W2085364060 hasConcept C86803240 @default.
• W2085364060 hasConcept C95444343 @default.
• W2085364060 hasConceptScore W2085364060C102747710 @default.
• W2085364060 hasConceptScore W2085364060C119062480 @default.
• W2085364060 hasConceptScore W2085364060C12554922 @default.
• W2085364060 hasConceptScore W2085364060C130316041 @default.
• W2085364060 hasConceptScore W2085364060C142669718 @default.
• W2085364060 hasConceptScore W2085364060C1491633281 @default.
• W2085364060 hasConceptScore W2085364060C158617107 @default.
• W2085364060 hasConceptScore W2085364060C170493617 @default.
• W2085364060 hasConceptScore W2085364060C175656101 @default.
• W2085364060 hasConceptScore W2085364060C185154212 @default.
• W2085364060 hasConceptScore W2085364060C2779363559 @default.
• W2085364060 hasConceptScore W2085364060C28005876 @default.

• next