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• W2085576183 abstract "Two different strategies for coupling liquid chromatography with atmospheric pressure matrix assisted laser desorption/ionization (AP MALDI) are presented. The first method is flow-injection liquid AP UV-MALDI. Compared with previous similar research, the detection limit was improved 10 times to 8.3 fmol using a solution of 50 nM peptide with 25 mM α-cyano-4-hydroxycinnamic acid. The applicability of this method to measure oligosaccharides, actinomycin antibiotics, antibiotics, phosphopeptides, and proteins is demonstrated. The upper mass limit achieved with the current instrumentation is 6,500 Da (doubly charged cytochrome c). The feasibility of a second strategy based on single-droplet IR AP MALDI is demonstrated here. Aqueous peptide solutions were successfully measured by this method." @default.
• W2085576183 created "2016-06-24" @default.
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• W2085576183 date "2005-10-28" @default.
• W2085576183 modified "2023-10-02" @default.
• W2085576183 title "Interfacing liquid chromatography with atmospheric pressure MALDI-MS" @default.
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• W2085576183 doi "https://doi.org/10.1007/s00216-005-0098-2" @default.
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