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• W2085665619 endingPage "479" @default.
• W2085665619 startingPage "469" @default.
• W2085665619 abstract "During cell division, Nuclear Pore Complexes (NPCs) are broken down into protein subcomplexes that are the basis for reassembly in daughter cells. This is the driving force for the establishment of an in vitro reconstitution system to study aspects of NPC reassembly. In this study, nuclear envelope (NE) was isolated from HeLa cells. NE was treated with increasing concentrations of heparin to extract nucleoporins (Nups) for the production of ghost pores which are pores severely deficient in Nups, while still containing Pore Membrane proteins (POM) needed to anchor the NPC. Ghost pores have been subjected to incubation with previously stripped Nups and some re-binding has been shown to occur by western blot analysis. This in vitro assay provides a powerful tool to investigate the protein-protein interactions of NPC reassembly from a human cell line. Through a better understanding of the process of NPC reassembly, we can continue to piece together the puzzle of this macromolecular structure. It is most advantageous to establish a straightforward reconstitution procedure at the mammalian level." @default.
• W2085665619 created "2016-06-24" @default.
• W2085665619 creator A5054923007 @default.
• W2085665619 creator A5078877449 @default.
• W2085665619 date "2012-10-09" @default.
• W2085665619 modified "2023-09-27" @default.
• W2085665619 title "Depletion of nucleoporins from HeLa nuclear pore complexes to facilitate the production of ghost pores for in vitro reconstitution" @default.
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• W2085665619 doi "https://doi.org/10.1007/s10616-012-9501-y" @default.
• W2085665619 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3720962" @default.
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• W2085665619 hasPublicationYear "2012" @default.
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