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• W2085708567 abstract "Arabinose, fructose, galactose, myo-inositol, lyxose, mannose, ribose, and xylose were incubated individually and with glucose in the presence of Aspergillus niger glucoamylase at pH 4.5 and 45 degrees C. Glucoamylase condenses galactose, glucose, and mannose individually into disaccharides. It also produces mixed disaccharides when each of the eight carbohydrates is incubated with glucose. Many products were identified by gas chromatography of the derivatized reaction mixtures followed by mass spectroscopy of the individual chromatographic peaks. Galacto-, gluco-, or mannopyranosyl rings appear to be present at the nonreducing ends of all the disaccharides produced. Molecules linked through primary hydroxyl groups have the highest equilibrium constants of all products formed, since these bonds are thermodynamically favored. However, glucoamylase is capable of forming bonds with many available hydroxyl groups, as previously demonstrated when it was incubated with glucose alone. Formation rates of different bonds linking different residues vary widely. These results demonstrate that glucoamylase has a wide selectivity toward residues it will condense into disaccharides and toward bonds it will form between them." @default.
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• W2085708567 date "1997-10-05" @default.
• W2085708567 modified "2023-09-27" @default.
• W2085708567 title "Kinetics and equilibria of condensation reactions between monosaccharide pairs catalyzed byAspergillus niger glucoamylase" @default.
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• W2085708567 doi "https://doi.org/10.1002/(sici)1097-0290(19971005)56:1<9::aid-bit2>3.0.co;2-o" @default.
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