FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085451384> ?p ?o ?g. }

• W2085451384 endingPage "412" @default.
• W2085451384 startingPage "402" @default.
• W2085451384 abstract "This study was undertaken to generate rat offspring via tetraploid blastocyst complementation with embryonic stem (ES) cells. Tetraploid blastocysts were prepared by electrofusion of blastomeres from two-cell stage embryos, and subsequent in vivo culture for 4 days. Microinjection into the tetraploid blastocoel of an inner cell mass isolated by immunosurgery resulted in the generation of rat offspring, suggesting the successful contribution of tetraploid blastocysts to their placenta. Tetraploid blastocyst complementation was attempted with a total of 4 ES cell lines (2 lines of female karyotype and 2 lines of male karyotype). In the rESWIv-3i-5 (XX) cell line, normal-sized fetuses with heartbeats were harvested on E11.5 (12.1%), E12.5 (9.5%), and E13.5 (9.1%), but no viable fetuses were detected on E14.5. Similarly, use of the rESWIv-3i-1 (XX) cell line resulted in no viable fetus production on E14.5. Using the rESBLK2i-1 (XY) cell line, viable fetuses were harvested not only on E11.5–E13.5 (2.6–5.5%), but also on E14.5 (3.0%). The transfer of a total of 487 tetraploid blastocysts complemented with rESBLK2i-1 cells resulted in 256 implantation sites (52.6%) on E21.5, but no viable offspring was detected. Use of the rESBLK2i-1/huKO (XY) cell line also resulted in no viable offspring production on E21.5. Analyses of the methylation pattern in differentially methylated regions and transcript level of genes that are imprinted in mice (H19, Meg3, Igf2r, Peg5, and Peg10) in the E14.5 conceptuses indicated a marked difference between the ES cell-derived and control normal fetuses, but not between the tetraploid and control diploid placenta. Mol. Reprod. Dev. 79:402–412, 2012. © 2012 Wiley Periodicals, Inc." @default.
• W2085451384 created "2016-06-24" @default.
• W2085451384 creator A5012180883 @default.
• W2085451384 creator A5014869778 @default.
• W2085451384 creator A5027939725 @default.
• W2085451384 creator A5059937255 @default.
• W2085451384 creator A5076897990 @default.
• W2085451384 creator A5077244073 @default.
• W2085451384 creator A5080154032 @default.
• W2085451384 creator A5090468562 @default.
• W2085451384 date "2012-05-04" @default.
• W2085451384 modified "2023-10-14" @default.
• W2085451384 title "Ability of tetraploid rat blastocysts to support fetal development after complementation with embryonic stem cells" @default.
• W2085451384 cites W1652680663 @default.
• W2085451384 cites W1856781683 @default.
• W2085451384 cites W1911709771 @default.
• W2085451384 cites W1961597426 @default.
• W2085451384 cites W1966697925 @default.
• W2085451384 cites W1968581446 @default.
• W2085451384 cites W1972298775 @default.
• W2085451384 cites W1973107120 @default.
• W2085451384 cites W1988805575 @default.
• W2085451384 cites W1993353173 @default.
• W2085451384 cites W2000079859 @default.
• W2085451384 cites W2006844115 @default.
• W2085451384 cites W2008530548 @default.
• W2085451384 cites W2013269463 @default.
• W2085451384 cites W2014365914 @default.
• W2085451384 cites W2018658588 @default.
• W2085451384 cites W2034219651 @default.
• W2085451384 cites W2043952117 @default.
• W2085451384 cites W2044380781 @default.
• W2085451384 cites W2045899482 @default.
• W2085451384 cites W2051555664 @default.
• W2085451384 cites W2054857432 @default.
• W2085451384 cites W2057159461 @default.
• W2085451384 cites W2058521801 @default.
• W2085451384 cites W2066152940 @default.
• W2085451384 cites W2067417503 @default.
• W2085451384 cites W2075857997 @default.
• W2085451384 cites W2075964621 @default.
• W2085451384 cites W2081243436 @default.
• W2085451384 cites W2082082936 @default.
• W2085451384 cites W2096592632 @default.
• W2085451384 cites W2109744103 @default.
• W2085451384 cites W2110623684 @default.
• W2085451384 cites W2118445352 @default.
• W2085451384 cites W2119419499 @default.
• W2085451384 cites W2121468306 @default.
• W2085451384 cites W2153130017 @default.
• W2085451384 cites W2170222722 @default.
• W2085451384 doi "https://doi.org/10.1002/mrd.22043" @default.
• W2085451384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22499253" @default.
• W2085451384 hasPublicationYear "2012" @default.
• W2085451384 type Work @default.
• W2085451384 sameAs 2085451384 @default.
• W2085451384 citedByCount "12" @default.
• W2085451384 countsByYear W20854513842012 @default.
• W2085451384 countsByYear W20854513842014 @default.
• W2085451384 countsByYear W20854513842015 @default.
• W2085451384 countsByYear W20854513842017 @default.
• W2085451384 countsByYear W20854513842018 @default.
• W2085451384 countsByYear W20854513842020 @default.
• W2085451384 countsByYear W20854513842021 @default.
• W2085451384 countsByYear W20854513842022 @default.
• W2085451384 crossrefType "journal-article" @default.
• W2085451384 hasAuthorship W2085451384A5012180883 @default.
• W2085451384 hasAuthorship W2085451384A5014869778 @default.
• W2085451384 hasAuthorship W2085451384A5027939725 @default.
• W2085451384 hasAuthorship W2085451384A5059937255 @default.
• W2085451384 hasAuthorship W2085451384A5076897990 @default.
• W2085451384 hasAuthorship W2085451384A5077244073 @default.
• W2085451384 hasAuthorship W2085451384A5080154032 @default.
• W2085451384 hasAuthorship W2085451384A5090468562 @default.
• W2085451384 hasConcept C104317684 @default.
• W2085451384 hasConcept C112672928 @default.
• W2085451384 hasConcept C145103041 @default.
• W2085451384 hasConcept C153911025 @default.
• W2085451384 hasConcept C159399396 @default.
• W2085451384 hasConcept C16685009 @default.
• W2085451384 hasConcept C172680121 @default.
• W2085451384 hasConcept C196843134 @default.
• W2085451384 hasConcept C202438428 @default.
• W2085451384 hasConcept C2777804106 @default.
• W2085451384 hasConcept C2778177303 @default.
• W2085451384 hasConcept C2779234561 @default.
• W2085451384 hasConcept C30481170 @default.
• W2085451384 hasConcept C53226629 @default.
• W2085451384 hasConcept C54355233 @default.
• W2085451384 hasConcept C62525196 @default.
• W2085451384 hasConcept C68760520 @default.
• W2085451384 hasConcept C71924100 @default.
• W2085451384 hasConcept C86803240 @default.
• W2085451384 hasConcept C87073359 @default.
• W2085451384 hasConcept C95444343 @default.
• W2085451384 hasConceptScore W2085451384C104317684 @default.
• W2085451384 hasConceptScore W2085451384C112672928 @default.
• W2085451384 hasConceptScore W2085451384C145103041 @default.

• next