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• W2085694005 abstract "A cell culture system that supports the differentiation of male germ cells through meiosis is described. It takes advantage of the properties of a cell line, 15P-1, established from testicular cells of transgenic mice that express the large T protein of polyoma virus in the seminiferous epithelium. This line exhibits features characteristics of Sertoli cells, including transcription of the Wilms' tumor (WT1) and Steel genes. Cells of the 15P-1 type support the meiotic and postmeiotic differentiation in cocultures of diploid premeiotic germ cells into haploid spermatids expressing the protamine (Prm-1) gene. When cocultured with 15P-1 cells, testicular cells explanted from immature 9-day-old animals, before the onset of the first meiosis, generated tetrads of haploid cells with the morphology of round spermatids and initiated protamine transcription." @default.
• W2085694005 created "2016-06-24" @default.
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• W2085694005 date "1993-12-01" @default.
• W2085694005 modified "2023-10-14" @default.
• W2085694005 title "Transmeiotic differentiation of male germ cells in culture" @default.
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• W2085694005 doi "https://doi.org/10.1016/0092-8674(93)90543-y" @default.
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