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• W2085371331 startingPage "2902" @default.
• W2085371331 abstract "Selections mediate transient adhesion of neutrophils to stimulated endothelial cells at sites of inflammation by binding counter-receptors that present carbohydrates such as sialyl Lewis(x). We have developed a cell-free adhesion assay using sialyl Lewis(x)-coated microspheres and E-selection-IgG chimera-coated substrates to investigate the premise that rolling primarily results from functional properties of selection-carbohydrate bonds, whereas cellular morphology and signaling act as secondary effects. Sialyl Lewis(x)-coated microspheres attach to and roll over E-selectin-IgG chimera-coated substrates between the physiological wall shear stresses of 0.7 and 2 dynes/cm2. Rolling velocities vary with time and depend on E-selectin-IgG chimera site density and wall shear stress. Our results show that sialyl Lewis(x) is a minimal functional recognition element required for rolling on E-selectin under flow." @default.
• W2085371331 created "2016-06-24" @default.
• W2085371331 creator A5012194910 @default.
• W2085371331 creator A5051971809 @default.
• W2085371331 creator A5059067804 @default.
• W2085371331 date "1996-11-01" @default.
• W2085371331 modified "2023-09-27" @default.
• W2085371331 title "Sialyl Lewis(x)/E-selectin-mediated rolling in a cell-free system" @default.
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• W2085371331 doi "https://doi.org/10.1016/s0006-3495(96)79487-0" @default.
• W2085371331 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1233776" @default.
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