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• W2085503524 endingPage "782" @default.
• W2085503524 startingPage "772" @default.
• W2085503524 abstract "White spot syndrome virus (WSSV) is a major threat for farmed shrimp worldwide. RNA interference (RNAi) is the most recent tool against viral diseases. Rab7 silencing effectively inhibited virus infections in juvenile shrimp, but the antiviral effect in brooders remains unknown. This study found a homologue Penaeus monodon Rab7 gene in Litopenaeus vannamei brooders from Mexico. Sequence identity was >99% to a Thai LvRab7 sequence and >94% to Rab7 sequences from P. monodon or Marsupenaeus japonicus. Animals treated with a partial (494 bp) or a complete (618 bp) LvRab7 dsRNA sequences and challenged 48 h post treatment (hpt) with a high WSSV dose showed 80–88% mortality respectively. Shrimp treated with 4 or 20 μg LvRab7 dsRNA and challenged with a WSSV high dose had 80% mortality each, but it was reduced to 33% and 40%, respectively, with a low dose. Efficacy of dsRNA to reduce shrimp mortality was dependent on virus dose used regardless of dsRNA concentration. A significant reduction in LvRab7 mRNA levels was observed at 120 hpt. In conclusion, silencing LvRab7 in brooders showed a mild antiviral effect against a WSSV challenge at 48 hpt." @default.
• W2085503524 created "2016-06-24" @default.
• W2085503524 creator A5031317272 @default.
• W2085503524 creator A5043027458 @default.
• W2085503524 creator A5053495063 @default.
• W2085503524 creator A5070731418 @default.
• W2085503524 date "2012-02-09" @default.
• W2085503524 modified "2023-10-18" @default.
• W2085503524 title "Silencing Pacific white shrimp<i>Litopenaeus vannamei</i>LvRab7 reduces mortality in brooders challenged with white spot syndrome virus" @default.
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• W2085503524 doi "https://doi.org/10.1111/j.1365-2109.2011.03084.x" @default.
• W2085503524 hasPublicationYear "2012" @default.
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