Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085452545> ?p ?o ?g. }
- W2085452545 endingPage "28" @default.
- W2085452545 startingPage "21" @default.
- W2085452545 abstract "Vascular endothelial growth factor (VEGF) up-regulates a number of cellular survival signals in endothelial cells. We hypothesize that VEGF will up-regulate survivin, a member of the IAP family of anti-apoptotic proteins, via the PI3K/Akt cell signaling pathway in human neuroblastoma cells.IMR-32 human neuroblastoma cells are cultured with VEGF at varying times and in escalating doses. A specific inhibitor of PI3-kinase, LY294002, is used to block Akt phosphorylation. Immunoblot is used to measure protein expression, and Hoechst staining is used to detect apoptosis.Stimulation of IMR-32 neuroblastoma cells with VEGF results in an increase in survivin protein expression in both a dose- and a time-dependent fashion. Akt phosphorylation is also increased after stimulation with exogenous VEGF. Blockade of Akt phosphorylation with LY294002 abrogates the effects of VEGF upon survivin and phosphorylated Akt protein expression.VEGF has been shown to up-regulate a number of survival signals in endothelial cells. We have found that exposure of human neuroblastoma cells to exogenous VEGF results in an increased expression of survivin protein and phosphorylated Akt, and inhibition of PI3-kinase abrogates those effects. It appears that VEGF is important for promotion of neuroblastoma cellular survival through the up-regulation of survival proteins, and not only through its angiogenic properties." @default.
- W2085452545 created "2016-06-24" @default.
- W2085452545 creator A5060333976 @default.
- W2085452545 creator A5071449143 @default.
- W2085452545 creator A5075780491 @default.
- W2085452545 creator A5080088331 @default.
- W2085452545 creator A5081171198 @default.
- W2085452545 date "2005-07-01" @default.
- W2085452545 modified "2023-10-16" @default.
- W2085452545 title "VEGF-Mediated Survivin Expression in Neuroblastoma Cells" @default.
- W2085452545 cites W1529396499 @default.
- W2085452545 cites W1589415602 @default.
- W2085452545 cites W1595083214 @default.
- W2085452545 cites W1684533593 @default.
- W2085452545 cites W1794577973 @default.
- W2085452545 cites W1967065056 @default.
- W2085452545 cites W1979494660 @default.
- W2085452545 cites W1980969801 @default.
- W2085452545 cites W1985804036 @default.
- W2085452545 cites W1991806275 @default.
- W2085452545 cites W1998713471 @default.
- W2085452545 cites W2004952608 @default.
- W2085452545 cites W2007466152 @default.
- W2085452545 cites W2008700666 @default.
- W2085452545 cites W2011417221 @default.
- W2085452545 cites W2014785934 @default.
- W2085452545 cites W2018958767 @default.
- W2085452545 cites W2022000183 @default.
- W2085452545 cites W2028466368 @default.
- W2085452545 cites W2031511203 @default.
- W2085452545 cites W2031970502 @default.
- W2085452545 cites W2035523654 @default.
- W2085452545 cites W2037761545 @default.
- W2085452545 cites W2039791984 @default.
- W2085452545 cites W2049345988 @default.
- W2085452545 cites W2051440419 @default.
- W2085452545 cites W2052010120 @default.
- W2085452545 cites W2060942394 @default.
- W2085452545 cites W2062627489 @default.
- W2085452545 cites W2070088887 @default.
- W2085452545 cites W2071525349 @default.
- W2085452545 cites W2073125132 @default.
- W2085452545 cites W2075977076 @default.
- W2085452545 cites W2077222940 @default.
- W2085452545 cites W2083540484 @default.
- W2085452545 cites W2087523741 @default.
- W2085452545 cites W2092386950 @default.
- W2085452545 cites W2094883350 @default.
- W2085452545 cites W2097767970 @default.
- W2085452545 cites W2107444883 @default.
- W2085452545 cites W2115159354 @default.
- W2085452545 cites W2167490100 @default.
- W2085452545 cites W2170839629 @default.
- W2085452545 cites W4231769143 @default.
- W2085452545 cites W4247664461 @default.
- W2085452545 doi "https://doi.org/10.1016/j.jss.2005.03.009" @default.
- W2085452545 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15882878" @default.
- W2085452545 hasPublicationYear "2005" @default.
- W2085452545 type Work @default.
- W2085452545 sameAs 2085452545 @default.
- W2085452545 citedByCount "44" @default.
- W2085452545 countsByYear W20854525452012 @default.
- W2085452545 countsByYear W20854525452013 @default.
- W2085452545 countsByYear W20854525452014 @default.
- W2085452545 countsByYear W20854525452016 @default.
- W2085452545 countsByYear W20854525452018 @default.
- W2085452545 countsByYear W20854525452019 @default.
- W2085452545 countsByYear W20854525452021 @default.
- W2085452545 countsByYear W20854525452023 @default.
- W2085452545 crossrefType "journal-article" @default.
- W2085452545 hasAuthorship W2085452545A5060333976 @default.
- W2085452545 hasAuthorship W2085452545A5071449143 @default.
- W2085452545 hasAuthorship W2085452545A5075780491 @default.
- W2085452545 hasAuthorship W2085452545A5080088331 @default.
- W2085452545 hasAuthorship W2085452545A5081171198 @default.
- W2085452545 hasConcept C11960822 @default.
- W2085452545 hasConcept C146285616 @default.
- W2085452545 hasConcept C167734588 @default.
- W2085452545 hasConcept C185592680 @default.
- W2085452545 hasConcept C190283241 @default.
- W2085452545 hasConcept C2775975398 @default.
- W2085452545 hasConcept C2776715637 @default.
- W2085452545 hasConcept C2776912716 @default.
- W2085452545 hasConcept C2777025900 @default.
- W2085452545 hasConcept C502942594 @default.
- W2085452545 hasConcept C54355233 @default.
- W2085452545 hasConcept C55493867 @default.
- W2085452545 hasConcept C62478195 @default.
- W2085452545 hasConcept C75217442 @default.
- W2085452545 hasConcept C81885089 @default.
- W2085452545 hasConcept C86554907 @default.
- W2085452545 hasConcept C86803240 @default.
- W2085452545 hasConcept C95444343 @default.
- W2085452545 hasConceptScore W2085452545C11960822 @default.
- W2085452545 hasConceptScore W2085452545C146285616 @default.
- W2085452545 hasConceptScore W2085452545C167734588 @default.
- W2085452545 hasConceptScore W2085452545C185592680 @default.
- W2085452545 hasConceptScore W2085452545C190283241 @default.