FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085673406> ?p ?o ?g. }

• W2085673406 endingPage "133" @default.
• W2085673406 startingPage "113" @default.
• W2085673406 abstract "The binding properties of rigid heparin sorbents, synthesised by end-point-attachment of heparin onto aminopropyl-derivatised silica through reductive amination, were characterised through batch-adsorption studies employing human plasma fractions containing thrombin. Thrombin was quantified using a chromogenic assay that had been specially modified for these studies. These investigations yielded information regarding the maximum adsorption capacities/stoichiometries and binding affinities for thrombin present in complex protein mixtures. Of the two types of heparin-silica evaluated, heparin-Fractosil 1000, with a pore size of 1000 Å, displayed a capacity of 2.4 mol of thrombin/mol of heparin (mol T-mol H). This stoichiometry was significantly higher than the value of 1.8 mol T-mol H obtained for the commercial soft gel heparin-Sepharose CL-6B. Furthermore, the heparin-Fractosil 1000 sorbents were superior in capacity and binding site accessibility to heparin-LiChroprep Si60 sorbents, where the smaller pore size of 60 Å largely restricts the ligand-protein interactions to the outer surface of the sorbent particles. Nevertheless, heparin-LiChroprep Si60 sorbents were useful, in that they simulated a non-porous particle system, in which intra-pore diffusion effects are eliminated. The batch adsorption results with these sorbents indicated that the adsorption involved both high and low binding affinity characteristics. This bimodal binding mechanism was also evident with the commercial heparin-Sepharose sorbent. Binding stoichiometries and affinities in the high concentration range were similar to values reported for a largely non-specific electrostatic thrombin-heparin interaction. Dissociation constants in the nanomolar range were observed in the low concentrations range. This stronger binding affinity is more similar to highly specific bio-affinity interactions. Thus, the results indicated that heparin-thrombin interactions with these systems involve both a weak electrostatic and a strong biospecific interaction component." @default.
• W2085673406 created "2016-06-24" @default.
• W2085673406 creator A5003173236 @default.
• W2085673406 creator A5077463097 @default.
• W2085673406 date "1997-02-01" @default.
• W2085673406 modified "2023-09-27" @default.
• W2085673406 title "Characterisation of silica-based heparin affinity sorbents from equilibrium binding studies on plasma fractions containing thrombin" @default.
• W2085673406 cites W1482439495 @default.
• W2085673406 cites W1499963171 @default.
• W2085673406 cites W1524266868 @default.
• W2085673406 cites W1561931582 @default.
• W2085673406 cites W1567264882 @default.
• W2085673406 cites W1569621897 @default.
• W2085673406 cites W1608119064 @default.
• W2085673406 cites W1618720360 @default.
• W2085673406 cites W1720895391 @default.
• W2085673406 cites W194739440 @default.
• W2085673406 cites W1963625808 @default.
• W2085673406 cites W1972717861 @default.
• W2085673406 cites W1981551601 @default.
• W2085673406 cites W1984547849 @default.
• W2085673406 cites W1988505276 @default.
• W2085673406 cites W1993736955 @default.
• W2085673406 cites W1998891524 @default.
• W2085673406 cites W2011237929 @default.
• W2085673406 cites W2022364167 @default.
• W2085673406 cites W2028372667 @default.
• W2085673406 cites W2046197386 @default.
• W2085673406 cites W2047163160 @default.
• W2085673406 cites W2048788857 @default.
• W2085673406 cites W2050265290 @default.
• W2085673406 cites W2057657597 @default.
• W2085673406 cites W2058577599 @default.
• W2085673406 cites W2064239932 @default.
• W2085673406 cites W2067801069 @default.
• W2085673406 cites W2071626236 @default.
• W2085673406 cites W2084354947 @default.
• W2085673406 cites W2139481768 @default.
• W2085673406 cites W2145367315 @default.
• W2085673406 cites W2334283888 @default.
• W2085673406 cites W2406887327 @default.
• W2085673406 cites W4255682245 @default.
• W2085673406 doi "https://doi.org/10.1016/s0021-9673(96)00957-0" @default.
• W2085673406 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9098971" @default.
• W2085673406 hasPublicationYear "1997" @default.
• W2085673406 type Work @default.
• W2085673406 sameAs 2085673406 @default.
• W2085673406 citedByCount "14" @default.
• W2085673406 crossrefType "journal-article" @default.
• W2085673406 hasAuthorship W2085673406A5003173236 @default.
• W2085673406 hasAuthorship W2085673406A5077463097 @default.
• W2085673406 hasConcept C144082473 @default.
• W2085673406 hasConcept C150394285 @default.
• W2085673406 hasConcept C170493617 @default.
• W2085673406 hasConcept C178790620 @default.
• W2085673406 hasConcept C179247698 @default.
• W2085673406 hasConcept C181199279 @default.
• W2085673406 hasConcept C185592680 @default.
• W2085673406 hasConcept C203014093 @default.
• W2085673406 hasConcept C2777292125 @default.
• W2085673406 hasConcept C2777557582 @default.
• W2085673406 hasConcept C2777899863 @default.
• W2085673406 hasConcept C43617362 @default.
• W2085673406 hasConcept C55493867 @default.
• W2085673406 hasConcept C74998103 @default.
• W2085673406 hasConcept C86803240 @default.
• W2085673406 hasConcept C89560881 @default.
• W2085673406 hasConceptScore W2085673406C144082473 @default.
• W2085673406 hasConceptScore W2085673406C150394285 @default.
• W2085673406 hasConceptScore W2085673406C170493617 @default.
• W2085673406 hasConceptScore W2085673406C178790620 @default.
• W2085673406 hasConceptScore W2085673406C179247698 @default.
• W2085673406 hasConceptScore W2085673406C181199279 @default.
• W2085673406 hasConceptScore W2085673406C185592680 @default.
• W2085673406 hasConceptScore W2085673406C203014093 @default.
• W2085673406 hasConceptScore W2085673406C2777292125 @default.
• W2085673406 hasConceptScore W2085673406C2777557582 @default.
• W2085673406 hasConceptScore W2085673406C2777899863 @default.
• W2085673406 hasConceptScore W2085673406C43617362 @default.
• W2085673406 hasConceptScore W2085673406C55493867 @default.
• W2085673406 hasConceptScore W2085673406C74998103 @default.
• W2085673406 hasConceptScore W2085673406C86803240 @default.
• W2085673406 hasConceptScore W2085673406C89560881 @default.
• W2085673406 hasIssue "1-2" @default.
• W2085673406 hasLocation W20856734061 @default.
• W2085673406 hasLocation W20856734062 @default.
• W2085673406 hasOpenAccess W2085673406 @default.
• W2085673406 hasPrimaryLocation W20856734061 @default.
• W2085673406 hasRelatedWork W1968931370 @default.
• W2085673406 hasRelatedWork W1983861917 @default.
• W2085673406 hasRelatedWork W2007463197 @default.
• W2085673406 hasRelatedWork W2028372667 @default.
• W2085673406 hasRelatedWork W2034049967 @default.
• W2085673406 hasRelatedWork W2040920814 @default.
• W2085673406 hasRelatedWork W2043404328 @default.
• W2085673406 hasRelatedWork W2071626236 @default.
• W2085673406 hasRelatedWork W2168925541 @default.
• W2085673406 hasRelatedWork W2463103678 @default.

• next