FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085456885> ?p ?o ?g. }

• W2085456885 endingPage "3065" @default.
• W2085456885 startingPage "3057" @default.
• W2085456885 abstract "Viroids of the family Avsunviroidae , such as eggplant latent viroid (ELVd), contain hammerhead ribozymes and replicate in the chloroplasts of the host plant through an RNA-based symmetrical rolling-circle mechanism in which oligomeric RNAs of both polarity are processed to monomeric linear RNAs (by cleavage) and to monomeric circular RNAs (by ligation). Using an experimental system consisting of transplastomic lines of the alga Chlamydomonas reinhardtii , a mutational analysis of sequence and structural elements in the ELVd molecule that are involved in transcript processing in vivo in a chloroplastic context was carried out. A collection of six insertion and three deletion ELVd mutants was created and expressed in C. reinhardtii chloroplast. All mutants cleaved efficiently except for the control with an insertion inside the hammerhead ribozyme domain, supporting the prediction that this domain is necessary and sufficient to mediate transcript cleavage in vivo . However, two deletion mutants that cleaved efficiently showed ligation defects, indicating that during RNA circularization, other parts of the molecule are involved in addition to the hammerhead ribozyme domain. This is probably a quasi double-stranded structure present in the central part of the molecule which contains the ligation site in an internal loop. However, the mutations prevented the viroid from infecting its natural host, eggplant, indicating that they affected other essential functions in ELVd infectious cycle. The insertion in the terminal loop of the right upper hairpin of ELVd did not have this effect; it was tolerated and partially maintained in the progeny." @default.
• W2085456885 created "2016-06-24" @default.
• W2085456885 creator A5044334610 @default.
• W2085456885 creator A5073133979 @default.
• W2085456885 creator A5075393643 @default.
• W2085456885 creator A5084885859 @default.
• W2085456885 date "2009-12-01" @default.
• W2085456885 modified "2023-10-18" @default.
• W2085456885 title "Mutational analysis of eggplant latent viroid RNA processing in Chlamydomonas reinhardtii chloroplast" @default.
• W2085456885 cites W1512306945 @default.
• W2085456885 cites W1965230193 @default.
• W2085456885 cites W1966387415 @default.
• W2085456885 cites W1979695888 @default.
• W2085456885 cites W1986078051 @default.
• W2085456885 cites W1988989917 @default.
• W2085456885 cites W1990594409 @default.
• W2085456885 cites W1996334722 @default.
• W2085456885 cites W1997105428 @default.
• W2085456885 cites W1997619878 @default.
• W2085456885 cites W2001130066 @default.
• W2085456885 cites W2018575993 @default.
• W2085456885 cites W2022636524 @default.
• W2085456885 cites W2036177624 @default.
• W2085456885 cites W2042425685 @default.
• W2085456885 cites W2052062452 @default.
• W2085456885 cites W2052336952 @default.
• W2085456885 cites W2053028162 @default.
• W2085456885 cites W2055010450 @default.
• W2085456885 cites W2059747554 @default.
• W2085456885 cites W2063015324 @default.
• W2085456885 cites W2084943154 @default.
• W2085456885 cites W2093286216 @default.
• W2085456885 cites W2095300027 @default.
• W2085456885 cites W2096249174 @default.
• W2085456885 cites W2108670183 @default.
• W2085456885 cites W2114463902 @default.
• W2085456885 cites W2124037659 @default.
• W2085456885 cites W2135122666 @default.
• W2085456885 cites W2141157874 @default.
• W2085456885 cites W2144120226 @default.
• W2085456885 cites W2147129472 @default.
• W2085456885 cites W2162119552 @default.
• W2085456885 cites W2170328283 @default.
• W2085456885 cites W2170600923 @default.
• W2085456885 cites W50420956 @default.
• W2085456885 doi "https://doi.org/10.1099/vir.0.013425-0" @default.
• W2085456885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19675190" @default.
• W2085456885 hasPublicationYear "2009" @default.
• W2085456885 type Work @default.
• W2085456885 sameAs 2085456885 @default.
• W2085456885 citedByCount "20" @default.
• W2085456885 countsByYear W20854568852012 @default.
• W2085456885 countsByYear W20854568852015 @default.
• W2085456885 countsByYear W20854568852016 @default.
• W2085456885 countsByYear W20854568852017 @default.
• W2085456885 countsByYear W20854568852018 @default.
• W2085456885 countsByYear W20854568852022 @default.
• W2085456885 countsByYear W20854568852023 @default.
• W2085456885 crossrefType "journal-article" @default.
• W2085456885 hasAuthorship W2085456885A5044334610 @default.
• W2085456885 hasAuthorship W2085456885A5073133979 @default.
• W2085456885 hasAuthorship W2085456885A5075393643 @default.
• W2085456885 hasAuthorship W2085456885A5084885859 @default.
• W2085456885 hasBestOaLocation W20854568851 @default.
• W2085456885 hasConcept C104317684 @default.
• W2085456885 hasConcept C143065580 @default.
• W2085456885 hasConcept C153911025 @default.
• W2085456885 hasConcept C2777727519 @default.
• W2085456885 hasConcept C2779792011 @default.
• W2085456885 hasConcept C2780781446 @default.
• W2085456885 hasConcept C30951146 @default.
• W2085456885 hasConcept C54355233 @default.
• W2085456885 hasConcept C67705224 @default.
• W2085456885 hasConcept C76346623 @default.
• W2085456885 hasConcept C86803240 @default.
• W2085456885 hasConceptScore W2085456885C104317684 @default.
• W2085456885 hasConceptScore W2085456885C143065580 @default.
• W2085456885 hasConceptScore W2085456885C153911025 @default.
• W2085456885 hasConceptScore W2085456885C2777727519 @default.
• W2085456885 hasConceptScore W2085456885C2779792011 @default.
• W2085456885 hasConceptScore W2085456885C2780781446 @default.
• W2085456885 hasConceptScore W2085456885C30951146 @default.
• W2085456885 hasConceptScore W2085456885C54355233 @default.
• W2085456885 hasConceptScore W2085456885C67705224 @default.
• W2085456885 hasConceptScore W2085456885C76346623 @default.
• W2085456885 hasConceptScore W2085456885C86803240 @default.
• W2085456885 hasIssue "12" @default.
• W2085456885 hasLocation W20854568851 @default.
• W2085456885 hasLocation W20854568852 @default.
• W2085456885 hasOpenAccess W2085456885 @default.
• W2085456885 hasPrimaryLocation W20854568851 @default.
• W2085456885 hasRelatedWork W1527255489 @default.
• W2085456885 hasRelatedWork W2001396871 @default.
• W2085456885 hasRelatedWork W2006162544 @default.
• W2085456885 hasRelatedWork W2047384138 @default.
• W2085456885 hasRelatedWork W2090692139 @default.
• W2085456885 hasRelatedWork W2116808391 @default.
• W2085456885 hasRelatedWork W2353476577 @default.

• next