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• W2085572800 abstract "Abstract Secondary monolayer cultures from whole mouse embryos were infected at 39° with ts -P155, an early temperature-sensitive mutant of polyoma virus that transforms but replicates poorly at this temperature ( W. Eckhart, 1969 , Virology 38 , 120–125; W. Eckhart, 1974 , Cold Spring Harbor Symp. Quant. Biol. 39 , 37–40). The cultures were then passaged at 39° in the presence of polyoma virus antiserum until sufficient cell degeneration occurred to allow detection of foci of cells exhibiting lack of contact-inhibition. From one such focus, a cell strain—designated Cyp—was derived and cloned at 39°. Whether cloned or uncloned, Cyp cells all seem to exhibit essentially the same phenotype. At 39°, they display several properties of transformed cells and, as indicated by immunofluorescence, synthesize polyoma T antigen. They do not produce any readily detectable amounts of viral DNA or V antigen and only low amounts of viral hemagglutinin at 39°, although they are still fully permissive for polyoma virus replication at this temperature. Upon transfer to 33°, Cyp cells undergo a massive cytopathic effect detectable within 30 to 34 hr and produce large amounts of infectious, temperature-sensitive polyoma virus. Viral DNA accumulating in Cyp cells after temperature shift-down consists primarily of covalently closed monomers and, upon cleavage by Hpa II, yields fragments characteristic of the polyoma virus strain from which the ts -P155 mutant had been derived. When present in the medium during temperature shift-down, polyoma virus antiserum neither curtails the cytopathic effect nor depresses viral DNA synthesis, although it suppresses virus production. These observations are in agreement with the results of infectious center assays, suggesting that most Cyp cells produce virus following transfer to 33°." @default.
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• W2085572800 date "1978-07-01" @default.
• W2085572800 modified "2023-09-25" @default.
• W2085572800 title "A mouse embryo cell line carrying an inducible, temperature-sensitive, polyoma virus genome" @default.
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• W2085572800 doi "https://doi.org/10.1016/0042-6822(78)90291-x" @default.
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