FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085382507> ?p ?o ?g. }

• W2085382507 endingPage "739" @default.
• W2085382507 startingPage "727" @default.
• W2085382507 abstract "We report for the first time the detection of membrane lipid rafts in mouse oocytes and cleaving preimplantation embryos. Cholera toxin beta (CTbeta), which binds to the raft-enriched ganglioside GM1, was selected to label rafts. In a novel application a Qdot reagent was used to detect CTbeta labeling. This is the first reported use of nanocrystals in mammalian embryo imaging. Comparative membrane labeling with CTbeta and lipophilic membrane dyes containing saturated or unsaturated aliphatic tails showed that the detection of GM1 in mouse oocytes and embryo membranes was consistent with the identification of cholesterol- and sphingolipid-enriched rafts in the cell membrane. Distribution of the GM1 was compared with the known distribution of non-raft membrane components, and disruption of membrane rafts with detergents confirmed the cholesterol dependence of GM1 on lipid raft labeling. Complementary functional studies showed that cholesterol depletion using methyl-beta-cyclodextrin inhibited preimplantation development in culture. Our results show that the membranes of the mouse oocyte and zygote are rich in lipid rafts, with heterogeneous and stage-dependent distribution. In dividing embryos, the rafts were clearly associated with the cleavage furrow. At the morula stage, rafts were also apically enriched in each blastomere. In blastocysts, rafts were detectable in the trophectoderm layer, but could not be detected in the inner cell mass without prior fixation and permeabilization of the embryo. Lipid rafts and their associated proteins are, therefore, spatio-temporally positioned to a play a critical role in preimplantation developmental events." @default.
• W2085382507 created "2016-06-24" @default.
• W2085382507 creator A5025524173 @default.
• W2085382507 creator A5035312083 @default.
• W2085382507 date "2007-03-01" @default.
• W2085382507 modified "2023-09-27" @default.
• W2085382507 title "Spatio-temporal localization of membrane lipid rafts in mouse oocytes and cleaving preimplantation embryos" @default.
• W2085382507 cites W1564861448 @default.
• W2085382507 cites W1589597980 @default.
• W2085382507 cites W1784927340 @default.
• W2085382507 cites W1885525264 @default.
• W2085382507 cites W1968480824 @default.
• W2085382507 cites W1970868451 @default.
• W2085382507 cites W1974642741 @default.
• W2085382507 cites W1975235201 @default.
• W2085382507 cites W1990565440 @default.
• W2085382507 cites W2000454974 @default.
• W2085382507 cites W2001478996 @default.
• W2085382507 cites W2010936482 @default.
• W2085382507 cites W2014030143 @default.
• W2085382507 cites W2014903703 @default.
• W2085382507 cites W2018315382 @default.
• W2085382507 cites W2018680653 @default.
• W2085382507 cites W2019455762 @default.
• W2085382507 cites W2059694983 @default.
• W2085382507 cites W2066485484 @default.
• W2085382507 cites W2074736428 @default.
• W2085382507 cites W2080386975 @default.
• W2085382507 cites W2085261477 @default.
• W2085382507 cites W2086061852 @default.
• W2085382507 cites W2097470791 @default.
• W2085382507 cites W2098179426 @default.
• W2085382507 cites W2105979046 @default.
• W2085382507 cites W2108598406 @default.
• W2085382507 cites W2120062443 @default.
• W2085382507 cites W2131252883 @default.
• W2085382507 cites W2135164336 @default.
• W2085382507 cites W2141367941 @default.
• W2085382507 cites W2148924929 @default.
• W2085382507 cites W2153419157 @default.
• W2085382507 cites W2153756342 @default.
• W2085382507 cites W2170963117 @default.
• W2085382507 cites W2343043741 @default.
• W2085382507 cites W2421746682 @default.
• W2085382507 cites W4240075844 @default.
• W2085382507 cites W42618 @default.
• W2085382507 doi "https://doi.org/10.1016/j.ydbio.2006.12.009" @default.
• W2085382507 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1861839" @default.
• W2085382507 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17258703" @default.
• W2085382507 hasPublicationYear "2007" @default.
• W2085382507 type Work @default.
• W2085382507 sameAs 2085382507 @default.
• W2085382507 citedByCount "41" @default.
• W2085382507 countsByYear W20853825072012 @default.
• W2085382507 countsByYear W20853825072013 @default.
• W2085382507 countsByYear W20853825072015 @default.
• W2085382507 countsByYear W20853825072016 @default.
• W2085382507 countsByYear W20853825072017 @default.
• W2085382507 countsByYear W20853825072018 @default.
• W2085382507 countsByYear W20853825072019 @default.
• W2085382507 countsByYear W20853825072021 @default.
• W2085382507 countsByYear W20853825072022 @default.
• W2085382507 countsByYear W20853825072023 @default.
• W2085382507 crossrefType "journal-article" @default.
• W2085382507 hasAuthorship W2085382507A5025524173 @default.
• W2085382507 hasAuthorship W2085382507A5035312083 @default.
• W2085382507 hasBestOaLocation W20853825072 @default.
• W2085382507 hasConcept C1491633281 @default.
• W2085382507 hasConcept C15920480 @default.
• W2085382507 hasConcept C178790620 @default.
• W2085382507 hasConcept C185592680 @default.
• W2085382507 hasConcept C196843134 @default.
• W2085382507 hasConcept C2776690073 @default.
• W2085382507 hasConcept C2778106830 @default.
• W2085382507 hasConcept C28005876 @default.
• W2085382507 hasConcept C28748434 @default.
• W2085382507 hasConcept C41625074 @default.
• W2085382507 hasConcept C521977710 @default.
• W2085382507 hasConcept C55493867 @default.
• W2085382507 hasConcept C61705674 @default.
• W2085382507 hasConcept C62478195 @default.
• W2085382507 hasConcept C68760520 @default.
• W2085382507 hasConcept C79266657 @default.
• W2085382507 hasConcept C86803240 @default.
• W2085382507 hasConcept C87073359 @default.
• W2085382507 hasConcept C9132879 @default.
• W2085382507 hasConcept C95444343 @default.
• W2085382507 hasConceptScore W2085382507C1491633281 @default.
• W2085382507 hasConceptScore W2085382507C15920480 @default.
• W2085382507 hasConceptScore W2085382507C178790620 @default.
• W2085382507 hasConceptScore W2085382507C185592680 @default.
• W2085382507 hasConceptScore W2085382507C196843134 @default.
• W2085382507 hasConceptScore W2085382507C2776690073 @default.
• W2085382507 hasConceptScore W2085382507C2778106830 @default.
• W2085382507 hasConceptScore W2085382507C28005876 @default.
• W2085382507 hasConceptScore W2085382507C28748434 @default.
• W2085382507 hasConceptScore W2085382507C41625074 @default.
• W2085382507 hasConceptScore W2085382507C521977710 @default.

• next