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• W2085705809 startingPage "1823" @default.
• W2085705809 abstract "Host cell invasion in the Apicomplexa is unique in its dependency on a parasite actin-driven machinery and in the exclusion of most host cell membrane proteins during parasitophorous vacuole (PV) formation. This exclusion occurs at a junction between host cell and parasite plasma membranes that has been called the moving junction, a circumferential zone which forms at the apical tip of the parasite, moves backward and eventually pinches the PV from the host cell membrane. Despite having been described by electron microscopic studies 30 years ago, the molecular nature of this singular structure is still enigmatic. We have obtained a monoclonal antibody that recognizes the moving junction of invading tachyzoites of Toxoplasma gondii, in a pattern clearly distinct from those described so far for microneme and rhoptry proteins. The protein recognized by this antibody has been affinity purified. Mass spectrometry analysis showed that it is a rhoptry neck protein (RON4), a hypothetical protein with homologues restricted to Apicomplexa. Our findings reveals for the first time the participation of rhoptry neck proteins in moving junction formation and strongly suggest the conservation of this structure at the molecular level among Apicomplexa." @default.
• W2085705809 created "2016-06-24" @default.
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• W2085705809 date "2005-12-01" @default.
• W2085705809 modified "2023-09-25" @default.
• W2085705809 title "The rhoptry neck protein RON4 relocalizes at the moving junction during Toxoplasma gondii invasion" @default.
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• W2085705809 doi "https://doi.org/10.1111/j.1462-5822.2005.00646.x" @default.
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