FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2085718441> ?p ?o ?g. }

• W2085718441 endingPage "238" @default.
• W2085718441 startingPage "231" @default.
• W2085718441 abstract "Molecular methods are used increasingly for the detection of Toxoplasma gondii infection. This study developed a rapid, sensitive, and specific conventional triplex PCR for the detection of the B1 gene and ITS1 region of T. gondii using newly designed primers and an internal control based on the Vibrio cholerae HemM gene. The annealing temperature and concentrations of the primers, MgCl2, and dNTPs were optimized. Two sets of primers (set 1 and 2) were tested, which contained different segments of the T. gondii B1 gene, 529 repeat region and ITS1 region. A series of sensitivity tests were performed using parasite DNA, whole parasites, and spiked human body fluids. Specificity tests were performed using DNA from common protozoa and bacteria. The newly developed assay based on set 2 primers was found to be specific and sensitive. The test was capable of detecting as little as 10 pg T. gondii DNA, 104 tachyzoites in spiked body fluids, and T. gondii DNA in the organ tissues of experimentally infected mice. The assay developed in this study will be useful for the laboratory detection of T. gondii infection." @default.
• W2085718441 created "2016-06-24" @default.
• W2085718441 creator A5019021563 @default.
• W2085718441 creator A5021901138 @default.
• W2085718441 creator A5041624674 @default.
• W2085718441 date "2012-06-01" @default.
• W2085718441 modified "2023-09-23" @default.
• W2085718441 title "Triplex PCR using new primers for the detection of Toxoplasma gondii" @default.
• W2085718441 cites W1592769832 @default.
• W2085718441 cites W1876241751 @default.
• W2085718441 cites W1967340495 @default.
• W2085718441 cites W1999379064 @default.
• W2085718441 cites W2009003303 @default.
• W2085718441 cites W2010496195 @default.
• W2085718441 cites W2010717700 @default.
• W2085718441 cites W2022459353 @default.
• W2085718441 cites W2038283040 @default.
• W2085718441 cites W2043197861 @default.
• W2085718441 cites W2049855073 @default.
• W2085718441 cites W2066104965 @default.
• W2085718441 cites W2071077793 @default.
• W2085718441 cites W2094282307 @default.
• W2085718441 cites W2112832037 @default.
• W2085718441 cites W2117492620 @default.
• W2085718441 cites W2131122023 @default.
• W2085718441 cites W2136232438 @default.
• W2085718441 cites W2150243696 @default.
• W2085718441 cites W4234856234 @default.
• W2085718441 doi "https://doi.org/10.1016/j.exppara.2012.04.009" @default.
• W2085718441 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22561042" @default.
• W2085718441 hasPublicationYear "2012" @default.
• W2085718441 type Work @default.
• W2085718441 sameAs 2085718441 @default.
• W2085718441 citedByCount "28" @default.
• W2085718441 countsByYear W20857184412013 @default.
• W2085718441 countsByYear W20857184412014 @default.
• W2085718441 countsByYear W20857184412015 @default.
• W2085718441 countsByYear W20857184412016 @default.
• W2085718441 countsByYear W20857184412017 @default.
• W2085718441 countsByYear W20857184412018 @default.
• W2085718441 countsByYear W20857184412019 @default.
• W2085718441 countsByYear W20857184412020 @default.
• W2085718441 countsByYear W20857184412021 @default.
• W2085718441 countsByYear W20857184412022 @default.
• W2085718441 countsByYear W20857184412023 @default.
• W2085718441 crossrefType "journal-article" @default.
• W2085718441 hasAuthorship W2085718441A5019021563 @default.
• W2085718441 hasAuthorship W2085718441A5021901138 @default.
• W2085718441 hasAuthorship W2085718441A5041624674 @default.
• W2085718441 hasBestOaLocation W20857184411 @default.
• W2085718441 hasConcept C104317684 @default.
• W2085718441 hasConcept C136764020 @default.
• W2085718441 hasConcept C153911025 @default.
• W2085718441 hasConcept C159047783 @default.
• W2085718441 hasConcept C159654299 @default.
• W2085718441 hasConcept C203014093 @default.
• W2085718441 hasConcept C2778048844 @default.
• W2085718441 hasConcept C2778062946 @default.
• W2085718441 hasConcept C2778128430 @default.
• W2085718441 hasConcept C2778371730 @default.
• W2085718441 hasConcept C2778566189 @default.
• W2085718441 hasConcept C2781023229 @default.
• W2085718441 hasConcept C41008148 @default.
• W2085718441 hasConcept C49105822 @default.
• W2085718441 hasConcept C54355233 @default.
• W2085718441 hasConcept C552990157 @default.
• W2085718441 hasConcept C71928629 @default.
• W2085718441 hasConcept C86803240 @default.
• W2085718441 hasConcept C89423630 @default.
• W2085718441 hasConceptScore W2085718441C104317684 @default.
• W2085718441 hasConceptScore W2085718441C136764020 @default.
• W2085718441 hasConceptScore W2085718441C153911025 @default.
• W2085718441 hasConceptScore W2085718441C159047783 @default.
• W2085718441 hasConceptScore W2085718441C159654299 @default.
• W2085718441 hasConceptScore W2085718441C203014093 @default.
• W2085718441 hasConceptScore W2085718441C2778048844 @default.
• W2085718441 hasConceptScore W2085718441C2778062946 @default.
• W2085718441 hasConceptScore W2085718441C2778128430 @default.
• W2085718441 hasConceptScore W2085718441C2778371730 @default.
• W2085718441 hasConceptScore W2085718441C2778566189 @default.
• W2085718441 hasConceptScore W2085718441C2781023229 @default.
• W2085718441 hasConceptScore W2085718441C41008148 @default.
• W2085718441 hasConceptScore W2085718441C49105822 @default.
• W2085718441 hasConceptScore W2085718441C54355233 @default.
• W2085718441 hasConceptScore W2085718441C552990157 @default.
• W2085718441 hasConceptScore W2085718441C71928629 @default.
• W2085718441 hasConceptScore W2085718441C86803240 @default.
• W2085718441 hasConceptScore W2085718441C89423630 @default.
• W2085718441 hasIssue "2" @default.
• W2085718441 hasLocation W20857184411 @default.
• W2085718441 hasLocation W20857184412 @default.
• W2085718441 hasOpenAccess W2085718441 @default.
• W2085718441 hasPrimaryLocation W20857184411 @default.
• W2085718441 hasRelatedWork W1876241751 @default.
• W2085718441 hasRelatedWork W1966332012 @default.
• W2085718441 hasRelatedWork W1977729962 @default.
• W2085718441 hasRelatedWork W2093705323 @default.
• W2085718441 hasRelatedWork W2124415434 @default.

• next