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- W2085485764 abstract "The Gram-negative pathogen Coxiella burnetii is an intracellular bacterium that replicates within the phagolysosomal vacuoles of eukaryotic cells. This pathogen can infect a wide range of hosts, and is the causative agent of Q fever in humans. Surface-exposed and cell envelope associated proteins are thought to be important for both pathogenesis and protective immunity. Herein, we propose a complementary strategy consisting of (i) in silico prediction and (ii) inventory of the proteomic composition using three enrichment approaches coupled with protein identification. The efficiency of classical Triton X-114 phase partitioning was compared with two novel procedures; isolation of alkaline proteins by liquid-phase IEF, and cell surface enzymatic shaving using biofunctional magnetic beads. Of the 2026 protein sequences analyzed using seven distinct bioinformatic algorithms, 157 were predicted to be outer membrane proteins (OMP) and/or lipoproteins (LP). Using the three enrichment protocols, we identified 196 nonredundant proteins, including 39 predicted OMP and/or LP, 32 unknown or poorly characterized proteins, and 17 effectors of the Type IV secretion system. We additionally identified eight proteins with moonlighting activities, and several proteins apparently peripherally associated with integral or anchored OMP and/or LP." @default.
- W2085485764 created "2016-06-24" @default.
- W2085485764 creator A5012116061 @default.
- W2085485764 creator A5040363021 @default.
- W2085485764 creator A5044884637 @default.
- W2085485764 creator A5084577812 @default.
- W2085485764 creator A5086353622 @default.
- W2085485764 date "2014-07-15" @default.
- W2085485764 modified "2023-10-14" @default.
- W2085485764 title "Identification of<i>Coxiella burnetii</i>surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy" @default.
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