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• W2085511602 abstract "A cDNA encoding human urokinase-type plasminogen activator was inserted downstream from the polyhedrin promoter of the baculovirus Autographa californica nuclear polyhedrosis virus. A protein of similar Mr to urokinase (UK) was synthesized and approx. 90% was secreted from recombinant virus-infected Spodoptera frugiperda cells. Zymography and Western blotting analysis of the insect-derived protein demonstrated that it was comprised solely of the high-Mr form of UK. No low-Mr UK was detected. Amidolytic activity assays showed that 96% of the insect cell-derived UK was in the single-chain proenzyme form. The yield of UK from insect cells was 1986 international units/ml/10(6) infected cells." @default.
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• W2085511602 date "1991-10-01" @default.
• W2085511602 modified "2023-10-16" @default.
• W2085511602 title "Secretion of single-chain urokinase-type plasminogen activator from insect cells" @default.
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• W2085511602 doi "https://doi.org/10.1016/0378-1119(91)90194-g" @default.
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