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- W2085400513 abstract "-Three agglutinins have been isolated from the aqueous ethanolic extract of the marine red alga Solieria robusta by precipitation with cold ethanol, gel filtration and ion-exchange HPLC. These new proteins, designated solnins A, B and C, were monomeric glycoproteins with a similar Mr of 23 000 from gel filtration and 29 000 from SDS-electrophoresis, and they shared such predominant amino acids as Gly, Asx and Glx. However, solnins A, B and C had different isoelectric points of 4.3, 4.2 and 4.1, respectively. From electrophoretic analyses of these agglutinins on polyacrylamide gels of various concentration, they were confirmed to be isoagglutinins. The ratio of the yields of solnins A, B and C was ca 1:3:1. Solnins strongly agglutinated rabbit erythrocytes, but did not agglutinate human erythrocytes and mouse FM3A tumour cells. Treatment of the rabbit erythrocytes with trypsin or pronase effected their sensitivity to hemagglutination by the agglutinins. The hemagglutinating activity of the solnins was inhibited by glycoproteins bearing N- glycosidic sugar chains, but not by any mono- and oligosaccharides tested. The activity was not affected by divalent cations. Solnins also showed mitogenic activity for mouse splenic lymphocytes, while they inhibited the growth in vitro of mouse leukaemia cells L1210 or mouse FM3A tumour cells." @default.
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- W2085400513 date "1988-01-01" @default.
- W2085400513 modified "2023-09-26" @default.
- W2085400513 title "Mitogenic and antineoplastic isoagglutinins from the red alga Solieria robusta" @default.
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- W2085400513 doi "https://doi.org/10.1016/0031-9422(88)80097-9" @default.
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