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- W2085702862 abstract "SUMMARY: Two different bands with laccase activity were obtained after nondenaturing PAGE of the culture filtrate of Pleurotus ostreatus. Immunoblot analysis revealed that antisera raised against laccase I were not reactive to laccase II. Laccase I, which exhibited faster mobility on nondenaturing polyacrylamide gel, was purified 42·9-fold with an overall yield of 10·8%. Gel filtration and SDS-PAGE revealed that laccase I is a single polypeptide with a molecular mass of approximately 64 kDa. Laccase I contained 12·5% carbohydrate by weight and 3·9 mol copper (mol protein)−1. The absorption spectrum of laccase I showed a type 1 signal at 605 nm and EPR spectra showed that the parameters of the type 1 and type 2 Cu signals were g ‖ = 2·197 and A ‖ = 0·009 cm−1, and g ‖ = 2·263 and A ‖ = 0·0176 cm−1, respectively. The data obtained from the pH profiles suggested that two ionization groups, whose pK a values were 5·60–5·70 and 6·70–6·85, may play an important role in the active site of laccase I as the ligand of copper metal. The optimal pH and temperature for the activity of laccase I were 6·0–6·5 and 30–35 °C, respectively. The enzyme had affinity for various lignin-related phenolic compounds: the K m values for ferulic acid and syringic acid were 48 and 89 μM, respectively. EPR spectroscopic study of the action of laccase I on 3,5-dimethoxy-5-hydroxyacetophenone indicated that this enzyme catalyses single electron transfer with the formation of the phenoxy radical as an intermediate." @default.
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- W2085702862 date "1995-02-01" @default.
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- W2085702862 title "Single electron transfer by an extracellular laccase from the white-rot fungus Pleurotus ostreatus" @default.
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- W2085702862 doi "https://doi.org/10.1099/13500872-141-2-393" @default.
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