FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085498949> ?p ?o ?g. }

• W2085498949 endingPage "271" @default.
• W2085498949 startingPage "263" @default.
• W2085498949 abstract "Many experiments in plant molecular biology require processing of a large number of RNA samples and in some cases large quantities are required for a single application. In turmeric, a major spice and medicinal plant, a protocol for RNA isolation is not available. The major difficulty encountered while using other popular protocols is the low yield and quality of RNA which hampers the downstream applications like qRT-PCR, cDNA synthesis and micro RNA isolation. Commercial kits though available are costly and were found to be unsuccessful in case of rhizomes and root tissues that are rich in polyphenols, polysaccharides and alkaloids. It was thus felt that a quick, handy and cheap protocol of total RNA isolation from different tissues of turmeric was required for day to day working in our lab. The new protocol utilizes SDS based extraction buffer including β-mercaptoethanol and PVP with sequential acid phenol:chloroform extraction to remove polyphenols and proteins, followed by the purification with sodium acetate to eliminate polysaccharides. The protocol is simple and can be completed in less than 3 h. The RNA yield from rhizome was higher by more than fivefold with both A260/280 and A260/230 ratio in the range of 1.8–2.0. The protocol worked well with leaf, rhizome, pseudostem and root tissues with RIN >7.0 and the isolated RNA could be successfully used for cDNA synthesis, RT-PCR, qRT-PCR and small RNA isolation including microRNA." @default.
• W2085498949 created "2016-06-24" @default.
• W2085498949 creator A5001393138 @default.
• W2085498949 creator A5040879898 @default.
• W2085498949 creator A5045542821 @default.
• W2085498949 creator A5056586077 @default.
• W2085498949 creator A5062622311 @default.
• W2085498949 creator A5063327989 @default.
• W2085498949 creator A5076393201 @default.
• W2085498949 date "2014-01-18" @default.
• W2085498949 modified "2023-10-17" @default.
• W2085498949 title "A simple and efficient protocol for isolation of high quality functional RNA from different tissues of turmeric (Curcuma longa L.)" @default.
• W2085498949 cites W1233505759 @default.
• W2085498949 cites W1564443767 @default.
• W2085498949 cites W1602575883 @default.
• W2085498949 cites W1696199194 @default.
• W2085498949 cites W1967856154 @default.
• W2085498949 cites W1986296268 @default.
• W2085498949 cites W1997209991 @default.
• W2085498949 cites W2010574784 @default.
• W2085498949 cites W2020447874 @default.
• W2085498949 cites W2021528729 @default.
• W2085498949 cites W2029483604 @default.
• W2085498949 cites W2033203682 @default.
• W2085498949 cites W2034643592 @default.
• W2085498949 cites W2038637325 @default.
• W2085498949 cites W2048062229 @default.
• W2085498949 cites W2050732573 @default.
• W2085498949 cites W2054405020 @default.
• W2085498949 cites W2055498144 @default.
• W2085498949 cites W2062745243 @default.
• W2085498949 cites W2064910731 @default.
• W2085498949 cites W2077669412 @default.
• W2085498949 cites W2077972869 @default.
• W2085498949 cites W2081565538 @default.
• W2085498949 cites W2084244231 @default.
• W2085498949 cites W2087215581 @default.
• W2085498949 cites W2092686336 @default.
• W2085498949 cites W2093051731 @default.
• W2085498949 cites W2100652475 @default.
• W2085498949 cites W2109575366 @default.
• W2085498949 cites W2161345634 @default.
• W2085498949 cites W2596619862 @default.
• W2085498949 cites W4254177361 @default.
• W2085498949 cites W4294216491 @default.
• W2085498949 cites W2113175276 @default.
• W2085498949 doi "https://doi.org/10.1007/s12298-013-0218-y" @default.
• W2085498949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3988332" @default.
• W2085498949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24757331" @default.
• W2085498949 hasPublicationYear "2014" @default.
• W2085498949 type Work @default.
• W2085498949 sameAs 2085498949 @default.
• W2085498949 citedByCount "39" @default.
• W2085498949 countsByYear W20854989492015 @default.
• W2085498949 countsByYear W20854989492016 @default.
• W2085498949 countsByYear W20854989492017 @default.
• W2085498949 countsByYear W20854989492018 @default.
• W2085498949 countsByYear W20854989492019 @default.
• W2085498949 countsByYear W20854989492020 @default.
• W2085498949 countsByYear W20854989492021 @default.
• W2085498949 countsByYear W20854989492022 @default.
• W2085498949 countsByYear W20854989492023 @default.
• W2085498949 crossrefType "journal-article" @default.
• W2085498949 hasAuthorship W2085498949A5001393138 @default.
• W2085498949 hasAuthorship W2085498949A5040879898 @default.
• W2085498949 hasAuthorship W2085498949A5045542821 @default.
• W2085498949 hasAuthorship W2085498949A5056586077 @default.
• W2085498949 hasAuthorship W2085498949A5062622311 @default.
• W2085498949 hasAuthorship W2085498949A5063327989 @default.
• W2085498949 hasAuthorship W2085498949A5076393201 @default.
• W2085498949 hasBestOaLocation W20854989492 @default.
• W2085498949 hasConcept C104317684 @default.
• W2085498949 hasConcept C112190416 @default.
• W2085498949 hasConcept C134550120 @default.
• W2085498949 hasConcept C185592680 @default.
• W2085498949 hasConcept C187882448 @default.
• W2085498949 hasConcept C2777230291 @default.
• W2085498949 hasConcept C2778004101 @default.
• W2085498949 hasConcept C43617362 @default.
• W2085498949 hasConcept C4725764 @default.
• W2085498949 hasConcept C55493867 @default.
• W2085498949 hasConcept C556039675 @default.
• W2085498949 hasConcept C59822182 @default.
• W2085498949 hasConcept C67705224 @default.
• W2085498949 hasConcept C70899900 @default.
• W2085498949 hasConcept C71924100 @default.
• W2085498949 hasConcept C86803240 @default.
• W2085498949 hasConceptScore W2085498949C104317684 @default.
• W2085498949 hasConceptScore W2085498949C112190416 @default.
• W2085498949 hasConceptScore W2085498949C134550120 @default.
• W2085498949 hasConceptScore W2085498949C185592680 @default.
• W2085498949 hasConceptScore W2085498949C187882448 @default.
• W2085498949 hasConceptScore W2085498949C2777230291 @default.
• W2085498949 hasConceptScore W2085498949C2778004101 @default.
• W2085498949 hasConceptScore W2085498949C43617362 @default.
• W2085498949 hasConceptScore W2085498949C4725764 @default.
• W2085498949 hasConceptScore W2085498949C55493867 @default.
• W2085498949 hasConceptScore W2085498949C556039675 @default.

• next