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• W2085562016 abstract "Total internal reflection fluorescence microscopy (TIRFM) was investigated for enhanced detection sensitivity of cardiac troponin I (cTnI) on a gold nano-patterned protein chip. A 4×5 nanoarray patterning of a gold chip based on a single-molecule sandwich immunoassay was prepared with a spot diameter of 500 nm on glass substrates formed via electron-beam nanolithography. Quantitative trace analysis was possible as a function of increasing total internal reflection fluorescence intensity in the range of 84 aM-350 pM at prism-type and 35 aM-350 pM at objective-type TIRFM. Although using same gold nano-patterned cTnI chips, however, the objective-type TIRFM showed enhanced detection sensitivities 2.4 times better than the prism-type due to weaker gold quenching. This result is also about 7000 times lower in the detection limit compared with those obtained by conventional commercial sandwich enzyme-linked immunosorbent assay techniques." @default.
• W2085562016 created "2016-06-24" @default.
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• W2085562016 date "2013-01-01" @default.
• W2085562016 modified "2023-10-14" @default.
• W2085562016 title "Quenching effect on gold nano-patterned cardiac troponin I chip by total internal reflection fluorescence microscopy" @default.
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• W2085562016 doi "https://doi.org/10.1016/j.talanta.2012.11.014" @default.
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