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• W2085655662 startingPage "1331" @default.
• W2085655662 abstract "Cells of Escherichia coli containing the plasmid F were gamma-irradiated with various doses to introduce determined numbers of single-strand breaks in the F DNA. The cells were then incubated to permit repair of the breaks while DNA gyrase was inhibited with coumermycin to limit restoration of any relaxed supercoil. Repaired, covalently continuous F DNA was isolated and its superhelical density was measured by two different methods. Both indicated that a major part (50-60%) of the negative superhelical turns were maintained in the repaired molecules, suggesting that the supercoils are restrained in vivo." @default.
• W2085655662 created "2016-06-24" @default.
• W2085655662 creator A5062600178 @default.
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• W2085655662 date "1980-03-01" @default.
• W2085655662 modified "2023-09-23" @default.
• W2085655662 title "Supercoils in prokaryotic DNA restrained in vivo." @default.
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• W2085655662 doi "https://doi.org/10.1073/pnas.77.3.1331" @default.
• W2085655662 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/348488" @default.
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