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- W2085684689 abstract "Macromolecular crowding in living cells influences diffusion, intermolecular interactions, protein folding and intracellular transport. We investigated the rotational and translational diffusion of chymotrypsin inhibitor 2 (CI2) and enhanced green fluorescent protein (EGFP) in biomimetic crowded environments that are created from synthetic polymers (Ficoll-70, Ficoll-400) or proteins (bovine serum albumin and ovalbumin) and compared these with diffusion in homogeneous solvents (buffer and glycerol). Time-resolved fluorescence anisotropy (ps-ns) and fluorescence correlation spectroscopy (us-s) were used to elucidate the effects of nonspecific binding on the diffusion mechanism of size-dependent tracers. The measured rotational-to-translational diffusion coefficient ratios for CI2 and EGFP indicate that the diffusion deviates from the Stokes-Einstein model (i.e., non-Brownian), depending on the type of crowding agent and the tracer. We attribute these findings to nonspecific interactions between the tracer and crowding agents, as well as microviscosity heterogeneities in the crowded environments. Our real-time, multiscale diffusion measurements for CI2 are compared with recent NMR experiment under similar experimental conditions." @default.
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- W2085684689 date "2014-01-01" @default.
- W2085684689 modified "2023-09-28" @default.
- W2085684689 title "Macromolecular Crowding Effects on the Multiscale Diffusion of Single Molecules" @default.
- W2085684689 doi "https://doi.org/10.1016/j.bpj.2013.11.4057" @default.
- W2085684689 hasPublicationYear "2014" @default.
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