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• W2085380046 abstract "PURPOSE. To investigate the antiproliferative effect of ionizing radiation on retinal pigment epithelial (RPE) cells that are sup-posed to play a major role in the pathogenesis of proliferative vitreoretinopathy (PVR). METHODS. RPE cells from pig eyes were irradiated with doses ranging from 4 to 16 Gy (1 Gray = 1 Joule/kilogram). Cells were counted at 1, 2, 3, and 4 weeks (Experiment 1) or 1, 2, 4, and 6 weeks (Experiment 2) after treatment. In Experiment 3, cells were trypsinized 24 h after radiation and seeded again. Colonies were counted 10 days later, and the surviving fraction was determined. RESULTS. The numbers of cells and colonies were inversely cor-related to the doses applied. In Experiment 2, cell numbers of radiated cultures remained stable during the time of follow-up, whereas, in Experiment 1, significant proliferation occurred in treated cultures as well as in controls. This may be due to the higher growing rate that was found in the cultures of Experi-ment 2, compared to those of Experiment 1, at the time of radi-ation. In Experiment 3, a D0 value of 0.72 Gy was found. CONCLUSIOS. Proliferation of RPE cells can be suppressed by irradiation in a dose-dependent manner. Therefore, radiother-apy may be useful in the treatment of PVR. Its effect probably depends on the stage or activity of PVR at the time of radia-tion." @default.
• W2085380046 created "2016-06-24" @default.
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• W2085380046 date "1997-01-01" @default.
• W2085380046 modified "2023-09-26" @default.
• W2085380046 title "Radiation response of porcine RPE cells in vitro" @default.
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• W2085380046 doi "https://doi.org/10.1076/ceyr.16.11.1142.5110" @default.
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