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- W2085405513 abstract "This chapter describes the procedures for obtaining highly purified eukaryotic initiation factors (eIF) eIF-2, eIF-3, eIF-4A, eIF-4B, eIF-4F, partially purified eIF-5, and highly purified elongation factors (EF) EF-Iα, EF-Iβγ, and EF-2 from wheat germ. Initiation of the translation of messenger RNA (mRNA) on cytoplasmic ribosomes of eukaryotic cells is a complicated process requiring Met-transfer RNA (tRNA), Guanosine-5'-triphosphate (GTP), Adenosine-5'-triphosphate (ATP), 10 initiation factors, and one or more ancillary factors. The chapter also describes some of the physical and functional properties of these factors. It also outlines the assay for elongation factors, EF-Iα, EF-Iβγ, and EF-2 and the assay for initiation factors, eIF-3, eIF-4A, eIF-4B, eIF-4F, and eIF-5. The elongation factors are measured by their ability to support poly (U)-directed polyphenylalanine synthesis." @default.
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- W2085405513 date "2007-01-01" @default.
- W2085405513 modified "2023-09-26" @default.
- W2085405513 title "Characterization of SCS as a potent activator for the CK1-mediated phosphorylation of tau protein and its associated proteins from rat brain" @default.
- W2085405513 doi "https://doi.org/10.1016/j.neures.2007.06.875" @default.
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